Specific Pathogen Free Baboon Research Resource (SPFBRR) - A&R Administrative Supplement

项目摘要 无特定病原体狒狒研究资源（SPFBRR）是唯一的国家 国家卫生研究院受资助者可利用的SPF 18狒狒研究资源;校内研究计划 包括FDA、NSF和NIH在内的联邦机构;以及其他生物医学 研究（私人基金会、制药公司和合同研究组织）。 绝对是世界上独一无二的，在SPFBRR的橄榄狒狒（Papio anubis）有一个 广泛的生物排斥清单，列出了通常在野生和圈养狒狒群体中发现的18种病原体。 事实上，来自SPFBRR的狒狒没有所有已知的外来病毒， 感染野生和圈养狒狒。一个主要的挑战，在最初几年后， 将殖民地搬迁到基林中心， 血清学和PCR检测分析和策略，以确保SPF 18动物保持自由 排除的病原体。这份A&R行政补充协议将允许我们购买两份 设备：一个Bio-Bio 200系统，用于开发内部狒狒特异性多重 血清学试验和QX 200 ddPCR系统阐明了早期病毒的发病机制 感染了幼稚的橄榄狒狒。这一要求直接涉及P40的具体目标2 扩大育种资源的资源核心和研究核心的具体目标1 开发分子诊断分析以支持SPFBRR。拟议的研究是在 的范围，并将刺激更多的活动，导致进展的 家长奖励的总体目标。拟议的研究与已资助的研究不重叠。 在家长奖工作。

Project Summary The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF18 baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the world, the olive baboons (Papio anubis) in the SPFBRR have an extensive bioexclusion list of 18 pathogens normally found in wild and captive baboon colonies. In fact, baboons from the SPFBRR are free of all known adventitious viruses that commonly infect wild and captive baboons. A major challenge during the first few years following relocation of the colony to the Keeling Center has been developing the extensive repertoire of serology and PCR testing assays and strategies to ensure that the SPF18 animals remain free of excluded pathogens. This A&R Administrative Supplement will allow us to purchase two pieces of equipment: a Bio-Plex 200 System to develop inhouse baboon specific multiplexed serology assays, and a QX200 ddPCR System elucidate the pathogenesis of incipient virus infections in naïve olive baboons. This request directly addresses Specific Aim 2 of the P40 Resource Core to expand the breeding resource and Specific Aim 1 of the Research Core to develop molecular diagnostic assays to support the SPFBRR. The proposed study is within the scope of the parent award and will stimulate additional activities leading to the progress of the overall goals of the parent award. The proposed study does not overlap with already funded work in the parent award.