The Biophysics Collaborative Access Team (SAXS Core)

BioCAT的SAXS计划的主要目标是为生物医学社区提供访问 小角x射线散射仪，并保持最先进的。BioCAT培养了一位用户 该计划具有丰富的富有成效的合作历史，其中包括一系列不同的生物医学兴趣。用户 为现场用户提供全天候支持，BioCAT提供远程协作 光束线人员的参与度。有两大类实验在 SAXS计划：平衡和时间分辨SAXS。高效液相色谱仪(LC-SAXS)组成 &gt；90%的平衡SAXS计划。LC-SAXS与同向流动样品池结合，基于 首先由澳大利亚SAXS光束线采用的设计，通过以下方式简化了BioCAT的SAXS数据采集 消除了常见的问题，如样品的异质性和辐射损伤导致的毛细管结垢。 在线多角度光散射(MALS)和动态光散射(DLS)探测器的引入 进一步提高了使用我们的平衡SAXS仪器获取的数据的范围和质量。作为该计划的一部分 时间分辨SAXS计划，有三个不同的选项可供选择，以迎合从~80开始的时间制度 微秒到几秒。建议使用停流混合器或层流混合器 访问时间范围在毫秒到几秒之间，具体取决于样本量 可用的和正在研究的系统。混乱流混合器用于访问较早的时间段(~80微米S 至~90毫秒)。优化的流体输送系统、协调的触发机制和基于图形用户界面的用户友好 内部开发的软件工具现在已经使连续流动时间分辨SAXS仪器在 BioCAT是世界上同类产品之一，向普通用户社区开放。BioCAT还维护了广泛的 使用开源数据分析程序BioXTAS RAW。维护和升级计划包括改进 液相色谱系统、数据分析软件和微流控混合器，以及升级 计算、数据存储能力、网络基础设施和探测器。我们将全面优化 利用APS-U项目将提供的世界级光束的仪器。最后但不是 至少我们将继续提高SAXS数据采集的自动化程度，以便使用户成为 尽可能地独立。

The primary objective of the SAXS program at BioCAT is to provide the biomedical community access to the small angle x-ray scattering instrument and to maintain the state-of-the-art. BioCAT has nurtured a user program with a rich history of productive collaborations that include a diverse set of biomedical interests. User support is provided 24/7 for onsite users, and BioCAT provides remote collaborations that involve a high degree of beamline personnel participation. There are two broad categories of experiments performed under the SAXS program: equilibrium and time-resolved SAXS. Liquid chromatography SAXS (LC-SAXS) constitutes > 90% of the equilibrium SAXS program. LC-SAXS in combination with a coflow sample cell, based on a design first adopted by the Australian SAXS beamline, has streamlined SAXS data acquisition at BioCAT by nullifying common concerns such as sample heterogeneity and radiation damage induced capillary fouling. Introduction of in-line multi-angle light scattering (MALS) and dynamic light scattering (DLS) detectors has further enhanced the scope and quality of data acquired using our equilibrium SAXS instrument. As part of the time-resolved SAXS program, three different options are available which cater to time regimes from ~80 microseconds to several seconds. The stopped-flow mixer or a laminar-flow mixer are recommended for accessing time regimes between a millisecond and a few seconds, depending on the amount of sample available and the system being studied. The chaotic flow mixer is used to access earlier time regimes (~ 80µs to ~ 90 ms). Optimized fluid delivery systems, coordinated triggering mechanisms, and GUI based user-friendly software tools developed in house have now made the continuous flow time-resolved SAXS instrument at BioCAT one of its kind in the world and open to the general user community. BioCAT also maintains the widely used open-source data analysis program BioXTAS RAW. Maintenance and upgrade plans cover improved liquid chromatography systems, data analysis software, and microfluidic mixers, as well as upgraded computing, data storage capabilities, network infrastructure, and detectors. We will fully optimize the instrument to take advantage of the world class beam that will be provided by the APS-U project. Last but not the least we will continue to increase automation for SAXS data acquisition in order to make the user as independent as possible.