Translational Biomarker Analytical Core

翻译生物标志物分析核心（TBAC）实验室向DRC提供 研究人员分析技术，以支持糖尿病，胰岛素抵抗， 人和动物中糖尿病中间代谢和医学后果 模型提供的服务最好在核心实验室进行，因为它们需要研究人员 难以或不切实际建立的咨询、特殊仪器和方法， 个别实验室。此外，这种安排允许协调注意可靠性， 所用测定的重现性。在葡萄糖稳态、胰岛素分泌、 胰岛素抵抗、血脂异常、激素作用、分子遗传学或药物作用， 能够监测激素，如胰岛素、C肽、胰高血糖素、GLP-1、GIP、PYY、皮质醇和 以及诸如葡萄糖、FFA、甘油、酮、氨基酸、脂质的代谢物 更详细的代谢指纹评估。随着越来越多的技术 开发和验证新型疾病生物标志物的能力，核心的关键功能之一是 尽快开发和开创这些新方法，使研究基地能够利用 这些化验。TBAC的具体目标是：1）满足对动态的高需求 研究基地提供一系列生物测定，提高效率、质量和再现性， 最小化成本。2)通过支持新检测方法的开发来满足成员的需求 以及利用核心工作人员专业知识的方法。3)为便利获得有针对性的和 由哥伦比亚欧文生物标志物实验室进行的非靶向代谢组学/脂质组学测定， 新扩展的代谢组学核心4)为了方便接触生物信息学专家， 适当解释代谢组学/脂质组学所需的研究和途径分析 测定和稳定同位素动力学研究。5)向研究人员提供专家建议， 他们的同事在分析方法，实验设计和数据解释的最先进的 糖尿病的表型6)培训初级研究员和博士后研究员的实践技能， 核心方法，并酌情将分析方法转移到自己的实验室。 研究基地之间的合作，宣传可用资源，并开发和/或促进 研究人员获得新技术，如基于MS的分析和量化方法 代谢物。TBAC的分析和咨询能力与其他DRC密切相关 提供定量代谢分析的综合方法。

The Translational Biomarker Analytical Core (TBAC) Laboratory makes available to DRC investigators analytical techniques to support studies of diabetes mellitus, insulin resistance, intermediary metabolism, and medical consequences of diabetes in human subjects and animal models. The services provided are best based in a Core Laboratory because they require investigator consultation, special instrumentation and methods that are difficult or impracticable to establish in individual laboratories. In addition, this arrangement allows for coordinated attention to reliability and reproducibility of the assays employed. In investigations of glucose homeostasis, insulin secretion, insulin resistance, dyslipidemia, hormone action, molecular genetics, or drug effects, it is essential to be able to monitor hormones such as insulin, C-peptide, glucagon, GLP-1, GIP, PYY, cortisol and catecholamines and such metabolites as glucose, FFA, glycerol, ketones, amino acids, lipids, as well as more detailed assessment of the metabolomics fingerprint. With the increasing technical capabilities to develop and validate novel disease biomarkers, one of the key functions of the Core is to expeditiously develop and inaugurate these new methods so that the research base can avail itself of these assays. The SPECIFIC AIMS of the TBAC are: 1) To meet the high demand for a dynamic range of bioassays by the research base, enhancing efficiency, quality, and reproducibility, while minimizing costs. 2) To respond to members’ needs by supporting the development of new assays and methods that leverage the expertise of the Core staff. 3) To facilitate access to targeted and untargeted metabolomics/lipidomics determinations by the Columbia Irving Biomarker Laboratory and the newly expanded Metabolomics core. 4) To facilitate access to experts in bioinformatics, kinetic studies and pathway analyses required to appropriately interpret metabolomics/lipidomics determinations and stable isotopes kinetic studies. 5) To provide expert advice to investigators and their associates on analytical methods, experimental design, and data interpretation for state-of-the art phenotyping of diabetes. 6) To train junior investigators and post-doctoral fellows in practical skills in the Core methods and, if appropriate, transfer analytical methods to their own labs.7) To facilitate collaborations among the research base, advertise available resources, and develop and/or facilitate investigator access to new technologies, such as MS-based methods to analyze and quantify metabolites. TBAC analytic and consultative capabilities are closely articulated with the other DRC Cores to provide an integrated approach to quantitative metabolic analyses.