Advanced Imaging Core

先进的成像核心

• 批准号: 10395945
• 负责人: John J Lemasters
• 金额: $ 21.01万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10395945
• 关键词: Address; Animals; Apoptosis; Applications Grants; Biological Sciences; Biology; Cell Membrane Permeability; Cell Survival; Cells; Clinical Research; Communities; Computer Workstations; Confocal Microscopy; Consultations; Custom; Data; Data Analyses; Detection; Development; Digestive System Disorders; Educational workshop; Equipment; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Gases; Gene Expression; Generations; Goals; Hepatocyte; Image; Imaging Techniques; Imaging technology; Immersion; Ions; Journals; Leadership; Leukocytes; Liver; Liver diseases; Medical; Mentors; Methods; Microcirculation; Microscope; Microscopy; Mitochondria; Molecular; Monitor; Necrosis; Optics; Organism; Pathology; Permeability; Phase; Preparation; Proteins; Reporter; Research; Research Personnel; Research Proposals; Resolution; Resource Sharing; Resources; Scanning; Services; Silicone Oils; Slide; South Carolina; Specimen; Stains; System; Techniques; Technology; Temperature; Three-Dimensional Imaging; Tissue imaging; Tissues; Training; Training Activity; Training and Education; Universities; Water; Work; base; cellular imaging; digital imaging; electrical potential; fluorescence microscope; fluorophore; gastrointestinal; high resolution imaging; histological image; histological slides; image processing; imaging approach; imaging system; immunocytochemistry; innovation; instrumentation; intravital imaging; intravital microscopy; light microscopy; liquid crystal polymer; meetings; member; method development; microscopic imaging; molecular imaging; multiphoton microscopy; multiplexed imaging; new technology; next generation; novel; optical imaging; pathology imaging; protein distribution; pyridine nucleotide; quasar; second harmonic; submicron; success

Advanced Imaging Core - Abstract The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis, including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all microscopes are equipped with environmental chambers for temperature and gas phase control to allow non- destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super- resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and didactic training in current and next-generation imaging approaches are provided by seminars, demos and a biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core services promote the success of DDRCC investigator research on digestive and liver disease.

高级成像核心-摘要 高级成像核心的总体目标是为DDRCC研究人员提供复杂的 成功的高端细胞和组织成像和分析所需的技术、方法和培训， 包括共焦、多光子和超分辨率显微镜、活体成像和自动成像， 组织学切片具体目标是：1）为DDRCC研究人员提供最先进的光学功能， 2）开发新的，前线成像应用程序，以满足新兴的科学需求， （c）为复员方案协调委员会成员提供专家指导、教育、培训和咨询， 光学成像技术，使科学发现的DDRCC调查，以及更大的 MUSC研究社区。Core包含以下主要显微镜系统：1）Zeiss LSM 880 NLO 配备类星体光谱探测和快速Airyscan超分辨率的多光子/共焦系统 能力; 2）Olympus Fluoview FV 1200多光子显微镜，配有硅油光学器件，用于活体成像; 3） Olympus Fluoview FV 10 i LIV活细胞共聚焦显微镜，带水浸光学系统; 4）Zeiss LSM 510 Meta 共聚焦显微镜; 5）BD BioSciences CARV II盘式扫描共聚焦显微镜，用于视频速率成像; 6） Zeiss Axiovert 200 M宽视场荧光显微镜;和7）Perkin-Elmer Vectra Polaris自动化 定量病理成像系统。除了为组织学切片定制的Vectra Polaris外，所有 显微镜配备有用于温度和气相控制的环境室， 活细胞、组织和生物体的破坏性3D成像。主要应用包括1）活细胞超级- 参数指示荧光团的分辨率成像，以监测离子、电势、自由基生成， 吡啶核苷酸减少，膜通透性，细胞活力（凋亡和坏死）和亚微米 荧光蛋白和其他荧光报告物的分布; 2）组织切片的高分辨率成像 用于免疫细胞化学和荧光蛋白分布; 3）荧光共振能量转移（FRET） 和DuoLink来表征和量化特定分子之间的相互作用; 4）活体显微镜， 监测微循环、白细胞集落、线粒体极化和渗透性、自由基生成， 活动物中的基因表达和其它参数;和5）高通量、定量多路成像 常规和免疫染色的临床和研究标本。计算机工作站提供离线 图像处理/分析（Bitstream Imaris、ImageJ FIJI、Metamorph、IPLab等）。动手和 通过研讨会、演示和 两年一次为期一周的查尔斯顿生物科学光学显微镜讲习班。先进的成像核心 这些服务促进了DDRCC研究人员在消化和肝脏疾病方面的研究取得成功。