Advanced Imaging Core
先进的成像核心
基本信息
- 批准号:10395945
- 负责人:
- 金额:$ 21.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-15 至 2025-03-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimalsApoptosisApplications GrantsBiological SciencesBiologyCell Membrane PermeabilityCell SurvivalCellsClinical ResearchCommunitiesComputer WorkstationsConfocal MicroscopyConsultationsCustomDataData AnalysesDetectionDevelopmentDigestive System DisordersEducational workshopEquipmentFluorescence Resonance Energy TransferFluorescent ProbesGasesGene ExpressionGenerationsGoalsHepatocyteImageImaging TechniquesImaging technologyImmersionIonsJournalsLeadershipLeukocytesLiverLiver diseasesMedicalMentorsMethodsMicrocirculationMicroscopeMicroscopyMitochondriaMolecularMonitorNecrosisOpticsOrganismPathologyPermeabilityPhasePreparationProteinsReporterResearchResearch PersonnelResearch ProposalsResolutionResource SharingResourcesScanningServicesSilicone OilsSlideSouth CarolinaSpecimenStainsSystemTechniquesTechnologyTemperatureThree-Dimensional ImagingTissue imagingTissuesTrainingTraining ActivityTraining and EducationUniversitiesWaterWorkbasecellular imagingdigital imagingelectrical potentialfluorescence microscopefluorophoregastrointestinalhigh resolution imaginghistological imagehistological slidesimage processingimaging approachimaging systemimmunocytochemistryinnovationinstrumentationintravital imagingintravital microscopylight microscopyliquid crystal polymermeetingsmembermethod developmentmicroscopic imagingmolecular imagingmultiphoton microscopymultiplexed imagingnew technologynext generationnoveloptical imagingpathology imagingprotein distributionpyridine nucleotidequasarsecond harmonicsubmicronsuccess
项目摘要
Advanced Imaging Core - Abstract
The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated
technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis,
including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of
histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical
microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of
DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated
optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater
MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO
multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution
capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3)
Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META
confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6)
Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated
Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all
microscopes are equipped with environmental chambers for temperature and gas phase control to allow non-
destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super-
resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation,
pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron
distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections
for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET)
and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to
monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation,
gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging
of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline
image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and
didactic training in current and next-generation imaging approaches are provided by seminars, demos and a
biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core
services promote the success of DDRCC investigator research on digestive and liver disease.
先进的成像核心-摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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John J Lemasters其他文献
Ischemic preconditioning attenuates acute lung injury after partial liver transplantation
缺血预处理减轻部分肝移植后的急性肺损伤
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Qinlong Liu;Hasibur Rehman;Yasodha Krishnasamy;John J Lemasters;Zhi Zhong - 通讯作者:
Zhi Zhong
John J Lemasters的其他文献
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{{ truncateString('John J Lemasters', 18)}}的其他基金
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10210670 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10349589 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mechanisms of Mitochondrial Iron Uptake: New Therapeutic Targets in Hepatotoxicity
线粒体铁摄取机制:肝毒性的新治疗靶点
- 批准号:
10597049 - 财政年份:2021
- 资助金额:
$ 21.01万 - 项目类别:
Mitochondrial depolarization, mitophagy, and mitochondrial DAMPs in ALD
ALD 中的线粒体去极化、线粒体自噬和线粒体 DAMP
- 批准号:
10155373 - 财政年份:2018
- 资助金额:
$ 21.01万 - 项目类别:
Mitochondrial depolarization, mitophagy, and mitochondrial DAMPs in ALD
ALD 中的线粒体去极化、线粒体自噬和线粒体 DAMP
- 批准号:
9920650 - 财政年份:2018
- 资助金额:
$ 21.01万 - 项目类别:
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