Specific Pathogen Free 18 Baboon Research Resource (SPF18BRR): Applied Research

项目摘要：应用研究部分 SPF18BRR是国家唯一的不定病毒脱毒橄榄狒狒(Papio Anubis)研究资源 可供NIH受赠者使用；联邦机构的内部研究计划，包括FDA、NSF 和NIH；以及其他生物医学研究赞助商(私人基金会、制药公司和 合同研究机构)。在整个世界上绝对是独一无二的，SPF18BRR中的狒狒拥有 广泛的生物排斥清单，列出了通常在其他野生和圈养非人类灵长类动物中发现的18种病原体 殖民地。在这项赠款申请中提出的应用研究已被开发，以加强和 优化资源配置。前两个具体目标旨在进一步开发资源和 证明SPF18狒狒作为人类阿尔茨海默病及相关动物模型的价值 痴呆症(AD/ADRD)。具体目标一将开发和放置用于自动化认知测试的硬件 群居生活的狒狒。专门目的二通过计算DNA来扩展AD/ADRD模型的开发 通过测量所有动物上的30个生物标记物，研究了SPF18BRR中狒狒的甲基化时钟3 年龄和年龄在两个不同的时间点。此外，我们还将从 15岁及以上的动物，并将用它来测量由18个人类神经科学生物标志物组成的小组。 在具体目标三中，我们将对培育出的9只橄榄狒狒进行全基因组测序 在西南国家灵长类动物研究中心，我们想把它添加到我们传统的狒狒中 在我们向新动物关闭SPF18群体之前，培育群体以使群体遗传学多样化 自我介绍。我们还将对更多的动物进行有针对性的WGS，以进一步扩大了解 SPF18BRR的遗传学。

PROJECT SUMMARY: APPLIED RESEARCH COMPONENT The SPF18BRR is the only national research resource of adventitious virus free olive baboons (Papio anubis) that are available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPF18BRR have an extensive bioexclusion list of 18 pathogens normally found in other wild and captive nonhuman primate colonies. The applied research proposed in this grant application has been developed to strengthen and improve the resource. The first two specific aims are intended to further develop the resource and to demonstrate the value of the SPF18 baboons as an animal model of human Alzheimer’s disease and related dementias (AD/ADRD). Specific aim one will develop and place hardware for automated cognitive testing in socially living baboons. Specific aim two expands on the AD/ADRD model development by calculating a DNA methylation clock for baboons in the SPF18BRR and by measuring a panel of 30 biomarkers on all animals 3 years of age and older at two different timepoints. Additionally, we will collect cerebrospinal fluid from animals 15 years of age and older and will use it to measure a panel of 18 human neuroscience biomarkers. In specific aim three we will perform whole genome sequencing (WGS) on nine olive baboons that were bred at the Southwest National Primate Research Center that we would like to add to our conventional baboon breeding colony to diversify the population genetics before we close the SPF18 colony to new animal introductions. We will also perform targeted WGS on additional animals to further expand the understanding of the genetics of the SPF18BRR.