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Neuroanalytical Core

神经分析核心

基本信息

批准号：
10626086
负责人：
MICHAEL L HEIEN
金额：
$ 23.49万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-15 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10626086
关键词：
Anesthesia procedures Animals Behavioral Biological Biosensor Brain Brain region Cells Chemicals Communities Corpus striatum structure Coupled Data Analyses Detection Dialysis procedure Diffusion Dimensions Disease Dopamine Drug Addiction Electrodes Exhibits Extracellular Space Functional disorder Funding Genetic Techniques Heterogeneity Interneurons Investigation Learning Link Measurement Measures Memory Mental Depression Mental disorders Methodology Methods Microdialysis Microelectrodes Molecular Monitor Motivation National Institute of Drug Abuse Neuronal Plasticity Neurons Neurosciences Neurotransmitters Pacemakers Parkinson Disease Pattern Periodicity Phase Physiological Play Preparation Reaction Time Research Resolution Role Sampling Scanning Schizophrenia Serotonergic System Serotonin Services Signal Transduction Site Slice Stimulus Synapses Synaptic plasticity System Techniques Time Tissues Work addiction carbon fiber design dopamine transporter dopaminergic neuron emotional behavior extracellular flexibility in vivo instrument interest kinetic model nervous system disorder neurochemistry neurotransmission pain signal pharmacologic phase change protein function receptor sensor spatiotemporal

项目摘要

Summary – Neuroanalytial Core The Neuroanalytical Core develops and applies methods to measure electroactive neurotransmitters in vivo. The core will develop and then use electrochemical methods to measure the absolute concentrations of easily oxidized neurotransmitters in vivo – something not possible with other methodology. Fast changes in the extracellular concentration of neurotransmitters can arise from phasic neuronal firing. For this reason, chemical sensors should be able to operate on a wide range of time scales. An ideal sensor for the detection of neurotransmitters has high sensitivity, can distinguish between compounds, and has a fast response time. Electrochemical approaches offer a way to accomplish this for easily oxidized neurotransmitters by using an electrode next to sites where the neurotransmitter is released. The core can monitor rapid, phasic changes using FSCV or slow, tonic changes using FSCAV. Making simultaneous measurements of dopamine and serotonin on multiple timescales has the potential to further understanding of the interactions between the two systems. It is important to note that in vivo experimentation will require informed selection of the brain regions of interest. This instrument allows for the investigation of the interdependence of the dopaminergic and serotonergic systems and the role they play in learning and disease states. Excitingly, this core has been designed to allow additional methods to be incorporated. This core can be used to further behavioral neuroscience and our current understanding of the links between neurochemical systems. We couple the methodologies to neuroscience to ensure that we will continue to advance the impact of the NIDA research community.

摘要 – 神经分析核心神经分析核心开发并应用测量体内电活性神经递质的方法。该核心将开发出来，然后使用电化学方法轻松测量绝对浓度体内氧化的神经递质——这是其他方法不可能实现的。快速变化神经递质的细胞外浓度可以由阶段性神经元放电引起。为此，化学传感器应该能够在很宽的时间范围内运行。用于检测的理想传感器神经递质灵敏度高，能够区分化合物，并且响应时间快。电化学方法提供了一种通过使用易于氧化的神经递质来实现这一目标的方法。电极靠近释放神经递质的部位。核心可以使用以下方式监控快速的阶段性变化 FSCAV 或使用 FSCAV 进行缓慢、强直的变化。同时测量多巴胺和血清素在多个时间尺度上进行研究有可能进一步了解两个系统之间的相互作用。它值得注意的是，体内实验需要明智地选择感兴趣的大脑区域。该仪器可以研究多巴胺能和血清素能的相互依赖性系统及其在学习和疾病状态中发挥的作用。令人兴奋的是，该核心的设计允许纳入其他方法。该核心可用于进一步发展行为神经科学和我们当前的了解神经化学系统之间的联系。我们将方法论与神经科学结合起来确保我们将继续提升 NIDA 研究界的影响力。