Flagellin regulation by Vibrio fischeri during colonization and presistence

费氏弧菌在定植和生存过程中鞭毛蛋白的调节

• 批准号: 7642300
• 负责人: Timothy Miyashiro
• 金额: $ 4.72万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7642300
• 关键词: Address; Bacteria; Binding Sites; Cells; Color; Environment; Epithelial; Flagellin; Fluorescence; Fluorescence Microscopy; Gene Expression; Genes; Genetic; Hawaiian population; Health; In Situ; Individual; Light; Link; Measures; Microbe; Molecular; Mutagenesis; Organ; Organism; Positioning Attribute; Regulation; Reporter; Research; Role; Signaling Molecule; Squid; Surface; Symbiosis; System; Techniques; Testing; Time; Tissues; Vibrio fischeri; Virulence; base; cell motility; gene function; in vivo; insight; interest; pathogenic bacteria; research study; tool

DESCRIPTION (provided by applicant): The objective of this research is to investigate the molecular mechanisms bacteria use to specifically interact with eukaryotic hosts. By taking advantage of the natural symbiosis between the Hawaiian squid Euprymna scolopes and its bacterial symbiont Vibrio fischeri, the proposed experiments will explore the role of bacterial motility during host colonization by a beneficial microbe. This natural host-bacteria system is amenable to modern genetic tools and in vivo analysis with confocal fluorescence microscopy. The specific aims of this research application are: 1) to characterize each type of flagellin during initial colonization of the squid light organ; and, 2) to identify the bacterial determinants, including those involved in motility, that govern the long-term persistence of V. fischeri in E. scolopes. In addition to elucidating the role of bacterial motility in colonization and persistence, the results of the proposed experiments will shed light on the link between motility and the expression of virulence genes found in many pathogenic bacteria. Furthermore, insight into the interactions between eukaryotic hosts and their natural symbiotic microbes will help us understand the impact that microbes have on our own health.

描述（由申请人提供）：本研究的目的是研究细菌用于与真核宿主特异性相互作用的分子机制。通过利用夏威夷鱿鱼 Euprymna scolopes 与其细菌共生体费氏弧菌之间的自然共生关系，拟议的实验将探索细菌运动在有益微生物定植宿主过程中的作用。这种天然宿主细菌系统适合现代遗传工具和共焦荧光显微镜的体内分析。本研究应用的具体目的是：1）表征鱿鱼光器官初始定植过程中每种类型的鞭毛蛋白； 2) 确定细菌决定因素，包括那些参与运动的决定因素，这些决定因素控制着费氏弧菌在蜈蚣中的长期存留。除了阐明细菌运动在定植和持久性中的作用之外，所提出的实验结果还将揭示运动性与许多病原细菌中发现的毒力基因表达之间的联系。此外，深入了解真核宿主与其天然共生微生物之间的相互作用将有助于我们了解微生物对我们自身健康的影响。