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Analytical Cell Models Core

分析单元模型核心

基本信息

批准号：
10608972
负责人：
STEPHEN A DUNCAN
金额：
$ 25.07万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10608972
关键词：
Adopted Affect Animal Model CRISPR/Cas technology Cell Culture Techniques Cell Differentiation process Cell Line Cell Maintenance Cell Separation Cell model Cells Cytometry DNA Sequence Alteration Digestive System Disorders Ensure Evaluation Flow Cytometry Gastrointestinal tract structure Generations Genetic Variation Genome engineering Goals Hepatocyte Human Infrastructure Laboratories Liver Liver diseases Medical Modeling Mutate Mutation Organoids Pathogenesis Patients Pluripotent Stem Cells Procedures Production Protocols documentation Reagent Research Research Personnel Rodent Running South Carolina Technology Testing Tissues Training Universities Update Wisconsin analytical tool cholangiocyte differentiation protocol established cell line experience genome editing human model induced pluripotent stem cell injured medical schools member new technology professor programs stem cell biology tissue culture tool

项目摘要

Analytical Cell Models Core - Abstract The DDRCC Analytical Cell Models Core proposes to provide DDRCC members with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by DDRCC members. The Core will also provide members with primary parenchymal and non- parenchymal livers cells from both human and rodent livers as well as critical tools for the analyses of cells. In addition to providing DDRCC members with cell lines that can be used for direct analyses, the Core will also provide members with training and critical reagents that will allow them to establish many of the procedures in their own laboratories. The Core will adopt a program of rigorous evaluation that will ensure that it performs optimally and continues to provide DDRCC members access to new technologies as they are developed. Dr. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. He will be supported by Dr. Don Rockey who has extensive experience in the isolation of cells from both human and rodent livers.

分析细胞模型核心-摘要 DDRCC分析单元模型核心建议为DDRCC成员提供最先进的消化和肝脏疾病的组织培养模型。我们将使用基因工程技术， CRIPSR-Cas9将遗传改变引入已建立的细胞系和诱导多能干细胞（iPSC）。iPSC将分化形成胃肠道和肝脏的细胞，这些细胞将用于 DDRCC成员的研究。核心还将为成员提供初级实质和非实质的来自人类和啮齿动物肝脏的肝实质细胞以及细胞分析的关键工具。在除了向DDRCC成员提供可用于直接分析的细胞系外，核心小组还将为成员提供培训和关键试剂，使他们能够建立许多程序，自己的实验室。核心将采用严格的评估程序，以确保其执行最佳地，并继续向DDRCC成员提供获得新技术的机会。博士邓肯，谁将领导核心，是一个专家在干细胞生物学和细胞分化，他已经使用基因组广泛地工程化以产生细胞来模拟肝脏疾病。他以前有过类似的经验。他是威斯康星州医学院的教授。他将得到唐·罗基博士的支持，在从人类和啮齿动物肝脏分离细胞方面拥有丰富的经验。