Heterogeneity in bacterial quorum sensing during infection

感染过程中细菌群体​​感应的异质性

• 批准号: 10625753
• 负责人: Timothy Miyashiro
• 金额: $ 7.06万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625753
• 关键词: Affect; Animals; Attenuated; Bacteria; Biological Models; Bioluminescence; Development; Enzymes; Euprymna scolopes; Generations; Goals; Growth; Hawaiian; Heterogeneity; Infection; Light; Link; Luciferases; Microbe; Microbiology; Nutrient; Organ; Oxidative Stress; Physiological; Population; Research; Resistance; Squid; Sulfur; Symbiosis; System; Training; Vibrio fischeri; Work; base; career; experimental study; fitness; host colonization; host-microbe interactions; mutant; quorum sensing; symbiont; trait

PROJECT SUMMARY Animals harbor bacterial symbionts that express specific traits that promote host fitness. How hosts select for bacteria with those traits remains poorly understood. A particularly useful model system to examine host- microbe symbioses is the symbiosis established between the Hawaiian bobtail squid Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri. The host harbors population of V. fischeri within a specialized light organ. In exchange for bioluminescence, the host provides the bacterial symbionts with nutrients that promote their growth. The overall goal of this proposal is to increase understanding of how squid select for bioluminescent symbionts. Previous work has shown that mutants that lack the light-producing enzyme luciferase are able to colonize the light organ but become attenuated. Research efforts with mutants that lack key factors that enable resistance to oxidative stress will be examined in colonization experiments. A screen to identify systems of nutrient import that is linked to the generation of oxidative stress will also be performed. Together, these results will determine the physiological changes that affect nonluminous strains, which will increase understanding of how hosts select for functional symbionts. The candidate will receive training in microbiology research and opportunities for professional development, which will prepare the candidate for a career in microbiology research.

项目摘要 动物体内有细菌共生体，它们表现出促进宿主适应性的特定特征。主机如何选择 对具有这些特征的细菌的了解仍然很少。一个特别有用的模型系统来检查主机- 微生物共生是夏威夷短尾乌贼Euprymna spectroopes和 生物发光细菌费氏弧菌宿主在一个专门的 轻风琴作为生物发光的交换，宿主为细菌共生体提供营养， 促进他们的成长。这项建议的总体目标是增加对鱿鱼如何选择的理解， 发光共生体先前的研究表明，缺乏发光酶的突变体 荧光素酶能够定殖于发光器官，但变得减弱。对突变体的研究 将在定殖实验中检查能够抵抗氧化应激的关键因素。的屏幕 还将确定与氧化应激产生相关的营养输入系统。 总之，这些结果将确定影响非发光菌株的生理变化， 增加对主机如何选择功能性共生体的理解。候选人将接受以下培训： 微生物学研究和专业发展的机会，这将为候选人准备一个 从事微生物学研究。