Advanced Imaging Core
先进的成像核心
基本信息
- 批准号:10608981
- 负责人:
- 金额:$ 21.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-15 至 2025-03-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimalsApoptosisApplications GrantsBiological SciencesBiologyCell Membrane PermeabilityCell SurvivalCellsClinicalCommunitiesComputer WorkstationsConsultationsDataData AnalysesDetectionDevelopmentDigestive System DisordersEducationEducational workshopEquipmentFluorescence Resonance Energy TransferFluorescent ProbesGasesGene ExpressionGenerationsGoalsHepatocyteImageImaging TechniquesImaging technologyImmersionIonsJournalsLeadershipLeukocytesLiverLiver diseasesMedicalMentorsMethodsMicrocirculationMicroscopeMicroscopyMitochondriaMolecularMonitorNecrosisOpticsOrganismPathologyPermeabilityPhasePreparationProteinsReporterResearchResearch PersonnelResearch ProposalsResource SharingResourcesScanningServicesSilicone OilsSlideSouth CarolinaSpecimenSystemTechniquesTechnologyTemperatureThree-Dimensional ImagingTissue imagingTissuesTrainingTraining ActivityUniversitiesWaterWorkcellular imagingdigital imagingelectrical potentialfluorescence microscopefluorophoregastrointestinalhigh resolution imaginghistological imagehistological slidesimage processingimaging approachimaging systemimmunocytochemistryinnovationinstrumentationintravital imagingintravital microscopylight microscopyliquid crystal polymermeetingsmembermethod developmentmicroscopic imagingmolecular imagingmulti-photonmultiphoton microscopymultiplexed imagingnew technologynext generationnoveloptical imagingpathology imagingprotein distributionpyridine nucleotidequasarsecond harmonicsubmicronsuccesssuperresolution imagingsuperresolution microscopyultra high resolution
项目摘要
Advanced Imaging Core - Abstract
The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated
technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis,
including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of
histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical
microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of
DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated
optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater
MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO
multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution
capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3)
Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META
confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6)
Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated
Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all
microscopes are equipped with environmental chambers for temperature and gas phase control to allow non-
destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super-
resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation,
pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron
distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections
for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET)
and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to
monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation,
gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging
of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline
image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and
didactic training in current and next-generation imaging approaches are provided by seminars, demos and a
biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core
services promote the success of DDRCC investigator research on digestive and liver disease.
高级成像核心 - 摘要
高级成像核心的总体目标是为 DDRCC 研究人员提供复杂的
成功的高端细胞和组织成像和分析所需的技术、方法和培训,
包括共焦、多光子和超分辨率显微镜、活体成像以及自动成像
组织学幻灯片。具体目标是: 1) 为 DDRCC 研究人员提供最先进的光学能力
显微镜检查; 2) 开发新颖的前沿成像应用程序,以满足新兴的科学需求
DDRCC 成员; 3) 提供有关复杂问题的专家指导、教育、培训和咨询
光学成像技术使 DDRCC 研究人员能够进行科学发现,以及更广泛的研究
MUSC 研究社区。 Core 包含以下主要显微镜系统: 1) Zeiss LSM 880 NLO
配备 Quasar 光谱检测和快速 Airyscan 超分辨率的多光子/共焦系统
能力; 2) Olympus Fluoview FV1200多光子显微镜,带有硅油光学器件,用于活体成像; 3)
Olympus Fluoview FV 10i LIV 活细胞共聚焦显微镜,带水浸光学器件; 4) 蔡司 LSM 510 META
共焦显微镜; 5) BD BioSciences CARV II 盘扫描共焦显微镜,用于视频速率成像; 6)
Zeiss Axiovert 200M宽场荧光显微镜; 7) Perkin-Elmer Vectra Polaris 自动化
定量病理学成像系统。除专为组织学载玻片定制的 Vectra Polaris 外,所有
显微镜配备了用于温度和气相控制的环境室,以允许非
活细胞、组织和生物体的破坏性 3D 成像。主要应用包括1)活细胞超级
参数指示荧光团的分辨率成像,用于监测离子、电势、自由基生成、
吡啶核苷酸还原、膜通透性、细胞活力(凋亡和坏死)和亚微米
荧光蛋白和其他荧光报告基因的分布; 2)组织切片的高分辨率成像
用于免疫细胞化学和荧光蛋白分布; 3)荧光共振能量转移(FRET)
DuoLink 用于表征和量化特定分子之间的相互作用; 4)活体显微镜检查
监测微循环、白细胞边缘化、线粒体极化和通透性、自由基生成、
活体动物的基因表达和其他参数; 5) 高通量、定量多重成像
常规和免疫染色的临床和研究标本。计算机工作站提供离线
图像处理/分析(Bitstream Imaris、ImageJ FIJI、Metamorph、IPLab 等)。动手实践和
通过研讨会、演示和培训提供当前和下一代成像方法的教学培训
每两年举办一次为期一周的查尔斯顿生物科学光学显微镜研讨会。先进的成像核心
服务促进 DDRCC 研究者在消化和肝脏疾病方面的研究取得成功。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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John J Lemasters其他文献
Ischemic preconditioning attenuates acute lung injury after partial liver transplantation
缺血预处理减轻部分肝移植后的急性肺损伤
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Qinlong Liu;Hasibur Rehman;Yasodha Krishnasamy;John J Lemasters;Zhi Zhong - 通讯作者:
Zhi Zhong
John J Lemasters的其他文献
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{{ truncateString('John J Lemasters', 18)}}的其他基金
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10210670 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10349589 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10597049 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mitochondrial depolarization, mitophagy, and mitochondrial DAMPs in ALD
ALD 中的线粒体去极化、线粒体自噬和线粒体 DAMP
- 批准号:
10155373 - 财政年份:2018
- 资助金额:
$ 21.01万 - 项目类别:
Mitochondrial depolarization, mitophagy, and mitochondrial DAMPs in ALD
ALD 中的线粒体去极化、线粒体自噬和线粒体 DAMP
- 批准号:
9920650 - 财政年份:2018
- 资助金额:
$ 21.01万 - 项目类别:
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