Optoretinography: All-optical measures of functional activity in the human retina

项目摘要/摘要 在评估中，视网膜仪(ORG)作为正常眼的视锥功能的探测仪前景看好。 在疾病中的视锥细胞的疾病状态，以及在治疗干预措施的评估中。要实现ITS Promise，ORG的细胞和分子基础需要建立，方法需要标准化 在临床环境中的应用。本副刊将寻求已开启的研究机会。 在目前的生物工程研究伙伴关系下，但以前没有预见到。其中包括 为了提高信号保真度和分辨率，在啮齿动物视网膜成像中增加了自适应光学系统 斯坦福大学。其次，在加州大学戴维斯分校和威斯康星州大学的合作努力中，一个关键的差异是 以前在所有现有的颜料漂白方法中发现；这一补充将旨在解决这一问题 提出并阐明了锥形ORG的力学基础。最后，快速发展了一种方便患者的 华盛顿大学的ORG变种加速了遗传性视网膜疾病患者的招募，以及 创造了标准化ORG协议的机会，用于研究基因-表型相关性 临床环境。来自副刊的支持将提供一条途径来探索这三个意想不到的问题， 令人兴奋和关键的领域，从而为临床、技术和机制方面提供协同作用 项目。

Project Summary/Abstract The optoretinogram (ORG) holds great promise as a probe of cone function in normal eyes, in the assessment of disease states of cones in disease, and in the evaluation of therapeutic interventions. To achieve its promise, the cellular and molecular basis of the ORG needs to be established, and methods standardized for application in the clinical setting. This supplement will pursue research opportunities that have opened up under the current Bioengineering research partnership, but were previously unforeseen. These include the addition of adaptive optics to rodent optoretinography in order to improve signal fidelity and resolution at Stanford University. Second, in a collaborative effort between UC Davis and UW, a key discrepancy was discovered previously in all extant measures of pigment bleaching; this supplement will aim to resolve this issue and elucidate the mechanistic basis of the cone ORG. Lastly, the rapid development of a patient-friendly variant of the ORG at UW has accelerated the recruitment of patients with inherited retinal disease, and created an opportunity to standardize the ORG protocols for studying genotype-phenotype correlations in a clinical setting. Support from the supplement will provide an avenue to explore these three unanticipated, exciting and critical areas, thus providing synergy to the clinical, technological and mechanistic facets of the project.