Human Embryonic Stem Cell Core

人胚胎干细胞核心

• 批准号: 8123452
• 负责人: Zoran Galic
• 金额: $ 35.55万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8123452
• 关键词: Architecture; Cell Culture Techniques; Cell Line; Cell Maintenance; Cell Survival; Cells; Characteristics; Chromosome abnormality; Core Facility; Ensure; Environment; Epigenetic Process; Genetic; Genomics; Goals; Growth; Growth Factor; Lead; Methods; Monitor; Population; Production; Protocols documentation; Publishing; Quality Control; Reagent; Research; Research Personnel; Services; Stem Cell Research; Stem cells; Surface; Undifferentiated; United States National Institutes of Health; Work; cell growth; design; human embryonic stem cell; human embryonic stem cell line; improved; investigator training; new technology; novel; programs; self-renewal; vector

Human embryonic stem cell (hESC) research depends upon the ability of these cells to remain pluripotent and genetically stable in culture as a self-renewing population. This stability and functionality must also be maintained after directed differentiation into multiple cellular lineages. Current culture conditions for hESCs are sub-optimal and this can lead to acquisition of chromosome abnormalities, sporadic differentiation, genomic alterations and decreased cell viability. Before hESCs will be useful clinically, standard protocols must be developed to ensure accurate and repeatable methods for hESC maintenance, culture and differentiation. Although most of the NIH approved lines share common characteristics, a wide range of culture conditions have been published, which makes comparisons of different methods difficult. The hESC Core Facility (Core B) is developing standardized hESC culture methods with the goal of assisting researchers in this program project with hESC culture techniques. The core will provide expertise in growing, splitting, monitoring and determining valid protocols for hESC culture methods. In addition, the core will develop crucial reagents for each of the projects, as detailed in Projects 1-3. Core B will provide a means for project integration and comparison, and also train investigators as new technology develops. Core B will provide novel biosynthetic culture surfaces to optimize hESC growth in both undifferentiated and differentiated states. Thus, Core B will provide both service and experimental activities for the benefit of the program project. The Specific Aims of the Stem Cell Core are: Specific Aim 1. To culture, bank and distribute hESCs for the program project Specific Aim 2. Monitor differences over prolonged culture conditions with multiple hESC cell lines Specific Aim 3. Optimize growth conditions with multiple hESC lines as needed for each project Specific Aim 4. Provide hESCs with stable vector integration and lysates as needed per project Specific Aim 5. Develop new directions in synthetic materials and architectures Specific Aim 6. Integrate projects and facilitate transfer of information between each project

人类胚胎干细胞 (hESC) 研究取决于这些细胞保持多能性的能力 作为一个自我更新的群体，在文化中遗传稳定。这种稳定性和功能性也必须 定向分化为多种细胞谱系后维持。目前 hESC 的培养条件 是次优的，这可能导致染色体异常、零星分化、 基因组改变和细胞活力降低。在 hESC 应用于临床之前，需要制定标准方案 必须开发确保 hESC 维持、培养和检测方法准确且可重复的方法 差异化。尽管大多数 NIH 批准的细胞系具有共同的特征，但范围广泛 培养条件已公布，这使得不同方法之间的比较变得困难。 hESC 核心设施（核心 B）正在开发标准化 hESC 培养方法，旨在协助 该项目的研究人员采用 hESC 培养技术。该核心将提供以下方面的专业知识 生长、分裂、监测和确定 hESC 培养方法的有效方案。此外，核心 将为每个项目开发关键试剂，详见项目 1-3。核心B将提供一种手段 用于项目整合和比较，并随着新技术的发展培训研究人员。核心B将 提供新颖的生物合成培养表面，以优化未分化和未分化 hESC 的生长 差异化的状态。因此，核心 B 将提供服务和实验活动，以造福于 计划项目。 干细胞核心的具体目标是： 具体目标 1. 为该计划项目培养、储存和分发 hESC 具体目标 2. 监测多种 hESC 细胞系在长期培养条件下的差异 具体目标 3. 根据每个项目的需要优化多个 hESC 系的生长条件 具体目标 4. 根据项目需要为 hESC 提供稳定的载体整合和裂解物 具体目标 5. 开发合成材料和建筑的新方向 具体目标 6. 整合项目并促进每个项目之间的信息传输