Specific Pathogen Free Baboon Research Resource (SPFBRR)

无特定病原体狒狒研究资源 (SPFBRR)

• 批准号: 9545092
• 负责人: Joe H. Simmons
• 金额: $ 115.01万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9545092
• 关键词: Alleles; Animal Experimentation; Animals; Applications Grants; Applied Research; Area; Arterivirus; Behavioral; Biocompatible Materials; Biological Assay; Biomedical Research; Bordetella; Breeding; Caregivers; Childhood; Collaborations; Complex; Derivation procedure; Development; Disease; Equipment; Evaluation; Female; Foundations; Future; Generations; Genes; Genetic Variation; Genomics; Goals; Grant; Growth; Health; Health Sciences; Herpesviridae; Heterozygote; Home environment; Human; Infant; Infection; Infectious Agent; Intramural Research Program; Laboratories; Laboratory Animals; Leadership; Length; MHC Class I Genes; Maternal antibody; Medicine; Monitor; Monoclonal Antibody R24; Nurseries; Oklahoma; Pan Genus; Papio; Pertussis; Pharmacologic Substance; Phase; Pilot Projects; Privatization; Production; Quantitative Reverse Transcriptase PCR; Research; Research Contracts; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Respiratory syncytial virus; Retroviridae; Risk; Scheme; Scientist; Septic Shock; Serologic tests; Site; Testing; Texas; Training; Training and Education; Translational Research; United States National Institutes of Health; Universities; University of Texas M D Anderson Cancer Center; Vaccinated; Veterinarians; Virus; Work; Xenograft procedure; animal breeding; animal resource; comparative; design; diagnostic assay; exhaustion; experience; genetic pedigree; genome sequencing; germ free condition; human disease; improved; mRNA sequencing; male; molecular diagnostics; next generation; nonhuman primate; novel; novel virus; offspring; pathogen; pre-clinical research; programs; research facility; whole genome

OVERALL – Project Summary/Abstract The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPFBRR have an extensive bioexclusion list of 19 pathogens normally found in other wild and captive nonhuman primate colonies. The SPFBRR was originally developed as an R24, and later as a P40, grant in a collaboration between the University of Oklahoma Health Sciences Center (OUHSC) and Oklahoma State University (OSU). However, a recent decision to end all nonhuman primate research at OUHSC has necessitated efforts to relocate the SPFBRR. An exhaustive effort by the leadership of OUHSC has resulted in the identification of a new home for this valuable and unique national research resource. The Michale E. Keeling Center for Comparative Medicine and Research (Keeling Center) of The University of Texas MD Anderson Cancer Center in Bastrop, Texas has the facilities, expertise, experience, and infrastructure to meet the current and future needs of the SPFBRR. With this grant application, we propose to relocate the SPFBRR to the Keeling Center, accelerate growth of the SPF colony through re-derivation of infant baboons from a conventional (non-SPF) breeding colony, and maintain and expand partnerships with research programs using these invaluable SPF baboon resources. During the period of support requested in this application, the SPFBRR will be expanded to create a self-sustaining SPF breeding colony of behaviorally healthy and genetically robust breeding animals. This will eliminate the need for infants from conventional (non-SPF) dams, and it will significantly decrease the risk of introducing adventitious infectious agents into the SPFBRR. The overall goals of this P40 application are to relocate the SPFBRR to the Keeling Center so that this indispensable national research resource can be preserved; provide education and training to scientists, veterinarians, colony managers, and animal caregivers who work with baboons; conduct applied research to improve the resource; and, support investigators who need facilities and expertise for research projects involving SPF baboons. We will also perform mRNA sequencing of the major histocompatability complex (MHC) class I alleles for all of the foundation breeding baboons in the SPFBRR; and, we will perform whole genome sequencing (WGS) on 26 animals in the resource for genomic and pedigree analysis. This information will further refine and add value to the resources provided by the SPFBRR. Facilities originally designed for chimpanzees will be repurposed to meet the needs of the SPFBRR and other research facilities at the Keeling Center will provide space to conduct research with SPF baboons.

总体 - 项目摘要/摘要 特定的无病原体研究资源（SPFBRR）是唯一的国家研究资源 NIH Grandes可用的SPF狒狒；联邦机构的壁内研究计划，包括 FDA，NSF和NIH；以及其他生物医学研究的赞助商（私人基金会，药物 公司和合同研究组织）。在全世界绝对独特，狒狒 SPFBRR在其他野外和俘虏中发现了19种病原体的广泛生物排斥列表 非人类灵长类动物殖民地。 SPFBRR最初是作为R24开发的，后来以P40的形式开发 俄克拉荷马大学健康科学中心（OUHSC）与俄克拉荷马州立大学之间的合作 大学（OSU）。但是，最近决定结束OUHSC的所有非人类私人研究的决定 有必要努力搬迁SPFBRR。 OUHSC领导的详尽努力导致 为这一宝贵而独特的国家研究资源确定新家。迈克尔E. 德克萨斯大学医学博士Keeling比较医学与研究中心（Keeling Center） 德克萨斯州巴斯特罗夫市的安德森癌症中心拥有开会的设施，专业知识，经验和基础设施 SPFBRR的当前和未来需求。使用此赠款申请，我们建议搬迁SPFBRR 到Keeling Center，通过重新衍生婴儿的狒狒，加速SPF菌落的生长 常规（非SPF）繁殖殖民地，并通过研究计划维持和扩展合作伙伴关系 这些宝贵的SPF狒狒资源。在本申请中要求的支持期间 SPFBRR将扩大以创建一个自我维持的行为健康和 遗传上健壮的繁殖动物。这将消除对传统（非SPF）婴儿的需求 大坝，这将大大降低将晚期感染剂引入SPFBRR的风险。 该P40应用程序的总体目标是将SPFBRR搬迁到龙骨中心，以便这 可以保留必不可少的国家研究资源；向科学家提供教育和培训 与狒狒一起工作的兽医，殖民地经理和动物护理人员；进行应用研究 改善资源；并且支持需要设施和专业知识的研究人员的研究项目 涉及SPF狒狒。我们还将对主要组织相容性复合物进行mRNA测序 （MHC）I类等位基因SPFBRR的所有基础繁殖狒狒；而且，我们将表现完整 基因组和谱系分析资源中26只动物的基因组测序（WGS）。此信息 将进一步完善并为SPFBRR提供的资源增加价值。最初设计的设施 黑猩猩将被重新使用以满足SPFBRR和Keeling的其他研究设施的需求 中心将提供与SPF狒狒进行研究的空间。