The role of TRIM2 and SIRPA in New World Arenavirus entry
TRIM2 和 SIRPA 在新世界沙粒病毒进入中的作用
基本信息
- 批准号:10625278
- 负责人:
- 金额:$ 49.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-23 至 2027-04-30
- 项目状态:未结题
- 来源:
- 关键词:2019-nCoVActinsAerosolsAmino Acid MotifsAntiviral ResponseAntiviral TherapyApoptosisArenavirusArenavirus InfectionsArgentinaBindingBiological ProcessBlood PlateletsC-terminalCell Culture TechniquesCell ExtractsCell ProliferationCell Surface ProteinsCell surfaceCellsComplexCultured CellsCytoskeletonDataDiseaseEbolaEndocytosisEndotheliumErythrocytesFamilyFunctional disorderGenesGlycoproteinsGoalsHumanHuman PathologyImageImmune responseImmune systemIn VitroIncidenceInfectionInfection ControlIntegration Host FactorsJunin virusKnockout MiceLinkLymphocytic choriomeningitis virusMacrophageMediatingModelingMusMutationOld World ArenavirusesPTPN6 genePTPNS1 genePathogenicityPathway interactionsPatientsPhagocytosisPhagocytosis InhibitionPhosphoric Monoester HydrolasesPhosphorylationPrimatesProcessProtein FamilyProteinsRNA VirusesRNA interference screenRodentRoleSentinelSignal TransductionSignal Transduction PathwaySmall Interfering RNASpecificitySurvivorsTFRC geneTRIM MotifTacaribe Complex VirusesTacaribe virusTestingTherapeutic InterventionTissue ExtractsUbiquitinationVaccinesVesicular stomatitis Indiana virusViralViral Hemorrhagic FeversVirusVirus DiseasesVisualizationWorkZika VirusZoonosescell typecellular targetingcytokineexperimental studyin vivoinsightknock-downmembermortalityneoplastic cellnervous system disordernovelnovel strategiesnovel therapeutic interventionpathogenic viruspreventreceptortherapeutic targettherapeutically effectivetraffickingtransmission processtumorubiquitin ligaseviral transmission
项目摘要
New world hemorrhagic fever arenaviruses (NWAs), such as Junín virus, are rodent-transmitted
viruses that cause ~30% mortality when they zoonose into humans. The mechanism by the NWAs
induce disease is still not certain, although it likely includes induction of high levels of cytokines
by infected sentinel cells of the immune system, leading to endothelia and thrombocyte
dysfunction and neurological disease. Survivors of Junín infection develop strong humoral
immune responses, suggesting that controlling infection at early times post-infection is critical for
virus clearance. Although an effective Junín virus vaccine has decreased disease incidence,
sporadic cases of this as well as the other known and novel NWAs for which there are no vaccines
or effective therapeutics still occur. It is well-established that the clade B pathogenic NWAs bind
to transferrin receptor 1 and other receptors on the cell surface, but the steps leading to their entry
from an acidic cellular compartment are not well-determined. We recently performed a siRNA
screen with pseudotyped viruses bearing a pathogenic Junín glycoprotein with the goal of finding
host genes involved in entry that could serve as therapeutic targets. We found that TRIM2, a
member of the tripartite motif family that includes well-known members of the host's intrinsic
defense against viral infections, limits NWA endocytosis into cells. By probing the TRIM2
interactome for other host proteins that block NWA infection, we discovered that SIRPA, a cell
surface protein that inhibits macrophage phagocytosis of tumor and dead cells and erythrocytes,
also decreases infection. Importantly, SIRPA, unlike TRIM2, inhibits infection by various human
pathogenic viruses that require trafficking to an acidic compartment, including VSV, Zika virus,
LCMV and Ebola and SARS-Cov-2 pseudoviruses. Our data suggest that TRIM2 and SIRPA act
at the viral entry/internalization step. These finding suggests that there are common mechanisms
that regulate virus endocytosis and phagocytosis.
We propose here to further investigate the overlap between virus-mediated endocytosis
and phagocytosis in vitro, ex vivo and in vivo in three aims that will 1) investigate the overlap in
the NWA entry and phagocytosis pathways; 2) determine where TRIM2/SIRPA inhibition of
infection occurs; and 3) use TRIM2, SIRPA and other relevant knockout mice to probe the roles
of these proteins in cell-type specific and in vivo infection by replication-competent NWAs. In
addition to providing mechanistic insight into the entry of NWAs into cells, these studies have the
potential of increasing our understanding as to how host factors limit infection and could lead to
new approaches to therapeutic intervention.
