Pathology Core

摘要-病理学核心 对老年动物的病理评估对于确定观察到的变化是否为 动物年龄与潜在的病理状况相关或无关。因此，它是必不可少的 以获得准确和透彻的老龄动物病理评估。病理核心将扮演一个 在圣安东尼奥内森休克衰老中心发挥关键作用，因为随着 年龄增加，并在很大程度上造成与年龄有关的发病率和死亡率。病理性知识 与用于研究衰老的干预措施相关的损害对于解释这些影响是必不可少的 对衰老过程的干预。这些知识还将提供对潜在的 干预效果背后的机制(S)。病理学核心将建立在丰富的经验基础上 圣安东尼奥大学的研究人员和核心领导者在实验病理分析方面的专业知识 动物模型。因此，病理学核心的具体目标是： 1.对已建立的和新的进行全面的临终和横断面病理分析 啮齿动物模型以及用于衰老研究的其他物种，这些物种自发死亡或在 由老龄化动物模型和寿命评估核心维护的群体中的特定年龄点。 2.进行免疫组织化学、分子和形态定量分析 啮齿动物模型和其他物种的组织/器官通过执行a)形态测量和 几种病变和年龄相关组织学改变的免疫组织化学分析及b)原位 杂交在分子水平上进一步分析组织学变化。 3.继续a)建立一个全面的组织病理学数据和图像数据库，作为资源 供调查人员分析，b)为新的调查提供基本病理信息，以及c) 通过收集和存储组织样本(石蜡和冰冻块)作为资源来创建组织档案 应要求对样品进行分析，并进行新的形态研究。 4.为调查人员提供组织病理学服务，准备石蜡和冰冻块，制作 未染色的玻片，并进行各种染色(包括特殊染色)。新增服务包括(A) 制作用于组织学/形态学实验的组织阵列载玻片，以及(B)执行激光捕获 用于进一步分子分析的显微解剖。 5.帮助对动物模型生物学基础感兴趣的教职员工和学生进行病理学研究 拨款申请和手稿准备所需的分析。

SUMMARY – PATHOLOGY CORE The pathological assessment of old animals is important to determine whether the changes observed as animals age are associated with or independent of underlying pathological conditions. It is, therefore, essential to obtain accurate and thorough pathological assessments of aging animals. The Pathology Core will play a key role in the San Antonio Nathan Shock Aging Center because pathology increases exponentially with advancing age and is largely responsible for age-related morbidity and mortality. Knowledge of the pathological lesions associated with interventions used to study aging is essential to interpreting the impact of these interventions on the aging process(es). This knowledge will also provide insight into the underlying mechanism(s) behind the interventions' effects. The Pathology Core will build on the extensive experience of researchers at San Antonio and the expertise of the Core Leaders in pathological analyses of experimental animal models. Thus, the Specific Aims of the Pathology Core are: 1. To conduct comprehensive end-of-life and cross-sectional pathological analyses of established and new rodent models as well as other species used in aging research that die spontaneously or are sacrificed at specific age points in colonies maintained by the Aging Animal Models and Longevity Assessment Core. 2. To conduct immunohistochemical, molecular, and quantitative morphometric analyses of the tissues/organs of rodent models and other species by performing a) morphometric and immunohistochemical analyses on several lesions and age-related histological changes and b) in situ hybridization to further analyze the histological changes at the molecular level. 3. To continue to a) accrue a comprehensive database of histopathological data and images as a resource for analyses by investigators, b) provide basic pathological information for new investigations, and c) create a tissue archive by collecting and storing tissue samples (paraffin and frozen blocks) as a resource for analyses of samples as requested and for new morphological research. 4. To provide histopathology services for investigators by preparing paraffin and frozen blocks, making unstained slides, and performing various staining (including special staining). New services include (a) making tissue array slides for histological/morphological experiments, and (b) performing laser capture microdissection for further molecular analyses. 5. To assist faculty and students interested in the basic biology of animal models with the pathological analyses needed for grant applications and manuscript preparation.