Heterogeneity in bacterial quorum sensing during infection

感染过程中细菌群体​​感应的异质性

• 批准号: 10797383
• 负责人: Timothy Miyashiro
• 金额: $ 6.18万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10797383
• 关键词: Acclimatization; Affect; Animals; Bacteria; Biological Assay; Cells; Environment; Euprymna scolopes; Event; Hawaiian; Health; Heterogeneity; Human; Infection; Knowledge; Life; Modeling; Molecular; Physiology; RNA; Research; Signal Transduction; Source; Squid; Sulfur; Symbiosis; System; Therapeutic; Vibrio fischeri; beneficial microorganism; cohort; experimental study; fitness; gene regulatory network; improved; in vivo; insight; mutant; quorum sensing; symbiont; trait

PROJECT SUMMARY All animals depend on bacterial symbionts for specific traits that promote their fitness. The mechanisms by which hosts acquire and select for those symbionts remains unclear but important to develop new strategies and therapeutics based on bacteria to improve host health. The symbiosis established between the Hawaiian bobtail squid and the bioluminescent bacterium Vibrio fischeri is a powerful model to examine the molecular mechanisms that promote symbiont assembly and colonization in a host. This supplement seeks support to collect and maintain a cohort of squid to complete studies associated with the main project that were adversely affected by a critical life event involving the project PI. The proposed activities will 1) determine the extent of expression of a small regulatory RNA within host-associated bacteria, and 2) determine how the import of a host-derived sulfur source impacts V. fischeri physiology in vivo. Squid colonization assays with defined bacterial mutants will address each research question, thereby completing two studies. Together, these experiments will provide knowledge of how V. fischeri cells are acquired and acclimate to the squid host environment, which is insight that will increase understanding of how bacterial symbionts successfully establish symbiosis with a host.

项目摘要 所有动物都依赖于细菌共生体来获得促进其健康的特定特征。的机制 宿主获取和选择哪些共生体仍然不清楚，但对于开发新策略很重要 和基于细菌的治疗剂来改善宿主健康。夏威夷人和印第安人之间建立的共生关系 短尾鱿鱼和生物发光细菌费氏弧菌是一个强大的模型，以检查分子 促进共生体在宿主中组装和定殖的机制。本补编寻求支持， 收集和维持一批鱿鱼，以完成与主要项目有关的研究， 受涉及项目PI的重大生活事件影响。拟议的活动将：1）确定 在宿主相关细菌内的小调节RNA的表达，和2）确定如何输入一个 宿主来源的硫源影响费氏弧菌体内生理学。鱿鱼定殖测定， 细菌突变体将解决每个研究问题，从而完成两项研究。所有这些 实验将提供如何获得和适应鱿鱼宿主的V.fischeri细胞的知识 环境，这是洞察力，将增加了解细菌共生体如何成功地建立 与宿主共生

项目成果

Impact of transit time on the reproductive capacity of Euprymna scolopes as a laboratory animal.

• DOI: 10.1186/s42826-022-00135-2
• 发表时间: 2022-07-30
• 期刊: LABORATORY ANIMAL RESEARCH
• 影响因子: 2.9
• 作者: Cecere, Andrew G.;Miyashiro, Tim I.
• 通讯作者: Miyashiro, Tim I.

The type-VI secretion system of the beneficial symbiont Vibrio fischeri.

• DOI: 10.1099/mic.0.001302
• 发表时间: 2023-02
• 期刊: MICROBIOLOGY-SGM
• 影响因子: 2.8
• 作者: Guckes, Kirsten R.;Miyashiro, Tim I.
• 通讯作者: Miyashiro, Tim I.

Quantification of the capacity of vibrio fischeri to establish symbiosis with Euprymna scolopes.

• DOI: 10.1371/journal.pone.0287519
• 发表时间: 2023
• 期刊: PloS one
• 影响因子: 3.7

A case study assessing the impact of mating frequency on the reproductive performance of the Hawaiian bobtail squid Euprymna scolopes.

• DOI: 10.1186/s42826-023-00168-1
• 发表时间: 2023-07-28
• 期刊: LABORATORY ANIMAL RESEARCH
• 影响因子: 2.9
• 作者: Cecere, Andrew G.;Cook, Rachel A.;Miyashiro, Tim I.
• 通讯作者: Miyashiro, Tim I.