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Heterogeneity in bacterial quorum sensing during infection

感染过程中细菌群体感应的异质性

基本信息

批准号：
10217194
负责人：
Timothy Miyashiro
金额：
$ 31.96万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10217194
关键词：
Address Adolescent Animals Bacteria Bacterial Physiology Behavior Bioluminescence Bypass Cell physiology Cells Cellular Structures Cellular biology Data Detection Disease Environment Euprymna scolopes Exhibits Fluorescence Microscopy Gene Expression Gene Targeting Genes Genetic Transcription Goals Hawaiian population Health Heterogeneity Human In Vitro Individual Infection Knowledge Light Link Microbe Microbial Biofilms Microbial Genetics Molecular Molecular Biology Nature Organ Outcome Pathway interactions Pattern Phase Phenotype Physiological Physiology Population Process Production Property Regulation Regulator Genes Repression Research Signal Transduction Small RNA Squid Symbiosis System Testing Time Toxin Vibrio fischeri Virulence Work base beneficial microorganism cell behavior cell motility cell type design fitness hatching host colonization host-microbe interactions improved innovation insight intercellular communication juvenile animal novel pathogen quorum sensing tool trait

项目摘要

Quorum sensing describes the cell-signaling systems that facilitate intercellular interactions among bacteria and coordinate population-level behaviors. Quorum sensing typically alters cellular behavior by regulating gene expression. Bacteria also use quorum sensing to regulate factors important for infecting humans and other animals. There is a gap in understanding how quorum-sensing systems are integrated into the infection process. Addressing this knowledge gap is important to gain basic insight into how pathogens and beneficial microbes form stable host-microbe associations, which will inform strategies designed to control these associations through modulating quorum sensing. The overall objective of this proposal is to determine how the bacterium Vibrio fischeri uses gene-regulatory networks associated with quorum sensing during infection of its natural host, the Hawaiian squid. V. fischeri infections initially assemble within the light organ of juvenile animals, which are un-colonized at the time of hatching. V. fischeri uses quorum sensing to regulate bioluminescence, which is its symbiotic function within the squid host. The genes necessary for bioluminescence are regulated by quorum sensing. The small RNA Qrr1 is a component of this regulatory network and negatively controls bioluminescence. Guided by preliminary data, this proposal investigates how V. fischeri uses this network during host colonization by addressing three aims: 1) Understand regulation of qrr1 transcription in V. fischeri, 2) Examine V. fischeri aggregates for phenotypic heterogeneity, and 3) Evaluate how aggregation during symbiosis establishment impacts the quorum-sensing pathway. All three aims will take advantage of the innovative platform afforded by the symbiosis: fluorescence microscopy will be used to directly examine the infection process and molecular biology tools to dissect the associated quorum- sensing systems. The proposed research is significant because it will reveal important basic principles of how quorum-sensing networks function at different infection stages, which is knowledge that has broad applicability to other host-microbe interactions and provides critical insight into strategies designed to control these associations through modulating quorum sensing.

群体感应描述了促进细菌之间细胞间相互作用的细胞信号系统