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Advanced Imaging Core

先进的成像核心

基本信息

批准号：
10586110
负责人：
John J Lemasters
金额：
$ 14.05万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-03 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586110
关键词：
Animals Apoptosis Award Biological Sciences Cell Membrane Permeability Cell Survival Cells Centers of Research Excellence Clinical Communities Computer Workstations Computer software Consultations Data Data Analyses Detection Digestive System Disorders Education Ensure Equipment Fluorescence Resonance Energy Transfer Gases Gene Expression Generations Goals Grant Image Imaging technology Immersion Individual Ions Laboratories Lasers Leukocytes Liver diseases Medicine Mentors Microcirculation Microscope Microscopy Mission Mitochondria Monitor Necrosis Optics Organism Pathology Permeability Phase Preparation Proteins Reporter Research Research Personnel Scanning Services Silicone Oils Slide Specimen System Technology Temperature Three-Dimensional Imaging Time Tissue imaging Tissues Training Water Work cell fixing digital imaging electrical potential fluorescence microscope fluorophore high resolution imaging histological image histological slides image processing imaging approach imaging modality imaging system immunocytochemistry intravital imaging intravital microscopy liquid crystal polymer live cell imaging microscopic imaging multi-photon multiphoton microscopy multiplexed imaging next generation pathology imaging protein distribution pyridine nucleotide quasar submicron success superresolution microscopy ultra high resolution

项目摘要

Advanced Imaging Core – Project Summary The COBRE in Digestive & Liver Disease (CDLD) Advanced Imaging Core provides equipment, expertise and training to CDLD investigators for state-of-the-art cell- and tissue-based imaging, including confocal, multiphoton and super-resolution microscopy of live and fixed cells and tissues, intravital imaging and automated imaging of histological slides. The Advanced Imaging Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO multiphoton/confocal system equipped with a Coherent Chameleon multi-photon laser, Quasar spectral detection and Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with SpectraPhysics MaiTai DeepSee laser and silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all microscopes are equipped with environmental chambers for temperature and gas phase control to allow non-destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell imaging of parameter- sensitive fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis), and the submicron distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immunostained clinical and research specimens. Ancillary equipment required for specimen preparation is also provided. Consultation and services for TEM and SEM are available through the Department of Pathology & Laboratory Medicine. Computer workstations provide offline image processing/analysis (ImageJ FIJI, Metamorph, IPLab and other software). Advanced Imaging Core services provide training and assistance to promote the success of individual CDLD investigators.

高级成像核心 – 项目摘要 COBRE 消化和肝脏疾病 (CDLD) 高级成像核心提供设备、专业知识和为 CDLD 研究人员提供最先进的细胞和组织成像培训，包括共焦、多光子活体和固定细胞和组织的超分辨率显微镜、活体成像和自动成像组织学幻灯片。高级成像核心包含以下主要显微镜系统：1) Zeiss LSM 880 NLO 多光子/共焦系统，配备 Coherent Chameleon 多光子激光器、Quasar 光谱检测和Airyscan超分辨率能力； 2) 奥林巴斯 Fluoview FV1200 多光子显微镜用于活体成像的 SpectraPhysics MaiTai DeepSee 激光和硅油光学器件； 3）奥林巴斯Fluoview FV 10i 带水浸光学器件的 LIV 活细胞共焦显微镜； 4）蔡司LSM 510 META共焦显微镜； 5） BD BioSciences CARV II 盘扫描共焦显微镜，用于视频速率成像； 6）蔡司Axiovert 200M 宽视野荧光显微镜； 7) Perkin-Elmer Vectra Polaris 自动定量病理学成像系统。除了专为组织学载玻片定制的 Vectra Polaris 外，所有显微镜均采用配备用于温度和气相控制的环境室，以实现无损 3D 活细胞、组织和生物体的成像。主要应用包括 1) 参数的活细胞成像敏感荧光团可监测离子、电位、自由基生成、吡啶核苷酸还原、膜通透性、细胞活力（细胞凋亡和坏死）以及荧光的亚微米分布蛋白质和其他荧光报告基因； 2) 免疫细胞化学组织切片高分辨率成像和荧光蛋白分布； 3）荧光共振能量转移（FRET）和DuoLink 表征和量化特定分子之间的相互作用； 4）活体显微镜监测微循环、白细胞边缘化、线粒体极化和通透性、自由基生成、基因活体动物的表达和其他参数； 5) 高通量、定量多重成像常规和免疫染色的临床和研究标本。标本所需辅助设备还提供了准备工作。该部门可提供 TEM 和 SEM 的咨询和服务病理学和检验医学。计算机工作站提供离线图像处理/分析（ImageJ FIJI、Metamorph、IPLab 等软件）。高级成像核心服务提供培训和帮助促进个人 CDLD 调查人员的成功。