Specific Pathogen Free Baboon Research Resource (SPFBRR) - A&R Administrative Supplement

项目摘要 无特定病原菌猴研究资源(SPFBRR)是全国唯一 NIH受资助者可使用的SPF18狒狒研究资源；校内研究计划 联邦机构，包括FDA、NSF和NIH；以及生物医学的其他赞助商 研究(私人基金会、制药公司和合同研究组织)。 绝对是世界上独一无二的，SPFBRR中的橄榄狒狒(Papio Anubis)有一种 广泛的生物排除清单，列出了通常在野生和圈养狒狒群体中发现的18种病原体。 事实上，来自SPFBRR的狒狒没有所有已知的不定病毒，这些病毒通常 感染野生和圈养的狒狒。在接下来的几年里，一个主要的挑战 将殖民地迁至基林中心一直在发展广泛的曲目 确保SPF18动物保持自由的血清学和PCR检测分析和策略 排除的病原体。这份A&R行政副刊将允许我们购买两个 设备：Bio-Plex 200系统用于开发室内狒狒专用多路复用器 血清学检测和QX200ddPCR系统阐明早期病毒的发病机制 幼稚的橄榄狒狒的感染。这项请求直接涉及P40的具体目标2 扩大育种资源的资源核心和研究核心的具体目标1 发展分子诊断分析来支持SPFBRR。建议的研究是在 父级奖励的范围，并将刺激其他活动，从而推动 家长奖的总体目标。拟议的研究与已经获得资金的研究没有重叠。 在家长奖中工作。

Project Summary The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF18 baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the world, the olive baboons (Papio anubis) in the SPFBRR have an extensive bioexclusion list of 18 pathogens normally found in wild and captive baboon colonies. In fact, baboons from the SPFBRR are free of all known adventitious viruses that commonly infect wild and captive baboons. A major challenge during the first few years following relocation of the colony to the Keeling Center has been developing the extensive repertoire of serology and PCR testing assays and strategies to ensure that the SPF18 animals remain free of excluded pathogens. This A&R Administrative Supplement will allow us to purchase two pieces of equipment: a Bio-Plex 200 System to develop inhouse baboon specific multiplexed serology assays, and a QX200 ddPCR System elucidate the pathogenesis of incipient virus infections in naïve olive baboons. This request directly addresses Specific Aim 2 of the P40 Resource Core to expand the breeding resource and Specific Aim 1 of the Research Core to develop molecular diagnostic assays to support the SPFBRR. The proposed study is within the scope of the parent award and will stimulate additional activities leading to the progress of the overall goals of the parent award. The proposed study does not overlap with already funded work in the parent award.