Core--Laboratory

核心B：这个实验室核心将为所有四个项目提供高质量的实验室技术 在这项STI TM CRC应用中提出的包括：沙眼衣原体的生物培养， 阴道毛滴虫需氧和厌氧细菌和支原体.抗生素敏感性试验 细菌和阴道毛滴虫；阴道毛滴虫的实时定量聚合酶链式反应；沙眼衣原体的基因分型；核酸 沙眼衣原体、淋球菌和生殖支原体的酸扩增系统；以及 上皮细胞培养。此外，实验室核心将提供中央样品处理和 将协调将标本分配给核心实验室和个人的分配资源 研究实验室，并将协调运送和从合作的样本接收 机构。将使用计算机数据库来跟踪标本的分发和储存位置 残留标本、培养生物和聚合酶链式反应产物。最后，实验室核心将进一步开发 变性梯度凝胶电泳技术作为研究阴道生态系统的工具，包括 分析该方法的解释价值，提高分析吞吐量，开发 管理由这种方法产生的复杂数据的计算机化系统和应用 对将在研究过程中获得的阴道拭子样本进行排序的技术 在几个《儿童权利公约》项目中开展了这项工作。 所有这些活动对于实现#年所述的海湾南部STI TM CRC的目标是至关重要的。 本建议书的概述部分。

Core B: This laboratory core will provide high quality laboratory technology in support of all four projects proposed in this STI TM CRC application including: organism culture for Chlamydia trachomatis, Trichomonas vaginalis, aerobic and anaerobic bacteria and mycoplasmas; antibiotic susceptibility testing of bacteria and T. vaginalis; real time PCR quantification of T. vaginalis; genotyping of C. trachomatis; nucleic acid amplification assys for C. trachomatis, Neiserria gonorrhoeae and Mycoplasma genitalium; and epithelial cell cultures. Additionally the laboratory core will provide a central specimen processing and distribution resource that will coordinate distribution of specimens to the core laboratory and to the individual research laboratories and will coordinate shipping and receiving specimens to and from collaborating institutions. Computer data bases will be used to track specimen distribution and storage locations of remnant specimens, cultured organisms and PCR products. Finally the lab core will further development of denaturing gradient gel electrophoresis technology as a tool for the study of the vaginal ecosystem including analysis of the explanatory values of the approach, improving assay throughput, development of computerized systems to manage the complex data produced by this method and application of the technique to sequential vaginal swab specimens that will be obtained in the course of studies that will be carried out in several of the CRC projects. All of these activites are essential for meeting the objectives of the Gulf South STI TM CRC as stated in the Overview section of this proposal.