Human Embryonic Stem Cell Core

人胚胎干细胞核心

• 批准号: 7901349
• 负责人: Zoran Galic
• 金额: $ 36.02万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7901349
• 关键词: Architecture; Cell Culture Techniques; Cell Line; Cell Maintenance; Cell Survival; Cells; Characteristics; Chromosome abnormality; Core Facility; Ensure; Environment; Epigenetic Process; Genetic; Genomics; Goals; Growth; Growth Factor; Lead; Methods; Monitor; Population; Production; Protocols documentation; Publishing; Quality Control; Reagent; Research; Research Personnel; Services; Stem Cell Research; Stem cells; Surface; Undifferentiated; United States National Institutes of Health; Work; cell growth; design; human embryonic stem cell; human embryonic stem cell line; improved; investigator training; new technology; novel; programs; self-renewal; vector

Human embryonic stem cell (hESC) research depends upon the ability of these cells to remain pluripotent and genetically stable in culture as a self-renewing population. This stability and functionality must also be maintained after directed differentiation into multiple cellular lineages. Current culture conditions for hESCs are sub-optimal and this can lead to acquisition of chromosome abnormalities, sporadic differentiation, genomic alterations and decreased cell viability. Before hESCs will be useful clinically, standard protocols must be developed to ensure accurate and repeatable methods for hESC maintenance, culture and differentiation. Although most of the NIH approved lines share common characteristics, a wide range of culture conditions have been published, which makes comparisons of different methods difficult. The hESC Core Facility (Core B) is developing standardized hESC culture methods with the goal of assisting researchers in this program project with hESC culture techniques. The core will provide expertise in growing, splitting, monitoring and determining valid protocols for hESC culture methods. In addition, the core will develop crucial reagents for each of the projects, as detailed in Projects 1-3. Core B will provide a means for project integration and comparison, and also train investigators as new technology develops. Core B will provide novel biosynthetic culture surfaces to optimize hESC growth in both undifferentiated and differentiated states. Thus, Core B will provide both service and experimental activities for the benefit of the program project. The Specific Aims of the Stem Cell Core are: Specific Aim 1. To culture, bank and distribute hESCs for the program project Specific Aim 2. Monitor differences over prolonged culture conditions with multiple hESC cell lines Specific Aim 3. Optimize growth conditions with multiple hESC lines as needed for each project Specific Aim 4. Provide hESCs with stable vector integration and lysates as needed per project Specific Aim 5. Develop new directions in synthetic materials and architectures Specific Aim 6. Integrate projects and facilitate transfer of information between each project

人类胚胎干细胞（hESC）的研究依赖于这些细胞保持多能性的能力 在文化中作为一个自我更新的群体遗传稳定。这种稳定性和功能性也必须 在定向分化成多个细胞谱系后维持。hESC的当前培养条件 是次优的，这可能导致染色体异常的获得，零星的分化， 基因组改变和细胞活力降低。在胚胎干细胞应用于临床之前， 必须开发，以确保准确和可重复的方法hESC的维护，培养和 分化虽然大多数NIH批准的细胞系具有共同的特征，但广泛的 已经公布了培养条件，这使得不同方法的比较变得困难。 hESC核心设施（核心B）正在开发标准化hESC培养方法，目标是协助 研究人员在这个项目的项目与hESC培养技术。核心将提供以下方面的专门知识 生长、分裂、监测和确定hESC培养方法的有效方案。另外核心 将为每个项目开发关键试剂，详见项目1-3。核心B将提供一种方法 用于项目整合和比较，并随着新技术的发展培训调查人员。核心B将 提供了新的生物合成培养表面，以优化hESC在未分化和未分化细胞中的生长， 不同的国家。因此，核心B将提供服务和实验活动， 程序项目。 干细胞核心的具体目标是： 具体目标1.培养、储存和分配hESC用于项目 具体目标2。监测多种hESC细胞系在长期培养条件下的差异 具体目标3。根据每个项目的需要，使用多个hESC系优化生长条件 具体目标4。根据项目需要，为hESC提供稳定的载体整合和裂解物 具体目标5.开发合成材料和建筑的新方向 具体目标6.整合项目并促进各项目之间的信息传递