Human Embryonic Stem Cell Core

人类胚胎干细胞(HESC)的研究依赖于这些细胞保持多能性的能力。 作为一个自我更新的种群，在文化中基因稳定。这种稳定性和功能性还必须 定向分化为多个细胞系后维持。人类胚胎干细胞的培养现状 都是次优的，这可能导致获得染色体异常，零星分化， 基因组改变和细胞存活率下降。在人类胚胎干细胞将用于临床之前，标准方案 必须制定确保hESC维护、培养和管理的准确和可重复的方法 差异化。尽管NIH批准的大多数线路都有共同的特征，但广泛的 培养条件已经公布，这使得不同方法的比较变得困难。 HESC核心设施(核心B)正在开发标准化的hESC培养方法，目的是帮助 该项目的研究人员使用了hESC培养技术。该核心将在以下方面提供专业知识 生长、分离、监测和确定hESC培养方法的有效方案。此外，核心 将为每个项目开发关键试剂，如项目1-3所述。核心B将提供一种手段 用于项目整合和比较，并随着新技术的发展对调查人员进行培训。核心B将 提供新的生物合成培养表面以优化hESC在未分化和 差异化状态。因此，核心B将提供服务和实验活动，以造福于 计划项目。 干细胞核心的具体目标是： 具体目标1.为计划项目培养、储存和分发人类胚胎干细胞 特定目标2.监测多个hESC细胞系在延长培养条件下的差异 具体目标3.根据每个项目的需要，使用多个hESC品系优化生长条件 具体目标4.根据项目需要为人类胚胎干细胞提供稳定的载体整合和裂解产物 具体目标5.发展合成材料和建筑的新方向 具体目标6.整合项目并促进每个项目之间的信息转移

Human embryonic stem cell (hESC) research depends upon the ability of these cells to remain pluripotent and genetically stable in culture as a self-renewing population. This stability and functionality must also be maintained after directed differentiation into multiple cellular lineages. Current culture conditions for hESCs are sub-optimal and this can lead to acquisition of chromosome abnormalities, sporadic differentiation, genomic alterations and decreased cell viability. Before hESCs will be useful clinically, standard protocols must be developed to ensure accurate and repeatable methods for hESC maintenance, culture and differentiation. Although most of the NIH approved lines share common characteristics, a wide range of culture conditions have been published, which makes comparisons of different methods difficult. The hESC Core Facility (Core B) is developing standardized hESC culture methods with the goal of assisting researchers in this program project with hESC culture techniques. The core will provide expertise in growing, splitting, monitoring and determining valid protocols for hESC culture methods. In addition, the core will develop crucial reagents for each of the projects, as detailed in Projects 1-3. Core B will provide a means for project integration and comparison, and also train investigators as new technology develops. Core B will provide novel biosynthetic culture surfaces to optimize hESC growth in both undifferentiated and differentiated states. Thus, Core B will provide both service and experimental activities for the benefit of the program project. The Specific Aims of the Stem Cell Core are: Specific Aim 1. To culture, bank and distribute hESCs for the program project Specific Aim 2. Monitor differences over prolonged culture conditions with multiple hESC cell lines Specific Aim 3. Optimize growth conditions with multiple hESC lines as needed for each project Specific Aim 4. Provide hESCs with stable vector integration and lysates as needed per project Specific Aim 5. Develop new directions in synthetic materials and architectures Specific Aim 6. Integrate projects and facilitate transfer of information between each project