Heterogeneity in bacterial quorum sensing during infection

感染过程中细菌群体​​感应的异质性

• 批准号: 10261900
• 负责人: Timothy Miyashiro
• 金额: $ 3.39万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10261900
• 关键词: ATP Hydrolysis; Affect; Bacteria; Bacterial Genome; Binding Proteins; Cell physiology; Cells; Development; Euprymna scolopes; Family; Gene Expression; Genetic Transcription; Goals; Hawaiian population; Heterogeneity; Human; Infection; Invertebrates; Light; Microbiology; Organ; Organism; Proliferating; Research; Sigma Factor; Training; Transcription Initiation; Vibrio fischeri; host-microbe interactions; mutant; promoter; quorum sensing; spatiotemporal; transcription factor; transcriptomics

PROJECT SUMMARY Bacteria must express certain factors to successfully colonize and proliferate within a host. Many factors are controlled at the level of transcription. Roughly 60% of bacterial genomes encode the alternative sigma factor sigma-54, which activate a subset of promoters. Transcriptional initiation of sigma-54-dependent promoter requires energy from the hydrolysis of ATP, which is catalyzed by a bacterial enhance binding protein that binds upstream of the promoter. The overall goal of this proposal is to increase understanding of how bEBPs promote initial host-microbe interactions. To achieve this goal, the bEBPs encoded within the bacterium Vibrio fischeri will be investigated for their impact on the ability to associated with its natural host. V. fischeri cells colonize and grow within the light organs of certain invertebrates including the Hawaiian bobtail squid Euprymna scolopes. Research efforts with defined bEBP mutants include colonization analyses, which will determine the extent to which each bEBP impacts the ability of V. fischeri to colonize its host. Transcriptomic analyses are also proposed to determine how each bEBP affects cellular physiology of V. fischeri. Together, these results will determine the contribution of each bEBP to the ability of V. fischeri to establish a long-term association with its host. The candidate will receive post-baccalaureate training in microbiology research and opportunities for professional development, which will prepare the candidate for graduate studies in microbiology.

项目摘要 细菌必须表达某些因子才能在宿主体内成功定植和增殖。许多因素 是在转录水平上控制的。大约60%的细菌基因组编码交替的sigma 因子σ-54，其激活启动子的子集。sigma-54依赖的转录起始 启动子需要来自ATP水解的能量，这是由细菌增强结合蛋白催化的 结合在启动子的上游本提案的总体目标是增加对如何 bEBPs促进初始宿主-微生物相互作用。为了实现这一目标，编码在 将研究费氏弧菌对其与天然宿主结合能力的影响。诉 费氏细胞在某些无脊椎动物（包括夏威夷短尾鱼）的发光器官中定居和生长 鱿鱼对确定的bEBP突变体的研究工作包括定殖分析， 将决定每个bEBP影响费氏弧菌定殖其宿主的能力的程度。 转录组学分析也被提出来确定每个bEBP如何影响V的细胞生理学。 fischeri。总之，这些结果将确定每个bEBP对费氏弧菌的能力的贡献， 与宿主建立长期的联系。候选人将接受学士后培训， 微生物学研究和专业发展的机会，这将准备候选人 微生物学研究生课程。