Cell Models Core

细胞模型核心

• 批准号: 10337320
• 负责人: STEPHEN A DUNCAN
• 金额: $ 24.28万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-03 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10337320
• 关键词: Adopted; Affect; Animal Disease Models; Biological Models; CRISPR/Cas technology; Cell Culture Techniques; Cell Differentiation process; Cell Line; Cell Maintenance; Cell model; Cells; Centers of Research Excellence; Communities; DNA Sequence Alteration; Data; Development; Digestive System Disorders; Drug Screening; Ensure; Enteroendocrine Cell; Epithelial Cells; Evaluation; Feedback; Funding; Future; Gastrointestinal tract structure; Generations; Genes; Genome engineering; Goals; Goblet Cells; Hepatocyte; Human; Implant; Investigation; Laboratories; Lead; Leadership; Liver; Liver diseases; Medical; Mentors; Mesenchyme; Modeling; Molecular; Mutate; Mutation; Needs Assessment; Organoids; Paneth Cells; Performance; Printing; Procedures; Production; Program Evaluation; Protocols documentation; Reagent; Reproducibility; Research; Research Personnel; Resources; Running; Self-Examination; Services; South Carolina; Surveys; System; Technical Expertise; Technology; Time; Tissues; Training; Universities; Wisconsin; Work; base; cell type; cholangiocyte; directed differentiation; established cell line; experience; experimental study; genetically modified cells; human pluripotent stem cell; in vivo; induced pluripotent stem cell; innovation; intestinal epithelium; medical schools; mouse model; new technology; operation; professor; programs; recruit; stable cell line; stem cell biology; stem cells; tissue culture; tool; training opportunity

Cell Models Core – Project Summary The COBRE in Digestive & Liver Disease (CDLD) Cell Models Core will provide CDLD investigators with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce specific genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by CDLD investigators. In addition to providing CDLD investigators cell lines and organoids that can be used for direct analyses, the Cell Models Core will also equip investigators with mentoring, training, and critical reagents allowing them to establish the procedures in their own laboratories. Through training opportunities, the Core will advance the technological toolbox of CDLD investigators, increase their competitiveness for funding, and enable them to advance as experts in digestive and liver disease research. The Core will also adopt a program of rigorous evaluation to ensuring optimal performance and continual access to new technologies as they are developed. The Cell Models Core represents a new core research resource at the Medical University of South Carolina. Currently, this technology is only available to a limited group of investigators and is temporarily run from the Duncan laboratory. Dr. Stephen A. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. The Core also recruited the expertise of Dr. Jorge Múnera who has pioneered the generation of colonic organoids from iPSCs. The Core leadership has already worked closely with the CDLD Junior Investigators to generate preliminary data for their proposed projects. Support through the COBRE will allow the Cell Models Core to expand its operation and provide these essential services to a broad community of CDLD investigators. A needs assessment survey ascertained that 21 established CDLD investigators and 3 junior investigators would use the core in the immediate future. The core will therefore have a substantial impact and advance digestive disease and liver research at MUSC.

单元模型核心-项目摘要 消化和肝病(CDLD)细胞模型核心将为CDLD研究人员提供 最先进的消化系统和肝脏疾病的组织培养模型。我们将使用基因组工程 包括CRIPSR-Cas9在内的技术将特定的基因改变引入已建立的细胞系和 诱导多能干细胞(IPSCs)。IPSCs将被分化成胃肠道细胞 以及将用于CDLD调查人员研究的肝脏。除了为CDLD研究人员提供细胞系 和可用于直接分析的有机化合物，细胞模型核心还将为调查人员配备 指导、培训和关键试剂，使他们能够在自己的实验室建立程序。 通过培训机会，核心将推进CDLD调查人员的技术工具箱，增加 提高他们在资金方面的竞争力，并使他们能够作为消化系统和肝病研究方面的专家获得提升。 核心还将采用一项严格的评估计划，以确保最佳性能和持续访问 随着新技术的发展。Cell Models Core是一种新的核心研究资源，位于 南卡罗来纳医科大学。目前，这项技术只适用于有限的一群人 调查人员，暂时从邓肯实验室运行。将领导核心的史蒂芬·A·邓肯博士， 是干细胞生物学和细胞分化方面的专家，他广泛使用基因组工程来 产生细胞来模拟肝病。他以前在管理类似的核心方面有过经验，当时他是 威斯康星医学院。核心还招募了豪尔赫·穆内拉博士的专业知识，他开创了 从ipscs产生结肠类器官。核心领导层已经与民盟密切合作 初级调查人员为他们提议的项目生成初步数据。通过Cobre提供的支持将 允许Cell Models Core扩展其业务，并向更广泛的社区提供这些基本服务 CDLD调查员的名单。一项需求评估调查确定，21名CDLD调查员和3名 初级调查人员将在不久的将来使用核心。因此，核心将产生重大影响 并在MUSC推进消化系统疾病和肝脏研究。