Mechanisms of Activity-dependent Microglia-neuron Interactions in Development and Disease

发育和疾病中活动依赖性小胶质细胞-神经元相互作用的机制

• 批准号: 10611898
• 负责人: Beth Ann Stevens
• 金额: $ 202.21万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611898
• 关键词: Mechanisms; Activity; dependent; Microglia; neuron

Summary Microglia-mediated synaptic pruning is highly regulated during developmental critical periods of synaptic refinement, but its activation in vulnerable brain regions in disease models suggests that disease and development share common regulators and mechanisms of pruning. Synaptic refinement is an activity- dependent process where weak synapses are preferentially pruned. We hypothesize that neural activity is a key upstream activator and regulator of microglia-mediated pruning in development and disease. In support of this hypothesis, we and others demonstrated that microglia phagocytose synaptic elements and are capable of sensing and responding to activity-related signals as they preferentially engulf less active synapses. However, how microglia determine which synapses to engulf and which to avoid, and the identity of the upstream neuronal signals that detect neural activity and transmit this information to microglia are not known. In the immune system, phagocytosis is carefully governed by both phagocytic, “eat me” and anti- phagocytic, “don’t eat me” molecules. In the brain, we identified neuronal CD47, a “don’t eat me” signal protects synapses from inappropriate removal. We further found that exposed phosphatidylserine (PS), an “eat me signal” drives microglial recognition of synapses for engulfment. Furthermore, we have identified the tyrosine kinases, Pyk2 and JAK2 as neuronal signals that are activated at inactive synapses and necessary for the elimination of these inputs. Finally, we found that PS exposure is elevated early in the hippocampus in an Alzheimer's disease (AD) mouse model. Based on these and other data, we propose to test the hypothesis that activity-dependent signals within neurons, such as Pyk2 and JAK2, dynamically regulate "eat me" and "don't eat me" signals on synapses to drive proper microglial phagocytosis of inactive synapses. We further hypothesize that aberrant activation of such signals lead to abnormal synapse loss in neurodegenerative diseases, such as Alzheimer’s Disease (AD). Specifically we aim to test the following: Aim 1) Investigate mechanisms of activity-regulated PS exposure and function at developing synapses; Aim 2: Determine activity-dependent neuronal signaling that regulates "eat me" and "don't eat me" signals for microglial engulfment; and Aim 3: Investigate the mechanisms of early synaptic dysfunction and microglial synaptic targeting associated with AD. We will use interdisciplinary in vivo and in vitro approaches with molecular/cell biological, histological, mouse genetic, imaging, and electrophysiological techniques with various newly developed systems to address our aims. Our studies could also provide new targets for therapeutic intervention, as restoring the balance of protective and elimination signals could protect against synapse loss in early stages of AD and other diseases.

总结

项目成果

Microglial depletion disrupts normal functional development of adult-born neurons in the olfactory bulb.

小胶质细胞的耗竭会破坏嗅球中成年神经元的正常功能发育。

• DOI: 10.7554/elife.50531
• 发表时间: 2020
• 期刊: eLife
• 影响因子: 7.7
• 作者: Wallace,Jenelle;Lord,Julia;Dissing-Olesen,Lasse;Stevens,Beth;Murthy,VenkateshN
• 通讯作者: Murthy,VenkateshN