The Biophysics Collaborative Access Team Fiber Diffraction Core)

BioCAT的光纤衍射技术运营核心的目标是提供生物医学 社区可获得用于肌肉和纤维蛋白系统的纤维衍射的优化设施 在美国是独一无二的，与世界各地的所有其他设施相比具有竞争力。BioCAT的肌肉衍射计划 以解决有关肌肉收缩的生物物理学的重要问题而闻名 以及它的规定。最近，重点已经转移到与生物医学更直接相关的问题上 来自人类疾病转基因动物模型的肌肉，包括遗传性心肌病。BioCAT是 也以精液纤维结晶学研究胶原和淀粉样蛋白系统的结构而闻名。 近年来，研究单个纤维蛋白样本的研究已经转向空间- 分辨率扫描衍射成像研究，检查分子结构和 在脑和结缔组织等复杂生物组织中的组织结构，以解决 疾病，如创伤性脑损伤、神经退行性疾病和癌症转移。我们的工作人员是 国际公认的光纤衍射专家能够为项目的所有方面提供专业知识 实验设计、实验执行，一直到数据的分析和解释。员工工作 在与第一次用户的实验中以及在正式协作的背景下，包括 复杂的实验。对于经验丰富的用户的常规实验，工作人员提供全天候现场培训- 拨打支持电话，并在预定的实验前后均可获得帮助。我们提出了适应环境变化的计划 BioCAT光束线18ID，以利用APS升级(APS-U)带来的巨大机遇。 这包括计划将主要区域探测器升级为最先进的EIGER2 XE 9M像素阵列探测器 对网络和计算基础设施进行必要的升级。已有20年历史的光束线将是 升级了现代控制系统和诊断系统，并持续进行例行维护和应急 计划确保可靠的运营。将安装一台超高通量宽带X射线单色仪 为了保持我们与国内和世界其他设施的竞争优势，造福于所有人 实验模式。用户体验、数据质量和完整性将通过继续 对BioCAT开发的实验控制硬件/软件系统Biocon进行了改进。这个 MuscleX数据简化和分析包，也是BioCAT开发的，用于肌肉衍射、纤维 结晶学和扫描衍射成像将得到加强，并整合到数据分析管道中 在实验期间向用户提供实时反馈，并极大地加快出版时间。 BioCAT将提供培训和使用多尺度模拟来解释动态肌肉衍射 数据。将制定和部署程序和基础设施，以进行可靠的数据和元数据管理 和存档。

The objective of the Fiber Diffraction Technology Operations Core at BioCAT is to provide the biomedical community access to optimized facilities for fiber diffraction of muscle and fibrous protein systems that are unique in the USA and competitive with all other facilities worldwide. The muscle diffraction program at BioCAT is renowned for its history of addressing important questions regarding the biophysics of muscle contraction and its regulation. More recently, emphasis has shifted to more directly biomedically relevant problems with muscle from transgenic animal models for human disease, including inherited cardiomyopathies. BioCAT is also known for the seminal fiber crystallography studies on the structure of collagenous and amyloid systems. In recent years, there has been a shift from studying individual fibrous protein samples towards spatially- resolved scanning diffraction imaging studies that examine the relationships between molecular structure and tissue organization in complex biological tissues such as brain and connective tissue in order to address maladies such as traumatic brain injury, neurodegenerative diseases and cancer metastasis. Our staff are internationally recognized experts in fiber diffraction able to provide expertise in all aspects of a project from experimental design, execution of the experiments, through to data analysis and interpretation. Staff work closely throughout the experiments with first time users and in the context of formal collaborations involving complicated experiments. For routine experiments by experienced users, staff provide onsite training, 24/7 on- call support and are available for help both before and after scheduled experiments. We present plans to adapt the BioCAT beamline 18ID to exploit the outstanding opportunities presented by the APS upgrade (APS-U). This includes plans to upgrade the main area detector to a state-of-the-art EIGER2 XE 9M pixel array detector with necessary upgrades to networking and computational infrastructure. The 20 years old beamline will be upgraded with modern control systems and diagnostics with continuing routine maintenance and contingency plans to ensure reliable operations. An ultra-high flux wide bandpass X-ray monochromator will be installed in order to maintain our competitive edge with other facilities nationally and worldwide to the benefit of all experimental modalities. User experience, quality and integrity of data will be enhanced by continuing improvements to the BioCAT developed experimental control hardware/software system, BioCon. The MuscleX data reduction and analysis package, also developed at BioCAT, for muscle diffraction, fiber crystallography, and scanning diffraction imaging will be enhanced and integrated into data analysis pipelines to provide real time feedback to users during experiments as well as greatly accelerate time to publication. BioCAT will provide training and access to multi-scale simulations for interpreting dynamic muscle diffraction data. Procedures and infrastructure will be developed and deployed for robust data and meta-data curation and archiving.