新世界出血热沙粒病毒(NWA),如胡宁病毒,是啮齿动物传播的
当它们通过人畜共患病传染给人类时会导致约30%的死亡率。NWA的机制
诱导疾病仍然不确定,尽管它可能包括诱导高水平细胞因子
免疫系统的哨兵细胞感染,导致内皮细胞和血小板
功能障碍和神经系统疾病。Junín感染的幸存者产生强烈的体液免疫
免疫反应,这表明在感染后的早期控制感染对于
病毒清除虽然有效的胡宁病毒疫苗降低了发病率,
这种疾病以及其他已知的和新型的无疫苗的NWA的零星病例
或有效的治疗方法仍然存在。已经确定进化枝B致病性NWA结合
转铁蛋白受体1和细胞表面的其他受体,但导致它们进入的步骤
来自酸性细胞隔室的物质还不能很好地确定。我们最近做了一个siRNA
用携带致病性Junín糖蛋白的假型病毒进行筛选,目的是发现
参与进入的宿主基因可以作为治疗靶点。我们发现TRIM 2,
三重基序家族的成员,包括宿主固有的已知成员
针对病毒感染的防御,限制NWA内吞进入细胞。通过探测TRIM 2
对于其他阻断NWA感染的宿主蛋白质的相互作用组,我们发现SIRPA,一种细胞,
抑制巨噬细胞吞噬肿瘤、死细胞和红细胞的表面蛋白,
也减少了感染。重要的是,SIRPA与TRIM 2不同,抑制各种人类感染。
需要运输到酸性隔室的致病病毒,包括VSV、寨卡病毒,
LCMV和埃博拉和SARS-Cov-2假病毒。我们的数据表明TRIM 2和SIRPA作用于
在病毒进入/内化步骤。这些发现表明,
调节病毒的内吞作用和吞噬作用。
我们在此建议进一步研究病毒介导的内吞作用
和吞噬作用在体外,离体和体内的三个目标,将1)调查重叠,
NWA进入和吞噬途径; 2)确定TRIM 2/SIRPA抑制
利用TRIM 2、SIRPA等相关基因敲除小鼠探讨其在感染中的作用
这些蛋白质在细胞类型特异性和体内感染的复制能力NWA。在
除了提供NWA进入细胞的机制见解外,这些研究还具有
增加我们对宿主因素如何限制感染并可能导致
治疗干预的新方法。
项目成果
期刊论文数量(0)
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SUSAN R ROSS其他文献
SUSAN R ROSS的其他文献
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{{ truncateString('SUSAN R ROSS', 18)}}的其他基金
Interplay between reverse transcription and host restriction
逆转录与宿主限制之间的相互作用
- 批准号:
10607086 - 财政年份:2023
- 资助金额:
$ 49.38万 - 项目类别:
The role of TRIM2 and SIRPA in New World Arenavirus entry
TRIM2 和 SIRPA 在新世界沙粒病毒进入中的作用
- 批准号:
10362439 - 财政年份:2022
- 资助金额:
$ 49.38万 - 项目类别:
Role of DDX41 in HSC development and MDS/AML
DDX41 在 HSC 发育和 MDS/AML 中的作用
- 批准号:
10216402 - 财政年份:2021
- 资助金额:
$ 49.38万 - 项目类别:
Role of DDX41 in HSC development and MDS/AML
DDX41 在 HSC 发育和 MDS/AML 中的作用
- 批准号:
10373097 - 财政年份:2021
- 资助金额:
$ 49.38万 - 项目类别:
Role of APOBEC3 in in vivo Restriction of Retrovirus Infection
APOBEC3 在体内限制逆转录病毒感染中的作用
- 批准号:
9054058 - 财政年份:2016
- 资助金额:
$ 49.38万 - 项目类别:
Role of DNA sensors in host anti-retroviral defense
DNA传感器在宿主抗逆转录病毒防御中的作用
- 批准号:
9172791 - 财政年份:2016
- 资助金额:
$ 49.38万 - 项目类别:
Role of APOBEC3 in in vivo Restriction of Retrovirus Infection
APOBEC3 在体内限制逆转录病毒感染中的作用
- 批准号:
9176518 - 财政年份:2016
- 资助金额:
$ 49.38万 - 项目类别:
APOBEC3-mediated damage of host genomic DNA in vivo
APOBEC3 介导的体内宿主基因组 DNA 损伤
- 批准号:
8822043 - 财政年份:2015
- 资助金额:
$ 49.38万 - 项目类别:
Gordon Research Conference on "Infections of the nervous system: Pathogenesis and Worldwide Impact1"
戈登研究会议“神经系统感染:发病机制和全球影响1”
- 批准号:
8986297 - 财政年份:2015
- 资助金额:
$ 49.38万 - 项目类别:
TRIM2, a novel host factor that restricts New World Arenavirus infection
TRIM2,一种限制新世界沙粒病毒感染的新型宿主因子
- 批准号:
8852547 - 财政年份:2014
- 资助金额:
$ 49.38万 - 项目类别:
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