Screening for small molecule inhibitors of B. burgdorferi HtrA

在报告所述期间，合作团队在重组BbHtrA蛋白期间开始了初步的分析开发，并且正在用红移荧光团标记的酪蛋白衍生物完成酶表征。BbHtrA和酪蛋白底物的验证筛选已经证明了分析稳健性，可以进行更大规模的筛选。目前正在使用FRET肽筛选方法进一步表征酶的特异性，以便获得筛选抑制剂所必需的表征FRET底物。

During the reporting period, the collaborative team began initial assay development during recombinant BbHtrA protein, and enzyme characterization is being completed with a casein derivative labeled with a red-shifted fluorophore. Validation screens with BbHtrA and the casein substrate have demonstrated assay robustness to enable larger-scale screening. Further characterization of enzyme specificity is being conducted using a FRET peptide screening approach in order to move towards a characterized FRET substrate necessary for screening for inhibitors.