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Advanced Imaging Core

先进的成像核心

基本信息

批准号：
10395945
负责人：
John J Lemasters
金额：
$ 21.01万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10395945
关键词：
Address Animals Apoptosis Applications Grants Biological Sciences Biology Cell Membrane Permeability Cell Survival Cells Clinical Research Communities Computer Workstations Confocal Microscopy Consultations Custom Data Data Analyses Detection Development Digestive System Disorders Educational workshop Equipment Fluorescence Resonance Energy Transfer Fluorescent Probes Gases Gene Expression Generations Goals Hepatocyte Image Imaging Techniques Imaging technology Immersion Ions Journals Leadership Leukocytes Liver Liver diseases Medical Mentors Methods Microcirculation Microscope Microscopy Mitochondria Molecular Monitor Necrosis Optics Organism Pathology Permeability Phase Preparation Proteins Reporter Research Research Personnel Research Proposals Resolution Resource Sharing Resources Scanning Services Silicone Oils Slide South Carolina Specimen Stains System Techniques Technology Temperature Three-Dimensional Imaging Tissue imaging Tissues Training Training Activity Training and Education Universities Water Work base cellular imaging digital imaging electrical potential fluorescence microscope fluorophore gastrointestinal high resolution imaging histological image histological slides image processing imaging approach imaging system immunocytochemistry innovation instrumentation intravital imaging intravital microscopy light microscopy liquid crystal polymer meetings member method development microscopic imaging molecular imaging multiphoton microscopy multiplexed imaging new technology next generation novel optical imaging pathology imaging protein distribution pyridine nucleotide quasar second harmonic submicron success

项目摘要

Advanced Imaging Core - Abstract The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis, including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all microscopes are equipped with environmental chambers for temperature and gas phase control to allow non- destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super- resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and didactic training in current and next-generation imaging approaches are provided by seminars, demos and a biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core services promote the success of DDRCC investigator research on digestive and liver disease.

先进的成像核心-摘要