Optoretinography: All-optical measures of functional activity in the human retina

项目概要/摘要 视光视网膜图（ORG）作为正常眼视锥细胞功能的探针，在评估 疾病状态的锥在疾病中，并在治疗干预的评价。实现其 承诺，ORG的细胞和分子基础需要建立，并标准化方法， 在临床环境中的应用。这一补充将追求的研究机会，已经打开了 在目前的生物工程研究伙伴关系下，但以前没有预见到。其中包括 在啮齿动物视网膜光描记术中增加自适应光学器件以提高信号保真度和分辨率， 斯坦福大学。其次，在加州大学戴维斯分校和华盛顿大学之间的合作努力中，一个关键的差异是 发现以前在所有现存的措施色素漂白;这一补充将旨在解决这一问题 提出并阐明圆锥体ORG的机制基础。最后，患者友好型的快速发展 在UW的ORG变体加速了遗传性视网膜疾病患者的招募， 创造了一个机会，标准化ORG协议，研究基因型-表型相关性，在一个 临床设置。来自补编的支持将提供一个途径来探索这三个意想不到的， 令人兴奋的和关键的领域，从而提供协同作用的临床，技术和机械方面的 项目

Project Summary/Abstract The optoretinogram (ORG) holds great promise as a probe of cone function in normal eyes, in the assessment of disease states of cones in disease, and in the evaluation of therapeutic interventions. To achieve its promise, the cellular and molecular basis of the ORG needs to be established, and methods standardized for application in the clinical setting. This supplement will pursue research opportunities that have opened up under the current Bioengineering research partnership, but were previously unforeseen. These include the addition of adaptive optics to rodent optoretinography in order to improve signal fidelity and resolution at Stanford University. Second, in a collaborative effort between UC Davis and UW, a key discrepancy was discovered previously in all extant measures of pigment bleaching; this supplement will aim to resolve this issue and elucidate the mechanistic basis of the cone ORG. Lastly, the rapid development of a patient-friendly variant of the ORG at UW has accelerated the recruitment of patients with inherited retinal disease, and created an opportunity to standardize the ORG protocols for studying genotype-phenotype correlations in a clinical setting. Support from the supplement will provide an avenue to explore these three unanticipated, exciting and critical areas, thus providing synergy to the clinical, technological and mechanistic facets of the project.