Core--Laboratory

核心B：该实验室核心将提供高质量的实验室技术，以支持所有四个项目 在本STI TM CRC申请中提出的包括：沙眼衣原体的微生物培养， 阴道毛滴虫、需氧和厌氧细菌及支原体. 细菌和T.迷走神经;真实的时间PCR定量T.迷走神经; C.沙眼 酸扩增法检测C.沙眼衣原体、淋病奈瑟菌和生殖支原体;以及 上皮细胞培养物。此外，实验室核心将提供中央标本处理， 分配资源，将协调向核心实验室和个人分配标本 研究实验室，并将协调运输和接收标本，并从合作 机构职能体系计算机数据库将用于跟踪样本的分布和存储位置， 残留标本、培养物和PCR产物。最后，实验室核心将进一步发展， 变性梯度凝胶电泳技术作为研究阴道生态系统的工具，包括 分析该方法的解释价值，提高测定通量， 计算机化系统来管理由该方法产生的复杂数据，以及该方法的应用。 研究过程中获得的连续阴道拭子样本的技术， 在几个CRC项目中进行。 所有这些活动对于实现海湾南部STI TM CRC的目标至关重要， 本提案的概述部分。

Core B: This laboratory core will provide high quality laboratory technology in support of all four projects proposed in this STI TM CRC application including: organism culture for Chlamydia trachomatis, Trichomonas vaginalis, aerobic and anaerobic bacteria and mycoplasmas; antibiotic susceptibility testing of bacteria and T. vaginalis; real time PCR quantification of T. vaginalis; genotyping of C. trachomatis; nucleic acid amplification assys for C. trachomatis, Neiserria gonorrhoeae and Mycoplasma genitalium; and epithelial cell cultures. Additionally the laboratory core will provide a central specimen processing and distribution resource that will coordinate distribution of specimens to the core laboratory and to the individual research laboratories and will coordinate shipping and receiving specimens to and from collaborating institutions. Computer data bases will be used to track specimen distribution and storage locations of remnant specimens, cultured organisms and PCR products. Finally the lab core will further development of denaturing gradient gel electrophoresis technology as a tool for the study of the vaginal ecosystem including analysis of the explanatory values of the approach, improving assay throughput, development of computerized systems to manage the complex data produced by this method and application of the technique to sequential vaginal swab specimens that will be obtained in the course of studies that will be carried out in several of the CRC projects. All of these activites are essential for meeting the objectives of the Gulf South STI TM CRC as stated in the Overview section of this proposal.