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Cell Models Core

细胞模型核心

基本信息

批准号：
10586108
负责人：
STEPHEN A DUNCAN
金额：
$ 16.24万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-03 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586108
关键词：
Adopted Affect Animal Disease Models Biological Models CRISPR/Cas technology Cell Culture Techniques Cell Differentiation process Cell Line Cell Maintenance Cell model Cells Centers of Research Excellence Collaborations Colon Communities DNA Sequence Alteration Data Development Digestive System Disorders Drug Screening Ensure Enteroendocrine Cell Epithelial Cells Evaluation Feedback Funding Future Gastrointestinal tract structure Generations Genes Genome engineering Goals Goblet Cells Hepatocyte Human Implant Investigation Laboratories Leadership Liver Liver diseases Medical Mentors Mesenchyme Modeling Molecular Mutate Mutation Needs Assessment Organoids Paneth Cells Performance Pluripotent Stem Cells Printing Procedures Production Program Evaluation Protocols documentation Reagent Reproducibility Research Research Personnel Resources Running Self-Examination Services South Carolina Surveys System Technical Expertise Technology Tissues Training Universities Wisconsin Work cell type cholangiocyte directed differentiation established cell line experience experimental study genetically modified cells human pluripotent stem cell in vivo induced pluripotent stem cell innovation intestinal epithelium medical schools mouse model new technology operation professor programs recruit stable cell line stem cell biology stem cells tissue culture tool training opportunity

项目摘要

Cell Models Core – Project Summary The COBRE in Digestive & Liver Disease (CDLD) Cell Models Core will provide CDLD investigators with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce specific genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by CDLD investigators. In addition to providing CDLD investigators cell lines and organoids that can be used for direct analyses, the Cell Models Core will also equip investigators with mentoring, training, and critical reagents allowing them to establish the procedures in their own laboratories. Through training opportunities, the Core will advance the technological toolbox of CDLD investigators, increase their competitiveness for funding, and enable them to advance as experts in digestive and liver disease research. The Core will also adopt a program of rigorous evaluation to ensuring optimal performance and continual access to new technologies as they are developed. The Cell Models Core represents a new core research resource at the Medical University of South Carolina. Currently, this technology is only available to a limited group of investigators and is temporarily run from the Duncan laboratory. Dr. Stephen A. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. The Core also recruited the expertise of Dr. Jorge Múnera who has pioneered the generation of colonic organoids from iPSCs. The Core leadership has already worked closely with the CDLD Junior Investigators to generate preliminary data for their proposed projects. Support through the COBRE will allow the Cell Models Core to expand its operation and provide these essential services to a broad community of CDLD investigators. A needs assessment survey ascertained that 21 established CDLD investigators and 3 junior investigators would use the core in the immediate future. The core will therefore have a substantial impact and advance digestive disease and liver research at MUSC.

细胞模型核心 – 项目摘要消化和肝脏疾病 (CDLD) 细胞模型核心中的 COBRE 将为 CDLD 研究人员提供访问权限最先进的消化和肝脏疾病组织培养模型。我们将使用基因组工程包括 CRIPSR-Cas9 在内的技术可将特定的遗传改变引入已建立的细胞系和诱导多能干细胞（iPSC）。 iPSC 将分化形成胃肠道细胞和肝脏，将用于 CDLD 研究人员的研究。除了为 CDLD 研究人员提供细胞系和可用于直接分析的类器官，细胞模型核心还将为研究人员提供指导、培训和关键试剂使他们能够在自己的实验室中建立程序。通过培训机会，核心将推进 CDLD 调查人员的技术工具箱，提高他们在资金方面的竞争力，并使他们能够成为消化和肝脏疾病研究领域的专家。核心还将采用严格的评估计划，以确保最佳性能和持续访问新技术的开发。细胞模型核心代表了新的核心研究资源南卡罗来纳医科大学。目前，该技术仅适用于有限的群体调查员并暂时由邓肯实验室运行。 Stephen A. Duncan 博士将领导核心团队，是干细胞生物学和细胞分化方面的专家，他广泛使用基因组工程来生成细胞来模拟肝病。他之前在担任教授期间曾有过运行类似核心的经验。威斯康星医学院。 Core 还聘请了 Jorge Múnera 博士的专业知识，他开创了从 iPSC 生成结肠类器官。核心领导层已与 CDLD 密切合作初级研究人员为他们提议的项目生成初步数据。通过 COBRE 提供支持允许 Cell Models Core 扩大其运营并向广泛的社区提供这些基本服务 CDLD 调查员。一项需求评估调查确定，21 名 CDLD 调查员和 3 名初级研究人员将在不久的将来使用该核心。因此，核心将产生重大影响并推进 MUSC 的消化疾病和肝脏研究。