The Biophysics Collaborative Access Team

生物物理学协作访问团队

• 批准号: 10561672
• 负责人: THOMAS C IRVING
• 金额: $ 121.67万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10561672
• 关键词: Address; Advisory Committees; Aging; Amyloid; Automation; Biochemical; Biocompatible Materials; Biological; Biology; Biophysics; Biotechnology; Cardiomyopathies; Cell physiology; Cells; Charge; Collaborations; Communities; Complex; Computer software; Connective Tissue; Data; Data Analyses; Dedications; Development; Disease model; Drug Design; Educational workshop; Ensure; Equilibrium; Equipment; Evolution; Fiber; Funding; Grant; Human Pathology; Illinois; Image; Individual; Infrastructure; Instruction; Laboratories; Leadership; Ligands; Light; Maintenance; Mission; Modification; Molecular; Molecular Biology; Molecular Sieve Chromatography; Muscle; Myocardium; National Center for Research Resources; National Institute of General Medical Sciences; Neoplasm Metastasis; Noise; Nucleic Acid Folding; Nucleic Acids; Pathology; Persons; Photons; Physics; Physiological; Policies; Principal Investigator; Productivity; Proteins; Publications; Radiation induced damage; Recruitment Activity; Research; Research Personnel; Research Project Grants; Research Technics; Resources; Sampling; Scanning; Schedule; Science; Shapes; Signal Transduction; Site; Skeletal Muscle; Source; Structure; Students; Synchrotrons; System; Technology; Time; Tissue Sample; Training; Training Activity; Traumatic Brain Injury; United States National Institutes of Health; Update; Visit; X ray diffraction analysis; base; beamline; biological systems; biophysical properties; community organizations; computerized data processing; data acquisition; data quality; experience; experimental study; fibrous protein; improved; inherited cardiomyopathy; instrumentation; interest; macromolecule; operation; outreach; prevent; professor; programs; protein structure; repaired; structural biology; symposium; synchrotron radiation; tool; training opportunity; user-friendly; web site

The mission of the Biophysics Collaborative Access Team (BioCAT) is to provide user access to a state-of-the- art synchrotron beamline (18ID) for diffraction and scattering of non-crystalline biological materials at the Advanced Photon Source, Argonne National Lab. Continued evolution of the beamline since first light in 1997 has resulted in a unique in the USA fiber diffraction program, and a world-class Bio-SAXS program with unique capabilities both in the equilibrium and time-resolved realms. The use of size exclusion chromatography in line with the SAXS camera has ensured unprecedentedly high data quality even with biochemically challenging samples otherwise thought to be intractable for SAXS. The recent incorporation of MALS and DLS in line with SEC allows additional biophysical characterization that can be invaluable in interpreting SAXS data from difficult systems. Our new coflow sheath flow sample cell has revolutionized SAXS data acquisition by preventing radiation damage, enhancing efficiency accompanied by significant increases in signal to noise. BioCAT maintains specialized apparatuses for combined time-resolved X-ray diffraction and physiological studies of cardiac and skeletal muscle. These studies have extended in scope from basic biophysical problems to address new disease models including cardiomyopathies. A traditional strength is fiber diffraction of general fibrous protein systems that has recently expanded into scanning diffraction imaging of fibrous protein structures in complex tissue samples. A major strength of the resource is the experienced and dedicated scientific staff who assist users seven days a week in data acquisition, processing, analysis and interpretation. BioCAT is directed by Dr. Thomas Irving, who has more than 20 years of experience overseeing synchrotron facilities for non-crystalline diffraction and scattering. Dr. Jesse Hopkins oversaw installation of the new coflow SAXS sample cell, established new user-friendly GUI based data acquisition interfaces, and provide advanced tools to interpret SEC-SAXS data. Dr. Hopkins develops and maintains the BioXTAS RAW SAXS data processing and analysis software package and will extend it to allow increased automation to optimize productivity while minimizing on-site effort for users. Dr. Srinivas Chakravarthy BioCAT’s resident biochemist coordinates the SAXS user program, assists users with conducting experiments on site, and also maintains the biochemical infrastructure. Dr. Weikang Ma is an expert in fiber diffraction of muscle and is responsible for providing comprehensive support to all our fiber diffraction users. All four of these individuals are involved in training and dissemination activities, including scientific publications, presentations at conferences, organization of BioCAT workshops and participating in community organized workshops. BioCAT has an updated website with a comprehensive set of instructions for all aspects of the resource that are sufficient for a user to plan and execute successful experiments at the beamline. Together the SAXS and Fiber Diffraction user programs host 70-90 user groups per year and consistently produce 30-40 publications per year.

生物物理学协作访问团队（BioCAT）的使命是为用户提供访问最新状态的 先进的同步加速器光束线（18 ID），用于非晶体生物材料的衍射和散射， 先进光子源，阿贡国家实验室。自1997年首次亮相以来，光束线不断发展 已经导致了在美国独特的纤维衍射程序，和一个世界级的生物SAXS程序，具有独特的 平衡和时间分辨领域的能力。使用尺寸排阻色谱在线 SAXS相机确保了前所未有的高数据质量，即使在具有生物化学挑战性的环境中， 否则被认为难以进行SAXS的样品。最近根据《联合国宪章》合并了MALS和DLS， SEC允许额外的生物物理表征，这在解释SAXS数据时是非常宝贵的， 困难的系统。我们的新型coflow鞘流样品池彻底改变了SAXS数据采集， 防止辐射损伤，提高效率，同时显著增加信噪比。 BioCAT拥有专门的设备，用于结合时间分辨X射线衍射和生理 心脏和骨骼肌的研究。这些研究的范围从基本的生物物理问题 以解决包括心肌病在内的新疾病模型。一种传统的强度是纤维衍射的一般 纤维状蛋白质系统最近已扩展到纤维状蛋白质的扫描衍射成像 复杂组织样本中的结构。一个主要的优势资源是经验丰富， 科学工作人员每周七天协助用户获取、处理、分析和解释数据。 BioCAT由托马斯欧文博士指导，他有20多年的监督同步加速器的经验 用于非晶体衍射和散射的设备。Jesse霍普金斯博士监督新coflow的安装 SAXS样品池，建立了新的用户友好的基于GUI的数据采集界面，并提供先进的 解释SEC-SAXS数据的工具。霍普金斯博士开发和维护BioXTAS RAW SAXS数据 处理和分析软件包，并将对其进行扩展，以提高自动化程度， 生产力，同时最大限度地减少用户的现场工作。Dr. Srinivas Chakravarthy BioCAT的常驻生物化学家 协调SAXS用户程序，协助用户进行现场实验，并维护 生化基础设施。马伟康博士是肌肉纤维衍射方面的专家， 为所有光纤衍射用户提供全面的支持。这四个人都参与了 培训和传播活动，包括科学出版物、会议介绍、 组织BioCAT研讨会并参加社区组织的研讨会。BioCAT有一个 更新的网站与一套全面的指示，为所有方面的资源，是足够的， 用户可以在光束线上计划和执行成功的实验。小角X射线散射和光纤衍射 用户方案每年接待70-90个用户团体，每年持续出版30-40份出版物。