Systems Biology Core

系统生物学核心

• 批准号: 10488658
• 负责人: ROBERT POWERS
• 金额: $ 46.45万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10488658
• 关键词: Address; Adopted; Adoption; Applications Grants; Automation; Back; Biological Sciences; Client; Communication; Communications Personnel; Computer software; Conceptions; Core Facility; Data; Data Science; Dissemination and Implementation; E-learning; Education and Outreach; Educational workshop; Electron Microscopy; Ensure; Experimental Designs; Fee-for-Service Plans; Fees; Goals; Growth; Human Resources; Industrialization; Information Dissemination; Infrastructure; Liquid substance; Mass Spectrum Analysis; Methods; Microscopy; Modeling; Molecular Analysis; Nebraska; Nuclear Magnetic Resonance; Outcome; Phase; Philosophy; Preparation; Procedures; Proteomics; Protocols documentation; Publications; RNA; Research; Research Personnel; Sampling; Series; Service delivery model; Services; Specialist; Structure; System; Systems Biology; Techniques; Technology; Time; Training; Universities; Vision; Wages; base; collaborative approach; complex biological systems; experience; imaging capabilities; improved; instructor; instrumentation; lipidomics; mass spectrometric imaging; member; metabolomics; novel; organizational structure; outreach services; programs; research facility; response; software development; structural biology; success; sustainable resource; training opportunity; training project

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of the Systems Biology Core (SBC) within the Nebraska Center for Integrated Biomolecular Communication (CIBC) is to serve as a sustainable resource that enables CIBC, the University of Nebraska-Lincoln (UNL), and other academic and industrial researchers to investigate complex biological systems. SBC provides a diverse set of OMICS (i.e., lipidomics, metabolomics, and proteomics), microscopy, and structural biology services, which afford distinct and invaluable views of an entire system and, accordingly, are critical to the needs of CIBC Project Leaders and other Center members. The successful application of microscopy, OMICs, and structural biology techniques are highly dependent on the expertise and experience of the investigator. Understanding the challenges these methods present to novice and new investigators has informed the overarching vision of SBC. SBC eschews the typical sample-based service model of a common facility and instead has adopted a personalized collaborative approach to enable the correct utilization of microscopy, OMICs, and structural biology methods by new investigators. In this regard, SBC provides assistance and training from project conception, experimental design, and sample preparation through implementation and dissemination. An important goal of SBC is enabling the long-term adoption of SBC technologies through personalized hands-on training and workshops—in other words, SBC personnel are collaborators and instructors. During Phase 1, SBC established its service pipeline and is providing efficient and high-quality data to CIBC personnel. SBC created the critical infrastructure for managing projects, samples, and data and the rapid dissemination of information to investigators. Building on the momentum and successes from Phase 1, the goal of SBC for Phase 2 is to grow and improve SBC service in response to the needs of CIBC Project Leaders and other Center members and to support CIBC in achieving its overall goals and long-term Center sustainability. This will be achieved by pursuing the following specific aims: 1) provide high-quality OMICs, microscopy, and structural biology assistance to CIBC projects to ensure investigators have adequate and timely access to core services, 2) enhance existing SBC OMICs techniques by expanding MS imaging, lipidomics, proteomics, and targeted metabolomics capabilities and improving the efficiency and throughput of the OMICS technology through automation, and 3) expand and enhance SBC scientific outreach services. The anticipated outcomes of SBC in Phase 2 are improved and expanded services that addresses the evolving needs of SBC clients while continuing to provide high quality and timely results. SBC will also strive to increase the throughput and efficiency of the service pipeline, expand the SBC user-base, and provide additional training opportunities that enables the adaption of OMICS, microscopy, and structural biology by non-experts.

项目总结/摘要 系统生物学核心（SBC）在内布拉斯加州综合中心的广泛，长期目标 生物分子通信（CIBC）是作为一个可持续的资源，使CIBC，大学 内布拉斯加州林肯大学（UNL）和其他学术和工业研究人员研究复杂的生物 系统. SBC提供了一组不同的OMICS（即，脂质组学、代谢组学和蛋白质组学），显微术， 和结构生物学服务，提供整个系统的独特和宝贵的意见，因此， 对CIBC项目负责人和其他中心成员的需求至关重要。的成功应用 显微镜，OMIC和结构生物学技术高度依赖于专业知识和经验， 调查员了解这些方法对新手和新调查人员提出的挑战， 为SBC的总体愿景提供了信息。SBC避开了通用的基于示例的典型服务模型， 设施，而是采取了个性化的协作方法，使正确利用 显微镜、OMIC和结构生物学方法。在这方面，SBC提供 从项目构思、实验设计和样品制备到 执行和传播。SBC的一个重要目标是实现SBC的长期采用 技术，通过个性化的实践培训和研讨会-换句话说，SBC人员 合作者和指导者。在第一阶段，SBC建立了服务管道，并提供高效和 向CIBC人员提供高质量的数据。SBC创建了用于管理项目、样本和 数据和迅速向调查人员传播信息。在发展势头和成功的基础上， 第一阶段，SBC第二阶段的目标是根据CIBC的需求发展和改进SBC服务 项目负责人和其他中心成员，并支持CIBC实现其总体目标和长期目标 中心的可持续性。这将通过追求以下具体目标来实现：1）提供高质量的 为CIBC项目提供OMIC、显微镜和结构生物学援助，以确保研究人员有足够的 和及时获得核心服务，2）通过扩展MS成像增强现有SBC OMIC技术， 脂质组学、蛋白质组学和靶向代谢组学能力，并提高 OMICS技术，以及3）扩大和加强SBC科学推广服务。的 SBC在第二阶段的预期成果是改进和扩展服务，以满足不断变化的需求 同时继续提供高质量和及时的结果。SBC还将努力增加 提高服务管道的吞吐量和效率，扩大SBC用户群，并提供额外的培训 机会，使适应OMICS，显微镜和结构生物学的非专家。