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Advanced Imaging Core

先进的成像核心

基本信息

批准号：
10337321
负责人：
John J Lemasters
金额：
$ 21.01万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-03 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10337321
关键词：
Animals Apoptosis Award Back Biological Sciences Cell Membrane Permeability Cell Survival Cells Centers of Research Excellence Charge Clinical Research Communities Computer Workstations Computer software Confocal Microscopy Consultations Custom Data Data Analyses Detection Digestive System Disorders Ensure Equipment Fluorescence Resonance Energy Transfer Gases Gene Expression Generations Goals Grant Image Imaging technology Immersion Individual Ions Laboratories Lasers Leukocytes Liver diseases Medicine Mentors Microcirculation Microscope Microscopy Mission Mitochondria Monitor Necrosis Optics Organism Pathology Permeability Phase Preparation Proteins Reporter Research Research Personnel Resolution Scanning Services Silicone Oils Slide Specimen System Technology Temperature Three-Dimensional Imaging Time Tissue imaging Tissues Training Training and Education Water Work advanced disease base digital imaging electrical potential fluorescence microscope fluorophore high resolution imaging histological image histological slides image processing imaging approach imaging modality imaging system immunocytochemistry intravital imaging intravital microscopy liquid crystal polymer live cell imaging microscopic imaging multi-photon multiphoton microscopy multiplexed imaging next generation pathology imaging protein distribution pyridine nucleotide quasar submicron success

项目摘要

Advanced Imaging Core – Project Summary The COBRE in Digestive & Liver Disease (CDLD) Advanced Imaging Core provides equipment, expertise and training to CDLD investigators for state-of-the-art cell- and tissue-based imaging, including confocal, multiphoton and super-resolution microscopy of live and fixed cells and tissues, intravital imaging and automated imaging of histological slides. The Advanced Imaging Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO multiphoton/confocal system equipped with a Coherent Chameleon multi-photon laser, Quasar spectral detection and Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with SpectraPhysics MaiTai DeepSee laser and silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all microscopes are equipped with environmental chambers for temperature and gas phase control to allow non-destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell imaging of parameter- sensitive fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis), and the submicron distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immunostained clinical and research specimens. Ancillary equipment required for specimen preparation is also provided. Consultation and services for TEM and SEM are available through the Department of Pathology & Laboratory Medicine. Computer workstations provide offline image processing/analysis (ImageJ FIJI, Metamorph, IPLab and other software). Advanced Imaging Core services provide training and assistance to promote the success of individual CDLD investigators.

高级成像核心-项目摘要