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Advanced Imaging Core

先进的成像核心

基本信息

批准号：
10608981
负责人：
John J Lemasters
金额：
$ 21.01万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10608981
关键词：
Address Animals Apoptosis Applications Grants Biological Sciences Biology Cell Membrane Permeability Cell Survival Cells Clinical Communities Computer Workstations Consultations Data Data Analyses Detection Development Digestive System Disorders Education Educational workshop Equipment Fluorescence Resonance Energy Transfer Fluorescent Probes Gases Gene Expression Generations Goals Hepatocyte Image Imaging Techniques Imaging technology Immersion Ions Journals Leadership Leukocytes Liver Liver diseases Medical Mentors Methods Microcirculation Microscope Microscopy Mitochondria Molecular Monitor Necrosis Optics Organism Pathology Permeability Phase Preparation Proteins Reporter Research Research Personnel Research Proposals Resource Sharing Resources Scanning Services Silicone Oils Slide South Carolina Specimen System Techniques Technology Temperature Three-Dimensional Imaging Tissue imaging Tissues Training Training Activity Universities Water Work cellular imaging digital imaging electrical potential fluorescence microscope fluorophore gastrointestinal high resolution imaging histological image histological slides image processing imaging approach imaging system immunocytochemistry innovation instrumentation intravital imaging intravital microscopy light microscopy liquid crystal polymer meetings member method development microscopic imaging molecular imaging multi-photon multiphoton microscopy multiplexed imaging new technology next generation novel optical imaging pathology imaging protein distribution pyridine nucleotide quasar second harmonic submicron success superresolution imaging superresolution microscopy ultra high resolution

项目摘要

Advanced Imaging Core - Abstract The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis, including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all microscopes are equipped with environmental chambers for temperature and gas phase control to allow non- destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super- resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and didactic training in current and next-generation imaging approaches are provided by seminars, demos and a biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core services promote the success of DDRCC investigator research on digestive and liver disease.

高级成像核心 - 摘要高级成像核心的总体目标是为 DDRCC 研究人员提供复杂的成功的高端细胞和组织成像和分析所需的技术、方法和培训，包括共焦、多光子和超分辨率显微镜、活体成像以及自动成像组织学幻灯片。具体目标是： 1) 为 DDRCC 研究人员提供最先进的光学能力显微镜检查； 2) 开发新颖的前沿成像应用程序，以满足新兴的科学需求 DDRCC 成员； 3) 提供有关复杂问题的专家指导、教育、培训和咨询光学成像技术使 DDRCC 研究人员能够进行科学发现，以及更广泛的研究 MUSC 研究社区。 Core 包含以下主要显微镜系统： 1) Zeiss LSM 880 NLO 配备 Quasar 光谱检测和快速 Airyscan 超分辨率的多光子/共焦系统能力; 2) Olympus Fluoview FV1200多光子显微镜，带有硅油光学器件，用于活体成像； 3） Olympus Fluoview FV 10i LIV 活细胞共聚焦显微镜，带水浸光学器件； 4) 蔡司 LSM 510 META 共焦显微镜； 5) BD BioSciences CARV II 盘扫描共焦显微镜，用于视频速率成像； 6） Zeiss Axiovert 200M宽场荧光显微镜； 7) Perkin-Elmer Vectra Polaris 自动化定量病理学成像系统。除专为组织学载玻片定制的 Vectra Polaris 外，所有显微镜配备了用于温度和气相控制的环境室，以允许非活细胞、组织和生物体的破坏性 3D 成像。主要应用包括1）活细胞超级参数指示荧光团的分辨率成像，用于监测离子、电势、自由基生成、吡啶核苷酸还原、膜通透性、细胞活力（凋亡和坏死）和亚微米荧光蛋白和其他荧光报告基因的分布； 2）组织切片的高分辨率成像用于免疫细胞化学和荧光蛋白分布； 3）荧光共振能量转移（FRET） DuoLink 用于表征和量化特定分子之间的相互作用； 4）活体显微镜检查监测微循环、白细胞边缘化、线粒体极化和通透性、自由基生成、活体动物的基因表达和其他参数； 5) 高通量、定量多重成像常规和免疫染色的临床和研究标本。计算机工作站提供离线图像处理/分析（Bitstream Imaris、ImageJ FIJI、Metamorph、IPLab 等）。动手实践和通过研讨会、演示和培训提供当前和下一代成像方法的教学培训每两年举办一次为期一周的查尔斯顿生物科学光学显微镜研讨会。先进的成像核心服务促进 DDRCC 研究者在消化和肝脏疾病方面的研究取得成功。