The Biophysics Collaborative Access Team Fiber Diffraction Core)

生物物理学协作访问团队光纤衍射核心）

• 批准号: 10561677
• 负责人: THOMAS C IRVING
• 金额: $ 44.82万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10561677
• 关键词: 20 year old; Acceleration; Address; Amyloid; Animal Model; Archives; Area; Biocompatible Materials; Biological; Biophysics; Collaborations; Communities; Complex; Computer software; Connective Tissue; Crystallography; Data; Data Analyses; Diagnostic; Ensure; Equipment; Experimental Designs; Feedback; Fiber; Funding; Hour; Image; Individual; Infrastructure; International; Maintenance; Metadata; Modality; Modeling; Modernization; Molecular Structure; Muscle; Muscle Contraction; Muscle Proteins; Myocardium; Neoplasm Metastasis; Network Infrastructure; Neurodegenerative Disorders; Peer Review; Performance; Procedures; Publications; Quality Control; Recording of previous events; Regulation; Reproducibility; Resource Development; Resources; Roentgen Rays; Sampling; Scanning; Schedule; Seminal; Site; Skeletal Muscle; Skin; Source; Striated Muscles; Structure; Synchrotrons; System; Technology; Time; Tissues; Training; Transgenic Animals; Traumatic Brain Injury; United States National Institutes of Health; Visit; Work; beamline; brain tissue; computer infrastructure; data acquisition; data analysis pipeline; data infrastructure; data management; data quality; data quality/integrity; data reduction; detector; experience; experimental study; fibrous protein; human disease; imaging study; improved; inherited cardiomyopathy; insight; instrument; instrumentation; muscular system; operation; programs; simulation; software systems

The objective of the Fiber Diffraction Technology Operations Core at BioCAT is to provide the biomedical community access to optimized facilities for fiber diffraction of muscle and fibrous protein systems that are unique in the USA and competitive with all other facilities worldwide. The muscle diffraction program at BioCAT is renowned for its history of addressing important questions regarding the biophysics of muscle contraction and its regulation. More recently, emphasis has shifted to more directly biomedically relevant problems with muscle from transgenic animal models for human disease, including inherited cardiomyopathies. BioCAT is also known for the seminal fiber crystallography studies on the structure of collagenous and amyloid systems. In recent years, there has been a shift from studying individual fibrous protein samples towards spatially- resolved scanning diffraction imaging studies that examine the relationships between molecular structure and tissue organization in complex biological tissues such as brain and connective tissue in order to address maladies such as traumatic brain injury, neurodegenerative diseases and cancer metastasis. Our staff are internationally recognized experts in fiber diffraction able to provide expertise in all aspects of a project from experimental design, execution of the experiments, through to data analysis and interpretation. Staff work closely throughout the experiments with first time users and in the context of formal collaborations involving complicated experiments. For routine experiments by experienced users, staff provide onsite training, 24/7 on- call support and are available for help both before and after scheduled experiments. We present plans to adapt the BioCAT beamline 18ID to exploit the outstanding opportunities presented by the APS upgrade (APS-U). This includes plans to upgrade the main area detector to a state-of-the-art EIGER2 XE 9M pixel array detector with necessary upgrades to networking and computational infrastructure. The 20 years old beamline will be upgraded with modern control systems and diagnostics with continuing routine maintenance and contingency plans to ensure reliable operations. An ultra-high flux wide bandpass X-ray monochromator will be installed in order to maintain our competitive edge with other facilities nationally and worldwide to the benefit of all experimental modalities. User experience, quality and integrity of data will be enhanced by continuing improvements to the BioCAT developed experimental control hardware/software system, BioCon. The MuscleX data reduction and analysis package, also developed at BioCAT, for muscle diffraction, fiber crystallography, and scanning diffraction imaging will be enhanced and integrated into data analysis pipelines to provide real time feedback to users during experiments as well as greatly accelerate time to publication. BioCAT will provide training and access to multi-scale simulations for interpreting dynamic muscle diffraction data. Procedures and infrastructure will be developed and deployed for robust data and meta-data curation and archiving.

BioCAT的光纤衍射技术运营核心的目标是提供生物医学 社区可以使用优化的肌肉和纤维蛋白系统纤维衍射设施， 在美国是独一无二的，与全球所有其他设施竞争。BioCAT的肌肉衍射程序 因其解决有关肌肉收缩的生物物理学重要问题的历史而闻名 及其监管。最近，重点已经转移到更直接的生物医学相关问题， 肌肉从转基因动物模型的人类疾病，包括遗传性心肌病。BioCAT是 也因对胶原和淀粉样蛋白系统结构的精液纤维晶体学研究而闻名。 近年来，已经从研究单个纤维蛋白样品转向空间- 解析扫描衍射成像研究，检查分子结构与 复杂生物组织如脑和结缔组织中的组织组织， 如创伤性脑损伤、神经退行性疾病和癌症转移等疾病。我们的工作人员是 国际公认的纤维衍射专家，能够在项目的各个方面提供专业知识， 实验设计，实验的执行，通过数据分析和解释。工作人员工作 在与第一次使用者的实验中，以及在正式合作的背景下， 复杂的实验对于经验丰富的用户进行的常规实验，工作人员提供现场培训，24/7， 在预定的实验之前和之后都可以获得帮助。我们提出计划， BioCAT光束线18 ID，以利用APS升级（APS-U）带来的出色机会。 这包括计划将主区域探测器升级为最先进的EIGER 2 XE 9 M像素阵列探测器 并对网络和计算基础设施进行必要的升级。20岁的光束线将是 升级了现代化的控制系统和诊断系统，并持续进行日常维护和应急 以确保可靠的运营。一台超高通量宽带通X射线单色仪将安装在 为了保持我们的竞争优势与其他设施国内和世界各地的利益， 实验模式用户体验、数据质量和完整性将通过继续 改进BioCAT开发的实验控制硬件/软件系统BioCon。的 MuscleX数据简化和分析包，也是在BioCAT开发的，用于肌肉衍射，纤维 晶体学和扫描衍射成像将得到增强，并集成到数据分析管道中 以在实验期间向用户提供真实的时间反馈，并大大加快出版时间。 BioCAT将提供培训和多尺度模拟，以解释动态肌肉衍射 数据将制定和部署程序和基础设施，以管理健全的数据和元数据 和归档。