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Screening for small molecule inhibitors of B. burgdorferi HtrA

Screening for small molecule inhibitors of B. burgdorferi HtrA
伯氏疏螺旋体HtrA小分子抑制剂的筛选
批准号：
10919702
负责人：
Alexey Zakharov
金额：
$24.08万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10919702
关键词：
Antibiotic TherapyAntibioticsAreaAwarenessBacteriaBiological AssayBorreliaBorrelia burgdorferiCaseinsCellsCharacteristicsChronicDevelopmentDiseaseEnzymesEtiologyFluorescence Resonance Energy TransferGenetic TranscriptionGrowthHelicobacter pyloriHigh temperature of physical objectHomologous GeneImmune responseImpairmentLabelLiteratureLyme DiseaseMolecular ChaperonesOrder SpirochaetalesPathogenesisPathogenicityPatientsPeptidesPost Treatment Lyme Disease SyndromePrevalenceProtein FamilyProteinsQuality ControlRecombinantsReportingSeriesSerine ProteaseSpecificitySurfaceTestingTherapeutic EffectTick-Borne DiseasesTissuesValidationVirulenceantimicrobialassay developmentexperienceextracellularfluorophorehigh throughput screeninginhibitorperiplasmredshiftscreeningsmall moleculesmall molecule inhibitortoolvirtual screening

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英文摘要
During the reporting period, the collaborative team began initial assay development during recombinant BbHtrA protein, and enzyme characterization is being completed with a casein derivative labeled with a red-shifted fluorophore. Validation screens with BbHtrA and the casein substrate have demonstrated assay robustness to enable larger-scale screening. Further characterization of enzyme specificity is being conducted using a FRET peptide screening approach in order to move towards a characterized FRET substrate necessary for screening for inhibitors.
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Screening for small molecule inhibitors of B. burgdorferi HtrA
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