WORKSHOP--SITE-SPECIFIC RECOMBINATION & TRANSPORTATION

请求为特定地点重组研讨会提供部分支持 和换位将于1990年9月12日至16日在海军陆战队举行 马萨诸塞州伍兹霍尔的生物实验室。 这个研讨会的目的是召集一批精选的 一群积极致力于 部位特异性生化机制研究的前沿 重组和转位。这一组相互关联的反应 在许多非常重要的生物过程的核心： 整合逆转录病毒，包括那些对艾滋病和 某些形式的癌症，产生抗体多样性，传播 抗生素耐药性基因，细菌染色体重排，以及 真核生物内含子的移动性。 本次研讨会的口头陈述将强调机械性 这些重组反应的各个方面。一次专门的领导人会议 在这一领域的工作应该既及时又富有成效。已经有了很快的 在许多不同系统中的最新进展。此外，它正在成为 越来越明显的是，真核生物中发生的反应 与细菌中发现的那些密切相关：艾滋病的整合 病毒和其他逆转录病毒在机制上与 细菌转座子、位点特异性免疫球蛋白的整合 重排与原核位点特异性密切相关 重组反应等。在促进交流见解和 来自不同系统的信息，研讨会应该大大增强我们的 对所有这些反应有基本的了解。此外，在 艾滋病病毒和其他逆转录病毒的特殊情况下，它应该进一步 将重组反应作为靶向治疗的目标 干预。 演讲者名单上包括应用生物化学、 遗传和拓扑分析，包括几位推动 分析重组首次进入酶的领域 运动学。主题包括逆转录病毒整合，病毒的反应 整合酶和分解酶家族、Holliday连接、免疫球蛋白 重排、原核转座子与果蝇P 元素。 我们还鼓励来自科学家们的海报展示 还不能进行机械分析的系统。这应该是 既有助于扩大会议的生物学基础，又有助于鼓励 那些在相对不发达的系统中工作的人承担更多的 机械论的方法。 会议将限制在100-135人参加。这个号码是 足以包括所有正在工作的主要调查人员 在这一领域有成效，以及合理的博士后样本 研究员和偶尔出类拔萃的研究生。不过，这个 数字足够小，以便在所有 参与者仍然是可能的。

Partial support is requested for a Workshop on Site-specific Recombination and Transposition to be held on September 12-16, 1990, at the Marine Biological Laboratory in Woods Hole, Massachusetts. The purpose of this workshop is to assemble for discussions a select but representative group of scientists who are actively working at the forefront of research on the biochemical mechanisms for site-specific recombination and transposition. This interrelated group of reactions lie at the heart of a number of very important biological processes: integration of retroviruses including those responsible for AIDS and certain forms of cancer, generation of antibody diversity, transmission of antibiotic resistance genes, rearrangement of bacterial chromosomes, and mobility of eukaryotic introns. The oral presentations at this Workshop will emphasize the mechanistic aspects of these recombination reactions. A specialized meeting for leaders in this field should be both timely and productive. There has been rapid recent progress in many different systems. Furthermore, it is becoming increasingly apparent that reactions occurring in eukaryotic organisms are intimately related to those identified in bacteria: integration of AIDS virus and other retroviruses is mechanistically closely related to integration of bacterial transposons, site specific immunoglobulin rearrangements are closely related to prokaryotic site specific recombination reactions, etc. In promoting the exchange of insights and information from different systems, the Workshop should greatly enhance our basic understanding of all of these reactions. In addition, in the particular case of AIDS virus and other retroviruses, it should further the goal of using the recombination reaction as a target therapeutic intervention. The roster of speakers includes scientists who have applied biochemical, genetic and topological analysis including several who have pushed the analysis of recombination for the first time into the realm of enzyme kinetics. Topics will include retroviral integration, reactions of the integrase and resolvase families, Holliday junctions, immunoglobulin rearrangements, prokaryotic transposable elements, and Drosophila P elements. We are also encouraging poster presentations from scientists working in systems which are not yet amenable to mechanistic analysis. This should serve both to broaden the biological base of the meeting and to encourage those working in relatively undeveloped systems to undertake a more mechanistic approach. The meeting will be limited to 100-135 participants. This number is sufficient to include all of the principal investigators who are working productively in this area as well as a reasonable sampling of postdoctoral fellows and an occasional outstanding graduate student. However, this number is small enough that efficient interchange amongst all of the participants is still possible.