Neural coding of leg proprioception

腿部本体感觉的神经编码

• 批准号: 10624896
• 负责人: John Tuthill
• 金额: $ 38.55万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10624896
• 关键词: 3-Dimensional; Anatomy; Animals; Award; Axon; Behavior; Behavioral; Calcium; Central Nervous System; Code; Computer Models; Development; Drosophila genus; Drosophila melanogaster; Electrophysiology (science); Feedback; Genetic; Genetic Models; Health; Human; Image; Interneurons; Invertebrates; Joints; Label; Leg; Limb structure; Maps; Measures; Methods; Modeling; Morphology; Motor; Movement; Movement Disorders; Muscle Spindles; Musculoskeletal Equilibrium; Nerve; Neurons; Patients; Peripheral; Phase; Positioning Attribute; Posture; Process; Proprioception; Proprioceptor; Research; Role; Scheme; Signal Transduction; Site; Somatosensory Disorders; Specificity; Speed; Spinal Cord; Synapses; System; Therapeutic; Vertebrates; Walking; arm; arm movement; body position; cell type; chronic pain; flexibility; fly; improved; innovation; insight; kinematics; limb movement; mechanical force; model organism; motor behavior; motor control; neural; neuromechanism; optogenetics; peripheral nerve damage; presynaptic; response; sensor; sensory feedback; somatosensory; synaptic inhibition; tool; two-photon; vibration

Project Summary Proprioception, the sense of self-movement and body position, is critical for the effective control of motor behavior. Humans lacking proprioceptive feedback, such as patients with peripheral nerve damage, are unable to maintain limb posture or coordinate fine-scale movements of the arms and legs. But despite the importance of proprioception to the control of movement in all animals, little is known about the neural computations that underlie limb proprioception in any animal. This gap is due to two basic challenges: (1) identifying specific neuronal-cell types that detect and process proprioceptive signals, and (2) recording neural activity from proprioceptive circuits during natural limb movements. Here, we propose to overcome these challenges by investigating the neural coding of leg proprioception in a genetic model organism: the fruit fly, Drosophila. We have developed new methods to record and manipulate the activity of genetically- identified neurons in proprioceptive circuits of behaving flies. We will first determine the functional role of distinct proprioceptor subtypes in controlling limb posture and movement during walking (Aim 1). We will then compare how proprioceptive signals are encoded during passive and active limb movements (Aim 2), and trace down the circuit mechanisms that underlie state-dependent proprioceptive coding (Aim 3). Altogether, these studies will elucidate basic mechanisms of proprioceptive neural processing that have not possible to investigate in other systems. Although there are morphological differences between flies and humans, the basic building blocks of invertebrate and vertebrate somatosensory systems share a striking evolutionary conservation. These similarities suggest that the general principles discovered in circuits of the fruit fly will be highly relevant to somatosensory processing in other animals. A deeper understanding of proprioception has the potential to transform the way in which we treat somatosensory disorders.

项目摘要 本体感觉，即对自我运动和身体位置的感觉，对于有效控制至关重要 运动行为。缺乏本体感受反馈的人，例如外周神经系统疾病患者， 神经损伤，无法保持肢体姿势或协调的精细规模的运动， 胳膊和腿但是，尽管本体感觉对所有人的运动控制都很重要， 动物，很少有人知道神经计算的基础肢体本体感觉在任何 动物这一差距是由于两个基本的挑战：（1）确定特定的神经细胞类型， 检测和处理本体感受信号，以及（2）记录来自本体感受信号的神经活动。 自然肢体运动时的回路在这里，我们建议通过以下方式克服这些挑战 在遗传模式生物果蝇中研究腿部本体感觉的神经编码， 果蝇我们已经开发出新的方法来记录和操纵基因的活动- 在果蝇的本体感受回路中发现了神经元。我们将首先确定 不同的本体感受器亚型在控制肢体姿势和运动中的功能作用 行走时（目标1）。然后，我们将比较本体感受信号是如何编码， 被动和主动肢体运动（目标2），并追查电路机制， 状态依赖性本体感受编码（Aim 3）。总之，这些研究将阐明基本的 本体感受神经处理的机制，还没有可能在其他研究中进行研究。 系统.虽然苍蝇和人类在形态上存在差异， 无脊椎动物和脊椎动物躯体感觉系统的组成部分有一个惊人的共同点， 进化保守这些相似之处表明， 果蝇的神经回路将与其他动物的躯体感觉处理高度相关。一 对本体感受的深入理解有可能改变我们治疗 躯体感觉障碍

项目成果

Anipose: A toolkit for robust markerless 3D pose estimation.

• DOI: 10.1016/j.celrep.2021.109730
• 发表时间: 2021-09-28
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Karashchuk P;Rupp KL;Dickinson ES;Walling-Bell S;Sanders E;Azim E;Brunton BW;Tuthill JC
• 通讯作者: Tuthill JC

Dense neuronal reconstruction through X-ray holographic nano-tomography.

• DOI: 10.1038/s41593-020-0704-9
• 发表时间: 2020-12
• 期刊: Nature neuroscience
• 影响因子: 25
• 作者: Kuan AT;Phelps JS;Thomas LA;Nguyen TM;Han J;Chen CL;Azevedo AW;Tuthill JC;Funke J;Cloetens P;Pacureanu A;Lee WA
• 通讯作者: Lee WA

Neural Coding of Leg Proprioception in Drosophila.

果蝇中腿部本体感受的神经编码。

• DOI: 10.1016/j.neuron.2018.09.009
• 发表时间: 2018-11-07
• 期刊: Neuron
• 影响因子: 16.2
• 作者: Mamiya A;Gurung P;Tuthill JC
• 通讯作者: Tuthill JC