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Neuroanalytical Core

神经分析核心

基本信息

批准号：
10469428
负责人：
MICHAEL L HEIEN
金额：
$ 23.49万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-15 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10469428
关键词：
Animals Behavioral Biological Biosensor Brain Brain region Cells Chemicals Communities Corpus striatum structure Coupled Data Detection Dialysis procedure Diffusion Dimensions Disease Dopamine Drug Addiction Electrodes Ensure Exhibits Extracellular Space Functional disorder Funding Genetic Techniques Heterogeneity Interneurons Investigation Learning Link Measurement Measures Memory Mental Depression Mental disorders Methodology Methods Microdialysis Microelectrodes Molecular Monitor Motivation National Institute of Drug Abuse Neuronal Plasticity Neurons Neurosciences Neurotransmitters Oxides Pacemakers Parkinson Disease Pattern Periodicity Pharmacology Phase Physiological Play Preparation Reaction Time Research Resolution Role Sampling Scanning Schizophrenia Serotonergic System Serotonin Services Signal Transduction Site Slice Stimulus Synapses Synaptic plasticity System Techniques Time Tissues Work addiction base carbon fiber design dopamine transporter dopaminergic neuron emotional behavior extracellular flexibility in vivo instrument interest kinetic model nervous system disorder neurochemistry neurotransmission pain signal phase change protein function receptor sensor spatiotemporal

项目摘要

Summary – Neuroanalytial Core The Neuroanalytical Core develops and applies methods to measure electroactive neurotransmitters in vivo. The core will develop and then use electrochemical methods to measure the absolute concentrations of easily oxidized neurotransmitters in vivo – something not possible with other methodology. Fast changes in the extracellular concentration of neurotransmitters can arise from phasic neuronal firing. For this reason, chemical sensors should be able to operate on a wide range of time scales. An ideal sensor for the detection of neurotransmitters has high sensitivity, can distinguish between compounds, and has a fast response time. Electrochemical approaches offer a way to accomplish this for easily oxidized neurotransmitters by using an electrode next to sites where the neurotransmitter is released. The core can monitor rapid, phasic changes using FSCV or slow, tonic changes using FSCAV. Making simultaneous measurements of dopamine and serotonin on multiple timescales has the potential to further understanding of the interactions between the two systems. It is important to note that in vivo experimentation will require informed selection of the brain regions of interest. This instrument allows for the investigation of the interdependence of the dopaminergic and serotonergic systems and the role they play in learning and disease states. Excitingly, this core has been designed to allow additional methods to be incorporated. This core can be used to further behavioral neuroscience and our current understanding of the links between neurochemical systems. We couple the methodologies to neuroscience to ensure that we will continue to advance the impact of the NIDA research community.

摘要-神经分析核心神经分析核心开发和应用方法来测量体内电活性神经递质。该核心将制定，然后使用电化学方法来测量的绝对浓度很容易体内氧化的神经递质-这是其他方法不可能的。的快速改变神经递质的细胞外浓度可由阶段性神经元放电引起。因此，化学传感器应该能够在宽范围的时间尺度上操作。检测的理想传感器神经递质具有高灵敏度，可以区分化合物，并具有快速的响应时间。电化学方法提供了一种方法，通过使用电化学方法，电极旁边的网站，其中神经递质的释放。核心可以监测快速，阶段性的变化， FSCV或使用FSCAV的缓慢强直性变化。同时测量多巴胺和血清素在多个时间尺度上的研究有可能进一步了解这两个系统之间的相互作用。它重要的是要注意，体内实验将需要对感兴趣的大脑区域进行明智的选择。该仪器允许研究多巴胺能和多巴胺能的相互依赖性。系统及其在学习和疾病状态中的作用。令人兴奋的是，这个核心的设计允许将采用其他方法。这个核心可以用于进一步的行为神经科学和我们目前的了解神经化学系统之间的联系。我们将方法论与神经科学结合起来，确保我们将继续推进NIDA研究界的影响。