Neurogenetic Approaches to Study Directed Behavior in Drosophilla

项目总结： 长期目标是提供一个框架，以理解简单的、天生的行为是如何从 神经系统的活动。为此，分子遗传学技术、电生理学和成像 果蝇温度加工和偏爱机制的研究技术 该系统非常适合对神经回路和行为进行全面的遗传和分子解剖， 都是适用的。该实验室最近的研究表明，简单的感觉图代表了温度刺激 在苍蝇的大脑里。它还表明，二阶神经元的协调集合提取信息 关于温度变化的符号、开始、幅度和持续时间，从这个简单的地图。这项研究是 现在提议更深入地研究导致这种转变的细胞和分子机制 有可能。期望这项工作的结果将揭示新的机制和原则 神经系统中的躯体感觉处理，将补充关于区分功能的发现 在其他感觉方式中的提取，并将对更广泛的神经科学产生兴趣 社区，为神经回路内的信息处理工作提供信息。这项工作也有望 有助于对控制躯体感觉的神经回路的功能有全面的了解 动物的反应(温度和疼痛)，潜在地提供了对遗传和神经学的见解 影响神经元兴奋性的状况，会导致破坏性的医疗状况，如慢性疼痛。

PROJECT SUMMARY: The long term goal is to provide a framework to understand how a simple, innate behavior emerges from the activity of the nervous system. For this, molecular genetics techniques, electrophysiology and imaging technologies to study the mechanisms underlying temperature processing and preference in Drosophila -a system ideally suited for a comprehensive genetic and molecular dissection of neural circuits and behaviors, are applied. The lab's recent work has demonstrated that a simple sensory map represents temperature stimuli in the fly brain. It has also shown that a coordinated ensemble of second order neurons extracts information about the sign, onset, magnitude and duration of a temperature change from this simple map. The research is now proposing to delve deeper into the cellular and molecular mechanism that make this transformation possible. The expectation is that the results of this work will reveal new mechanisms and principles of somatosensory processing in the nervous system, will complement discoveries on differential feature extraction in other sensory modalities, and will have implications of interest to the broader neuroscience community, informing work on information processing within neural circuits. This work is also expected to contribute to the general understanding of the function of the neural circuits that control somatosensory responses (temperature and pain) in animals, potentially providing insights on genetic and neurological conditions which affect neuronal excitability resulting in devastating medical conditions such as chronic pain.