ORGANIZATION OF LH RECEPTORS ON HORMONE RESPONSIVE CELLS

Roess博士的研究涉及黄体生成的研究 激素/绒毛膜促性腺激素(LH/CG)受体组织及其如何 组织调节受体的功能。她的长期目标是做得更好 了解黄体生成素/卵泡刺激素受体结构在生育调节中的作用 促性腺激素如何调节黄体生成素/卵泡刺激素受体功能事件 包括G蛋白相互作用、受体内化和cAMP 激活。在颁奖期间，她将检查促性腺激素 结合导致功能性黄体生成素受体复合体的大小变化以及 受体-受体在膜上聚集的生物物理法 技巧。这些研究将使用人类胚胎肾脏细胞 不同C端截短对黄体生成素受体表达的影响 CAMP激活和受体内化。的影响 脱糖hCG与天然hCG结合对黄体生成素/卵泡刺激素受体结合的影响 膜内将在MA-10细胞上进行评估，其中结合 人绒毛膜促性腺激素去糖化到黄体生成素/卵泡刺激素受体减缓受体的调节 和内部化。然后是非受体蛋白的相互作用 包括G蛋白亚基和MHC-I类抗原与黄体生成素/卵泡刺激素受体 将通过一种光邻近标记技术进行探索，该技术允许 已知基因物理邻近蛋白的鉴定和分离 膜部位。

Dr. Roess' research has involved the study of luteinizing hormone/chorionic gonadotropin (LH/CG) receptor organization and how this organization modulates receptor function. Her long-term goal is to better understand the role of LH/CG receptor structure in regulation of fertility and how gonadotropins may modulate LH/CG receptor functional events including G-protein interactions, receptor internalization and cAMP activation. During the award period she will examine whether gonadotropin binding causes size changes in functional LH receptor complexes as well as receptor-receptor aggregation on the membrane using biophysical techniques. These studies will use human embryonic kidney cells expressing LH/CG receptors with various C-terminal truncations that affect cAMP activation and receptor internalization. The effects of deglycosylated hCG and native hCG binding on LH/CG receptor association within the membrane will be evaluated on MA-10 cells where binding of deglycosylated hCG to the LH/CG receptor slows receptor down regulation and internalization. Then the association of non-receptor proteins including G-protein subunits and MHC class I antigen with LH/CG receptors will be explored by a photoproximity labeling technique that permits identification and isolation of proteins physically proximal to a known membrane site.