Core D: Chemistry and Analytical Core (CAC)

摘要：化学和分析核心 化学和分析核心（CAC）是UNC-SRP的关键研究支持核心，提供 具有测量有毒物质的专业知识和分析能力，优先考虑无机砷（iAs）， 共存污染物，并表征其对代谢功能障碍/糖尿病的影响。CAC提供 分析专业知识和服务，获得最先进的金属测量仪器， 微生物组和生物标志物/代谢组学分析，以及尖端研究方法的培训。的 CAC为项目1-5提供了生成关键数据集所需的工具和专业知识， 确定减少iAs暴露和预防iAs诱导代谢功能障碍的新策略。食典委 工作人员通过使用多功能Agilent电感耦合等离子体质谱仪完成任务 采用电感耦合等离子体质谱（ICP-MS）和氢化物发生-低温捕集-原子吸收光谱仪（HG-CT-AAS）对全元素进行分析 和形态分析，高灵敏度的AB Sciex和Thermo TSQ三重四极杆MS用于生物标志物 定量和高分辨率Thermo QE HF Orbitrap MS，用于代谢组学分析和代谢产物分析 识别.此外，CAC还建立了通过16 S rRNA基因进行微生物组分析的专业知识 测序和鸟枪宏基因组学。化学和分析核心的总体目标是 为UNC-SRP项目提供分析基础设施、仪器、分析服务， 相关培训。将追求三个具体目标，包括：目标1。制定和实施分析 方法学，侧重于金属及其代谢物/物质的分离、检测和定量; 目标二。通过宏基因组学检查毒物诱导的肠道微生物组干扰和代谢破坏 和代谢组学;以及目标3。为生物医学和环境科学项目提供咨询， 为研究生和博士后提供先进分析设备支持性环境。 食典委支持《联合国预防犯罪和刑事司法改革方案》的总体主题：“确定减少接触国际麻醉品的新方法， 阐明iAs诱导的代谢功能障碍的潜在机制，以预防疾病。!的 CAC活动通过金属分析方面的专业知识促进所有项目和岩心之间的互动， 微生物组和生物标志物/代谢组学分析，这对指导项目的研究策略至关重要。 每一个组件都到位，包括最先进的设备，严格的QA/QC计划，经验丰富的 在人员和强有力的领导团队和机构支持下，CAC将在建立联系方面发挥关键作用 中心的研究人员。 !

ABSTRACT: CHEMISTRY AND ANALYICAL CORE The Chemistry and Analytical Core (CAC) is a critical research support core for the UNC-SRP, providing expertise and analytical capabilities to measure toxic substances with prioritization of inorganic arsenic (iAs) and co-occurring contaminants and characterize their effects on metabolic dysfunction/diabetes. The CAC offers analytical expertise and services, access to state-of-the-art instrumentation for metals measurements, microbiome and biomarker/metabolomics analysis, and training in cutting-edge research methodologies. The CAC provides Projects 1-5 with the tools and expertise needed to generate critical data sets that will help to identify novel strategies to reduce iAs exposure and to prevent iAs-induced metabolic dysfunction. The CAC staff accomplishes tasks through utilization of a versatile Agilent inductively coupled plasma mass spectrometry (ICP-MS) and a hydride generation-cryotrapping-atomic absorption spectrometer (HG-CT-AAS) for total element and speciation analysis, highly sensitive AB Sciex and Thermo TSQ triple quadrupole MS for biomarker quantitation, and high resolution Thermo QE HF Orbitrap MS for metabolomics profiling and metabolite identification. In addition, the CAC has established expertise in microbiome analysis via 16S rRNA gene sequencing and shotgun metagenomics. The overall objective of the Chemistry and Analytical Core is to provide the UNC-SRP Projects with analytical infrastructure, instrumentation, analytical service and related training. Three specific aims will be pursued, including: Aim 1. Develop and implement analytical methodologies, with a focus on separation, detection and quantitation of metals and their metabolites/species; Aim 2. Examine toxicant-induced gut microbiome perturbation and metabolic disruption through metagenomics and metabolomics; and Aim 3. Provide consultation to Biomedical and Environmental Science Projects and a supportive environment for graduate student and postdoctoral training on state-of-the-art analytical equipment. The CAC supports the overall theme of the UNC-SRP: “Identifying novel methods to reduce iAs exposure and elucidating mechanisms underlying iAs-induced metabolic dysfunction with a vision for disease prevention.” !The CAC activities promote interaction among all Projects and Cores through expertise in metals analyses, microbiome and biomarker/metabolomics analysis that is critical in guiding the Projects’ research strategies. With every component in place, including state-of-the-art equipment, rigorous QA/QC plans, highly experienced personnel and a strong leadership team and institutional support, the CAC will play a key role in forging links among the Center researchers. !