Systems Biology Core

项目摘要/摘要 内布拉斯加州综合研究中心系统生物学核心(SBC)的广泛、长期目标 生物分子通信(CIBC)将作为一种可持续的资源，使CIBC能够 内布拉斯加州-林肯(UNL)和其他学术和工业研究人员研究复杂的生物学 系统。SBC提供了一组不同的OMICS(即，脂质组学、代谢组学和蛋白质组学)、显微镜、 和结构生物学服务，它们提供了整个系统的独特和宝贵的观点，因此， 对CIBC项目负责人和其他中心成员的需求至关重要。成功地应用了 显微镜、组学和结构生物学技术高度依赖于 调查员。了解这些方法给新手和新研究者带来的挑战 为SBC的总体愿景提供了信息。SBC避开了典型的基于样本的服务模型 设施，转而采用个性化协作方法，以实现正确利用 新研究人员的显微镜、组学和结构生物学方法。在这方面，SBC提供了 项目构思、实验设计和样品准备方面的协助和培训 实施和传播。SBC的一个重要目标是使SBC能够长期采用 通过个性化的实践培训和研讨会-换言之，SBC人员 合作者和讲师。在第一阶段，SBC建立了其服务渠道，并正在提供高效和 为CIBC人员提供高质量的数据。SBC创建了用于管理项目、样本和 数据和向调查人员迅速传播信息。在以下方面的势头和成功的基础上 第一阶段，第二阶段SBC的目标是发展和改进SBC服务，以响应CIBC的需求 并支持加拿大帝国商业银行实现其总体目标和长远目标 中心可持续发展。这将通过追求以下具体目标来实现：1)提供高质量 组学、显微镜和结构生物学对CIBC项目的援助，以确保调查人员有足够的 并及时获得核心服务，2)通过扩展MS成像来增强现有的SBC组学技术， 脂质组学、蛋白质组学和靶向代谢组学能力，并提高效率和吞吐量 OMICS技术通过自动化，以及3)扩大和加强SBC科学外展服务。这个 SBC在第二阶段的预期结果是改进和扩展服务，以满足不断变化的需求 在继续提供高质量和及时的结果的同时，SBC的客户。SBC还将努力增加 提高服务渠道的吞吐量和效率，扩大SBC用户群，并提供额外培训 使非专家能够适应OMICS、显微镜和结构生物学的机会。

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of the Systems Biology Core (SBC) within the Nebraska Center for Integrated Biomolecular Communication (CIBC) is to serve as a sustainable resource that enables CIBC, the University of Nebraska-Lincoln (UNL), and other academic and industrial researchers to investigate complex biological systems. SBC provides a diverse set of OMICS (i.e., lipidomics, metabolomics, and proteomics), microscopy, and structural biology services, which afford distinct and invaluable views of an entire system and, accordingly, are critical to the needs of CIBC Project Leaders and other Center members. The successful application of microscopy, OMICs, and structural biology techniques are highly dependent on the expertise and experience of the investigator. Understanding the challenges these methods present to novice and new investigators has informed the overarching vision of SBC. SBC eschews the typical sample-based service model of a common facility and instead has adopted a personalized collaborative approach to enable the correct utilization of microscopy, OMICs, and structural biology methods by new investigators. In this regard, SBC provides assistance and training from project conception, experimental design, and sample preparation through implementation and dissemination. An important goal of SBC is enabling the long-term adoption of SBC technologies through personalized hands-on training and workshops—in other words, SBC personnel are collaborators and instructors. During Phase 1, SBC established its service pipeline and is providing efficient and high-quality data to CIBC personnel. SBC created the critical infrastructure for managing projects, samples, and data and the rapid dissemination of information to investigators. Building on the momentum and successes from Phase 1, the goal of SBC for Phase 2 is to grow and improve SBC service in response to the needs of CIBC Project Leaders and other Center members and to support CIBC in achieving its overall goals and long-term Center sustainability. This will be achieved by pursuing the following specific aims: 1) provide high-quality OMICs, microscopy, and structural biology assistance to CIBC projects to ensure investigators have adequate and timely access to core services, 2) enhance existing SBC OMICs techniques by expanding MS imaging, lipidomics, proteomics, and targeted metabolomics capabilities and improving the efficiency and throughput of the OMICS technology through automation, and 3) expand and enhance SBC scientific outreach services. The anticipated outcomes of SBC in Phase 2 are improved and expanded services that addresses the evolving needs of SBC clients while continuing to provide high quality and timely results. SBC will also strive to increase the throughput and efficiency of the service pipeline, expand the SBC user-base, and provide additional training opportunities that enables the adaption of OMICS, microscopy, and structural biology by non-experts.