Improvements to the Regional Biocontainment Research Facilities at the University of Missouri

母公司G20赠款的目标是提供最先进、高度安全和可靠的 生物遏制设施和服务，以教师，学生和工作人员的研究人员在MU实验室， 传染病，12个NIH/NIAID区域生物防护实验室之一。LIDR/MURBL 目前有60多名研究人员。我们的研究社区是成功的赠款资金与14 主要研究人员目前积极参与联邦和私人资助的赠款/合同。目前ABSL 3 设施支持啮齿动物的气溶胶挑战，但由于缺乏 合适的密封外壳。这个G20补充项目的目标是扩大 MURBL为该地区的生物防御和新兴传染病研究服务。为实现这一 目标，我们建议翻新ABSL 3的东套件，包括安装一个专门的 前厅和污水净化系统，以允许在生物防护中饲养大型动物。 这些升级将满足RBL的MU教师的迫切需要，他们正在进行 SARS-CoV-2、流感病毒和B型肝炎病毒的转化研究。 目标1：为LIDR的ABSL 3增加开放式笼舍功能，用于饲养猪和其他大型动物 在用对人类致病的呼吸道病毒攻击后。我们建议建立开放的 LIDR的ABSL 3的笼养能力，可容纳多达10头猪或其他大型动物， 实验性感染了各种人类病原体该请求包括改建 接待室以及动物收容室/建筑物污水系统，以允许污水净化，以及 必要的HVAC升级。 目标2：购买和安装感染后整体动物成像设备。我们建议加入 整个动物成像的能力，这将允许减少在LIDR使用的研究动物。

The objective of the parent G20 grant is to provide state of the art, highly safe and secure biocontainment facilities and services to faculty, student and staff researchers at the MU Laboratory for Infectious Diseases, one of 12 NIH/NIAID Regional Biocontainment Laboratories. The LIDR/MURBL is currently host to more than 60 researchers. Our research community is successful in grant funding with 14 Principal Investigators currently active in federally and privately funded grants/contracts. The current ABSL3 facility supports aerosol challenge of rodents, but larger animal challenges cannot occur due to a lack of appropriate containment housing. The goal of this G20 supplement project is to expand the capabilities of the MURBL to serve the biodefense and emerging infectious disease research for the region. Towards this objective, we propose to renovate the east suite of the ABSL3, including the installation of a specialized ante room and effluent decontamination system, to allow for the housing of large animals in biocontainment. These upgrades would serve the immediate needs of MU faculty of the RBL who are conducting translational research on SARS-CoV-2, influenza virus, and hepatitis B virus. Aim 1: Add open caging capabilities to the ABSL3 at LIDR for housing swine and other large animals following challenge with respiratory viruses that are pathogenic to humans. We propose to build open caging capacity in the ABSL3 at LIDR for housing up to 10 pigs or other large animals that have been experimentally infected with various human pathogens. The request includes funds for remodeling in the anteroom as well as the animal holding room/building effluent system to permit effluent decontamination, and necessary HVAC upgrades. Aim 2: Purchase and install equipment for whole animal imaging following infection. We propose to add capabilities for whole animal imaging, which will allow for reduction of research animals used at LIDR.