Systems Biology Core

摘要 内布拉斯加州综合生物分子通讯中心（CIBC）的研究人员将调查 复杂的生物系统。因此，这些研究人员希望广泛利用质量 光谱（MS）、显微镜和核磁共振（NMR）光谱。这些能力在 系统生物学目前可通过三个现有的内布拉斯加大学林肯（UNL）核心 设施然而，这些独立的、不同的核心给研究人员带来了实际和逻辑上的障碍 需要有效地将系统生物学纳入他们的项目。现有的核心结构需要一个 研究者通过与每个单独的核心互动和设计重复的方案来重复工作。在 此外，每个核心都专注于将其特定的专业知识和技术应用于一个问题，而不是 对所有替代品的广泛、协同观点。一个系统生物学的核心设施， 单一组织结构下的工具、方法和专业知识将有助于确保UNL 生物医学研究人员接受系统生物学技术的支持和培训， 研究需要。因此，系统生物学核心（SBC）设施的建立将解决具体的， CIBC调查员的关键要求。SBC设施的长期目标是创建一个可持续的系统， 生物设施，满足CIBC成员，UNL教师和更大的组学和显微镜的需要 科学界。SBC设施的短期目标是促进系统生物学的适应 CIBC和UNL调查人员的技术。SBC将通过培训和教育来实现其目标 研究人员在系统生物学方法和不断扩大和加强SBC的能力， 仪器、技术和能力。通过SBC设施，CIBC成员将有例行的 与熟练的组学和显微镜专家互动，他们可以协助研究设计、样本制备， 以及对结果数据的收集、分析、解释和建模。SBC人员还将与 与中心的数据管理和分析核心，以促进CIBC项目，需要数据挖掘， 存档、统计分析、功能和预测分析以及计算建模。因此，SBC 该设施将加强对CIBC调查人员和UNL教师至关重要的领域的基础设施 更广泛地说。建立SBC核心需要实现三个具体目标：1）聘请董事 谁将监督核心; 2）聘请两名新的核磁共振和MS代谢组学专家为研究人员提供建议 研究设计、样品制备和数据采集、分析和解释;以及3）高效 为CIBC项目提供组学和显微镜协助，以确保研究人员有足够的，及时的 获得核心服务。作为一个集中的设施，SBC将使中心的研究人员能够有效地 将系统生物学方法纳入其项目，从而加强现有的基础设施， 提供系统生物学仪器和方法的简化访问。

ABSTRACT Researchers in the Nebraska Center for Integrated Biomolecular Communication (CIBC) will be investigating complex biological systems. As a result, these researchers expect to make extensive use of mass spectroscopy (MS), microscopy, and nuclear magnetic resonance (NMR) spectroscopy. These capabilities in systems biology are currently available through three existing University of Nebraska-Lincoln (UNL) core facilities. However, these separate, distinct cores present practical and logistic impediments to researchers needing to effectively incorporate systems biology into their projects. The existing core structure requires an investigator to duplicate effort by interacting with each separate core and devising repetitive protocols. In addition, each individual core focuses on applying its specific expertise and technology to a problem instead of a broad, synergistic view of all alternatives. A systems biology core facility that incorporates all systems biology instrumentation, methodologies, and expertise under a single organizational structure will help ensure that UNL biomedical investigators receive support and training in the systems biology techniques that best fit their research needs. Thus, the establishment of the Systems Biology Core (SBC) facility will address the specific, critical requirements of CIBC investigators. The SBC facility's long-term goal is to create a sustainable systems biology facility that meets the omics and microscopy needs of CIBC members, UNL faculty, and the larger scientific community. The SBC facility's short-term goal is to facilitate the adaption of systems biology technologies by CIBC and UNL investigators. The SBC will accomplish its goals by training and educating investigators in systems biology methodology and by continually expanding and enhancing SBC capabilities, instrumentation, technologies, and capacity. Through the SBC facility, CIBC members will have routine interactions with skilled omics and microscopy experts who can assist with study design, sample preparation, and the collection, analysis, interpretation, and modeling of resulting data. SBC personnel will also collaborate with the Center's Data Management and Analysis Core to facilitate CIBC projects that require data mining and archiving, statistical analysis, functional and predictive analyses, and computational modeling. Thus, the SBC facility will enhance infrastructure in an area of critical importance to CIBC investigators and to UNL faculty more broadly. Establishing the SBC core will require accomplishing three specific aims: 1) Engage a director who will oversee the core; 2) Hire two new staff experts in NMR and MS metabolomics to advise investigators on study design, sample preparation, and data acquisition, analysis, and interpretation; and 3) Efficiently provide omics and microscopy assistance to CIBC projects to ensure investigators have adequate, timely access to core services. As a centralized facility, the SBC will enable Center researchers to effectively integrate systems biology methodologies into their projects, thus enhancing existing infrastructure and providing streamlined access to systems biology instrumentation and methodology.