Specific Pathogen Free Baboon Research Resource (SPFBRR)

总体-项目概要/摘要 无特定病原体狒狒研究资源（SPFBRR）是唯一的国家研究资源 的SPF狒狒提供给国家卫生研究院赠款;内部研究计划的联邦机构，包括 FDA、NSF和NIH;以及生物医学研究的其他赞助商（私人基金会、制药公司、 公司和合同研究组织）。世界上独一无二的狒狒 SPFBRR有一个广泛的生物排斥名单，其中包括19种通常在其他野生和圈养动物中发现的病原体。 非人类灵长类动物群落SPFBRR最初是作为R24开发的，后来又作为P40开发的， 俄克拉荷马州大学健康科学中心（OUHSC）和俄克拉荷马州州立大学之间的合作 大学（OSU）。然而，最近决定结束所有非人类灵长类动物的研究在OUHSC 需要努力重新安置SPFBRR。OUHSC领导层的详尽努力导致了 为这一宝贵而独特的国家研究资源确定一个新的家园。该Michale E. Keeling Center for Comparative Medicine and Research（Keeling Center）德克萨斯大学医学博士 位于德克萨斯州巴斯特罗普的安德森癌症中心拥有设施、专业知识、经验和基础设施， SPFBRR的当前和未来需求。在这项拨款申请中，我们建议将 到基林中心，通过从一只狒狒中重新培育狒狒幼崽来加速SPF群体的生长。 常规（非SPF）繁殖群体，并保持和扩大与研究计划的伙伴关系， 这些珍贵的SPF狒狒资源在本申请所要求的支持期间， SPFBRR将扩大，以创建一个自我维持的SPF繁殖群体， 基因强健的繁殖动物这将消除婴儿从常规（非SPF） 这将显著降低将外源性感染因子引入SPFBRR的风险。 该P40应用程序的总体目标是将SPFBRR迁移到基林中心， 可以保护不可或缺国家研究资源;向科学家提供教育和培训， 兽医，殖民地管理者和与狒狒一起工作的动物护理人员;进行应用研究， 改善资源;以及支持需要研究项目的设施和专业知识的研究人员 涉及SPF狒狒。我们还将进行主要组织相容性复合物的mRNA测序， (MHC)在SPFBRR中所有基础繁殖狒狒的I类等位基因;并且，我们将执行整个 对资源中的26只动物进行基因组测序（WGS），以进行基因组和谱系分析。这些信息 将进一步完善和增值SPFBRR提供的资源。设施最初设计用于 黑猩猩将被重新利用，以满足SPFBRR和基林其他研究设施的需要。 中心将提供空间进行研究与SPF狒狒。

OVERALL – Project Summary/Abstract The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPFBRR have an extensive bioexclusion list of 19 pathogens normally found in other wild and captive nonhuman primate colonies. The SPFBRR was originally developed as an R24, and later as a P40, grant in a collaboration between the University of Oklahoma Health Sciences Center (OUHSC) and Oklahoma State University (OSU). However, a recent decision to end all nonhuman primate research at OUHSC has necessitated efforts to relocate the SPFBRR. An exhaustive effort by the leadership of OUHSC has resulted in the identification of a new home for this valuable and unique national research resource. The Michale E. Keeling Center for Comparative Medicine and Research (Keeling Center) of The University of Texas MD Anderson Cancer Center in Bastrop, Texas has the facilities, expertise, experience, and infrastructure to meet the current and future needs of the SPFBRR. With this grant application, we propose to relocate the SPFBRR to the Keeling Center, accelerate growth of the SPF colony through re-derivation of infant baboons from a conventional (non-SPF) breeding colony, and maintain and expand partnerships with research programs using these invaluable SPF baboon resources. During the period of support requested in this application, the SPFBRR will be expanded to create a self-sustaining SPF breeding colony of behaviorally healthy and genetically robust breeding animals. This will eliminate the need for infants from conventional (non-SPF) dams, and it will significantly decrease the risk of introducing adventitious infectious agents into the SPFBRR. The overall goals of this P40 application are to relocate the SPFBRR to the Keeling Center so that this indispensable national research resource can be preserved; provide education and training to scientists, veterinarians, colony managers, and animal caregivers who work with baboons; conduct applied research to improve the resource; and, support investigators who need facilities and expertise for research projects involving SPF baboons. We will also perform mRNA sequencing of the major histocompatability complex (MHC) class I alleles for all of the foundation breeding baboons in the SPFBRR; and, we will perform whole genome sequencing (WGS) on 26 animals in the resource for genomic and pedigree analysis. This information will further refine and add value to the resources provided by the SPFBRR. Facilities originally designed for chimpanzees will be repurposed to meet the needs of the SPFBRR and other research facilities at the Keeling Center will provide space to conduct research with SPF baboons.