Generating new mouse models

生成新的鼠标模型

• 批准号: 10908197
• 负责人: Karl Eric Pfeifer
• 金额: $ 91.98万
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10908197
• 关键词: Chemotherapy-Oncologic Procedure; ES Cell Line; Electroporation; Embryo; Enhancers; Epigenetic Process; Fertility; Gene Expression Regulation; Genome Stability; Goals; Hematopoiesis; Immune system; Implant; Injections; Intramural Research Program; Modeling; Mus; Mutant Strains Mice; Mutate; National Institute of Child Health and Human Development; Operative Surgical Procedures; Pathway interactions; Procedures; Protocols documentation; RNA metabolism; Research; Research Personnel; Research Support; Scientist; Signal Pathway; T-Cell Development; Thymus Gland; United States National Institutes of Health; Work; anti-cancer; blastocyst; design; embryonic stem cell; immune function; mouse model; novel; stem cell biology; zygote

During the last year our research core has worked with seven NIH research units to generate novel embryonic stem cell lines and new mouse models. This research included establishment of models to study signal pathway interactions in the immune system, T cell development, hematopoiesis, stem cell biology, epigenetic inheritance in the early embryo, mechanisms regulating genomic stability, RNA metabolism, and gene regulation and enhancer function. We also performed specialized surgeries including thymus implants to support research on immune function. Finally we established mouse models to evaluate the effects of anti-cancer chemotherapies given to prepubescent mice on their long term fertility.

在过去的一年里，我们的研究核心与美国国立卫生研究院的七个研究单位合作，创造了新的胚胎干细胞系和新的小鼠模型。这项研究包括建立模型以研究免疫系统、T细胞发育、造血、干细胞生物学、早期胚胎的表观遗传、调节基因组稳定性的机制、RNA代谢以及基因调节和增强子功能的信号通路相互作用。我们还进行了包括胸腺植入在内的专门手术，以支持免疫功能的研究。最后，我们建立了小鼠模型来评估给予青春期前小鼠的抗癌化疗对它们长期生育能力的影响。