Systems Biology Core

系统生物学核心

• 批准号: 10271833
• 负责人: ROBERT POWERS
• 金额: $ 31.33万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10271833
• 关键词: Address; Adopted; Adoption; Applications Grants; Automation; Back; Biological Sciences; Client; Communication; Communications Personnel; Computer software; Conceptions; Core Facility; Data; Data Science; Dissemination and Implementation; E-learning; Education and Outreach; Educational workshop; Electron Microscopy; Ensure; Experimental Designs; Fee-for-Service Plans; Fees; Goals; Growth; Human Resources; Image; Industrialization; Information Dissemination; Infrastructure; Liquid substance; Mass Spectrum Analysis; Methods; Microscopy; Modeling; Molecular Analysis; Nebraska; Nuclear Magnetic Resonance; Outcome; Phase; Philosophy; Preparation; Procedures; Proteomics; Protocols documentation; Publications; RNA; Research; Research Personnel; Resources; Sampling; Series; Service delivery model; Services; Specialist; Structure; System; Systems Biology; Techniques; Technology; Time; Training; Universities; Vision; Wages; base; collaborative approach; complex biological systems; experience; imaging capabilities; improved; instructor; instrumentation; lipidomics; member; metabolomics; novel; organizational structure; outreach services; programs; research facility; response; software development; structural biology; success; training opportunity; training project

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of the Systems Biology Core (SBC) within the Nebraska Center for Integrated Biomolecular Communication (CIBC) is to serve as a sustainable resource that enables CIBC, the University of Nebraska-Lincoln (UNL), and other academic and industrial researchers to investigate complex biological systems. SBC provides a diverse set of OMICS (i.e., lipidomics, metabolomics, and proteomics), microscopy, and structural biology services, which afford distinct and invaluable views of an entire system and, accordingly, are critical to the needs of CIBC Project Leaders and other Center members. The successful application of microscopy, OMICs, and structural biology techniques are highly dependent on the expertise and experience of the investigator. Understanding the challenges these methods present to novice and new investigators has informed the overarching vision of SBC. SBC eschews the typical sample-based service model of a common facility and instead has adopted a personalized collaborative approach to enable the correct utilization of microscopy, OMICs, and structural biology methods by new investigators. In this regard, SBC provides assistance and training from project conception, experimental design, and sample preparation through implementation and dissemination. An important goal of SBC is enabling the long-term adoption of SBC technologies through personalized hands-on training and workshops—in other words, SBC personnel are collaborators and instructors. During Phase 1, SBC established its service pipeline and is providing efficient and high-quality data to CIBC personnel. SBC created the critical infrastructure for managing projects, samples, and data and the rapid dissemination of information to investigators. Building on the momentum and successes from Phase 1, the goal of SBC for Phase 2 is to grow and improve SBC service in response to the needs of CIBC Project Leaders and other Center members and to support CIBC in achieving its overall goals and long-term Center sustainability. This will be achieved by pursuing the following specific aims: 1) provide high-quality OMICs, microscopy, and structural biology assistance to CIBC projects to ensure investigators have adequate and timely access to core services, 2) enhance existing SBC OMICs techniques by expanding MS imaging, lipidomics, proteomics, and targeted metabolomics capabilities and improving the efficiency and throughput of the OMICS technology through automation, and 3) expand and enhance SBC scientific outreach services. The anticipated outcomes of SBC in Phase 2 are improved and expanded services that addresses the evolving needs of SBC clients while continuing to provide high quality and timely results. SBC will also strive to increase the throughput and efficiency of the service pipeline, expand the SBC user-base, and provide additional training opportunities that enables the adaption of OMICS, microscopy, and structural biology by non-experts.

项目摘要/摘要 内布拉斯加州综合中心内系统生物学核心（SBC）的广泛长期目标 生物分子通信（CIBC）将作为可持续资源，使CIBC，大学 内布拉斯加州 - 林肯（UNL）以及其他学术和工业研究人员，以调查复杂的生物学 系统。 SBC提供了一组Omics（即脂肪组学，代谢组学和蛋白质组学），显微镜，显微镜， 以及结构生物学服务，这些服务具有整个系统的独特和宝贵的观点，因此 对于CIBC项目领导者和其他中心成员的需求至关重要。成功应用 显微镜，OMIC和结构生物学技术高度依赖于 调查员。了解这些方法对新颖调查人员的挑战 告知SBC的总体愿景。 SBC避免了常见的典型基于样本的服务模型 设施并采用了个性化的协作方法来正确利用 新研究者的显微镜，奥理和结构生物学方法。在这方面，SBC提供 通过项目概念，实验设计和样本准备的帮助和培训 实施和传播。 SBC的重要目标是使SBC长期采用 通过个性化的动手培训和讲习班技术 - 换句话说，SBC人员是 合作者和讲师。在第1阶段，SBC建立了其服务管道，并提供高效 CIBC人员的高质量数据。 SBC创建了用于管理项目，样本和的关键基础架构 数据和信息快速传播给研究者。基于势头和成功 第1阶段，SBC对于第2阶段的目标是根据CIBC的需求增长和改善SBC服务 项目领导者和其他中心成员，并支持CIBC实现其整体目标和长期目标 中心可持续性。这将通过追求以下特定目的来实现：1）提供高质量 对CIBC项目的OMICS，显微镜和结构生物学援助，以确保调查人员足够 并及时访问核心服务，2）通过扩展MS成像，增强现有的SBC OMICS技术， 脂肪组学，蛋白质组学和靶向代谢组学能力，并提高效率和吞吐量 通过自动化的OMICS技术，以及3）扩展和增强SBC科学外展服务。 第2阶段SBC的预期结果得到了改进和扩展的服务，以满足不断发展的需求 SBC客户的同时继续提供高质量和及时的结果。 SBC也将努力增加 服务管道的吞吐量和效率，扩展SBC用户群，并提供额外的培训 可以通过非专家对OMICS，显微镜和结构生物学的适应机会。