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Preservation of Nonhuman Primate Spermatozoa by Lyophilization

通过冻干保存非人灵长类动物精子

基本信息

批准号：
10610044
负责人：
Peter Gordon Barr-Gillespie
金额：
$ 26.45万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-05-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10610044
关键词：
Acrosome Address Animal Genetics Animal Model Animals Area Assisted Reproductive Technology Autologous Automation Autopsy Benchmarking Biological Assay Biomedical Research Breeding Callithrix Cattle Chromatin Structure Confocal Microscopy Core Grant Coupled Critical Pathways Cryopreservation DNA DNA Fragmentation Disease Disease model Drug or chemical Tissue Distribution Elements Embryo Embryonic Development Ensure Epididymis Equipment Equus caballus Event Failure Family suidae Formulation Foundations Freeze Drying Freezing Frozen Semen Future Genes Genetic Genetic Carriers Genetic Diseases Genome Genotype Germ Cells Goals Gold Hamsters Hydration status Insemination Intracytoplasmic Sperm Injections Investigation Laboratory Personnel Liquid substance Macaca Macaca fascicularis Macaca mulatta Macaca nemestrina Membrane Membrane Potentials Methods Modeling Modernization Modification Mus Nitrogen Oocytes Oregon Oryctolagus cuniculus Papio Phenotype Population Primates Procedures Process Production Protocols documentation Publishing Refrigeration Reporting Research Research Personnel Resource Sharing Resources Risk Sampling Shipping Specimen Sperm Motility Sperm Preservation Standardization Technology Temperature Testing Testis Texas Time Tissues United States National Institutes of Health Universities Vas deferens structure Washington Wisconsin base cohort cost embryo cell experience innovation mature animal mitochondrial membrane model development new technology nonhuman primate offspring oncofertility parent grant preservation prevent programs reproductive tract sperm cell sperm quality success tool

项目摘要

PROJECT SUMMARY The Oregon National Primate Research Center (ONPRC) is one of seven National Primate Research Centers (NPRCs) sponsored by the NIH. It is supported by the P51 Core Grant (P51-OD011092) and provides the expertise, technology, and resources required to deliver cutting-edge researchers access to nonhuman primates (NHPs) for biomedical investigation. The ONPRC maintains a colony of approximately 4900 breeding and research NHPs, the majority of which are rhesus macaques. Currently there is a national shortage of rhesus monkeys available for research, which is constraining attempts to preserve animals that are genetic carriers of rare or uncommon diseases. Successful preservation of gametes is a critical path forward to ensuring access to these animal models for future research programs. Additionally, there has been a tremendous shift in NHP model development from tissue-specific induced phenotypes in live animals to induced disease-specific genotypes in single-cell embryos later used to derive adult animals. As these gene- edited animals are difficult to achieve, preservation of gametes becomes a critical component to building disease model cohorts. With an increasing emphasis on gamete preservation as part of NHP model programs, alternatives to long-term gamete storage that does not require liquid nitrogen and costly storage or shipping strategies have become the new focus in NHP assisted reproductive technology (ART). In this supplement proposal to the parent grant, we will test the hypotheses that: 1) Modern freeze-drying (FD) equipment and simplex optimization will deduce optimal conditions for lyophilizing fresh collected rhesus macaque spermatozoa for long-term storage at common refrigeration temperatures; and 2) Spermatozoa collected post-mortem from testis, epididymis, and vas deferens can undergo lyophilization and long-term storage at common refrigeration temperatures for cynomolgus macaque, pigtailed macaque, baboon, and marmoset species. To test these hypotheses, simplex optimization of FD medium will be performed using previous published formulation attempts as a starting point. Elements such as medium components, pH, freezing format, and drying times will be assessed by standard sperm quality assays already performed in the ONPRC ART Core. Necropsy tissues from additional NHP species will be obtained from the Washington, Texas, and Wisconsin NPRCs through their tissue distribution programs and evaluated for differences in tissue origin lyophilization tolerance. Our findings will provide a benchmark for alternatives to NHP gamete storage and establish a foundation from which to continue efforts to refine and maximize opportunities for preserving valuable genetics.

项目总结