Specific Pathogen Free 18 Baboon Research Resource (SPF18BRR): Applied Research

无特定病原体 18 狒狒研究资源 (SPF18BRR)：应用研究

• 批准号: 10627138
• 负责人: Joe H. Simmons
• 金额: $ 6.05万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627138
• 关键词: 15 year old; 3 year old; Adult; Age; Aging; Alzheimer' s disease related dementia; Animal Model; Animals; Applications Grants; Applied Research; Biological Markers; Biomedical Research; Breeding; Cerebrospinal Fluid; Cognition; DNA Methylation; Diabetes Mellitus; Doctor of Philosophy; Epigenetic Process; Female; Foundations; Genetic; Human; Intramural Research Program; Measures; Neurosciences; Papio; Papio anubis; Pharmacologic Substance; Plasma; Population Genetics; Primates; Privatization; Research; Research Contracts; Resources; System; Training; United States National Institutes of Health; Virus; age group; animal breeding; cognitive testing; genome sequencing; germ free condition; human disease; human model; improved; male; model development; nonhuman primate; offspring; pathogen; social; whole genome

PROJECT SUMMARY: APPLIED RESEARCH COMPONENT The SPF18BRR is the only national research resource of adventitious virus free olive baboons (Papio anubis) that are available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPF18BRR have an extensive bioexclusion list of 18 pathogens normally found in other wild and captive nonhuman primate colonies. The applied research proposed in this grant application has been developed to strengthen and improve the resource. The first two specific aims are intended to further develop the resource and to demonstrate the value of the SPF18 baboons as an animal model of human Alzheimer’s disease and related dementias (AD/ADRD). Specific aim one will develop and place hardware for automated cognitive testing in socially living baboons. Specific aim two expands on the AD/ADRD model development by calculating a DNA methylation clock for baboons in the SPF18BRR and by measuring a panel of 30 biomarkers on all animals 3 years of age and older at two different timepoints. Additionally, we will collect cerebrospinal fluid from animals 15 years of age and older and will use it to measure a panel of 18 human neuroscience biomarkers. In specific aim three we will perform whole genome sequencing (WGS) on nine olive baboons that were bred at the Southwest National Primate Research Center that we would like to add to our conventional baboon breeding colony to diversify the population genetics before we close the SPF18 colony to new animal introductions. We will also perform targeted WGS on additional animals to further expand the understanding of the genetics of the SPF18BRR.

项目摘要：应用研究部分 SPF 18 BRR是唯一的无外源病毒橄榄狒狒（Papio anubis）的国家研究资源 可供NIH资助者使用;联邦机构的内部研究计划，包括FDA，NSF 和NIH;以及其他生物医学研究赞助商（私人基金会，制药公司， 合同研究组织）。绝对独一无二的在整个世界上，狒狒在SPF 18 BRR有 一份广泛的生物排斥清单，列出了通常在其他野生和圈养的非人灵长类动物中发现的18种病原体 殖民地在这项拨款申请中提出的应用研究已经发展，以加强和 改善资源。前两个具体目标旨在进一步开发资源， 证明SPF 18狒狒作为人类阿尔茨海默病和相关疾病的动物模型的价值 痴呆（AD/ADRD）。具体目标之一是开发和放置自动认知测试的硬件， 群居狒狒具体目标二通过计算DNA扩展了AD/ADRD模型的开发 在SPF 18 BRR中，通过测量狒狒的甲基化时钟，并通过测量所有动物的30种生物标志物3 在两个不同的时间点年龄和年龄更大。此外，我们将收集脑脊液， 15岁及以上的动物，并将使用它来测量一组18个人类神经科学生物标志物。 在第三个具体目标中，我们将对九只繁殖的橄榄狒狒进行全基因组测序（WGS）。 在西南国家灵长类动物研究中心，我们想把它加入到我们传统的狒狒中， 在我们关闭SPF 18群体之前，我们需要繁殖群体，以使群体遗传学多样化 自我介绍我们还将对其他动物进行有针对性的WGS，以进一步扩大了解 SPF 18 BRR的基因。