Heterogeneity in bacterial quorum sensing during infection

项目总结 所有动物都依赖细菌共生体来获得特定的特征，以促进它们的健康。这些机制由 目前尚不清楚哪些东道主获得并选择了这些共生体，但对开发新战略很重要 以及以细菌为基础的治疗方法，以改善宿主健康。夏威夷人之间建立的共生关系 短尾鱿鱼和生物发光细菌费氏弧菌是检测分子的有力模型。 促进共生菌在宿主中组装和定植的机制。本副刊寻求支持 收集和维护一批鱿鱼，以完成与主要项目相关的研究，这些研究是不利的 受到涉及项目PI的重大人生事件的影响。拟议的活动将1)确定以下范围 在宿主相关细菌内表达一个小的调节RNA，以及2)决定如何进口一个 寄主来源的硫源在体内影响费氏弧菌的生理。定义的鱿鱼定殖率分析 细菌突变体将解决每个研究问题，从而完成两项研究。加在一起，这些 实验将提供有关费氏弧菌细胞是如何获得并适应鱿鱼宿主的知识。 环境，这将增加对细菌共生体如何成功建立的理解 与宿主共生。

PROJECT SUMMARY All animals depend on bacterial symbionts for specific traits that promote their fitness. The mechanisms by which hosts acquire and select for those symbionts remains unclear but important to develop new strategies and therapeutics based on bacteria to improve host health. The symbiosis established between the Hawaiian bobtail squid and the bioluminescent bacterium Vibrio fischeri is a powerful model to examine the molecular mechanisms that promote symbiont assembly and colonization in a host. This supplement seeks support to collect and maintain a cohort of squid to complete studies associated with the main project that were adversely affected by a critical life event involving the project PI. The proposed activities will 1) determine the extent of expression of a small regulatory RNA within host-associated bacteria, and 2) determine how the import of a host-derived sulfur source impacts V. fischeri physiology in vivo. Squid colonization assays with defined bacterial mutants will address each research question, thereby completing two studies. Together, these experiments will provide knowledge of how V. fischeri cells are acquired and acclimate to the squid host environment, which is insight that will increase understanding of how bacterial symbionts successfully establish symbiosis with a host.