The Role of Mycoplasma Genitalium in Infertility

生殖支原体在不孕症中的作用

• 批准号: 7979774
• 负责人: DAVID H. MARTIN
• 金额: $ 12.47万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7979774
• 关键词: Address; Antigens; Bacterial Adhesins; Biological Assay; Chronic; Cicatrix; Code; Complication; Data; Ectopic Pregnancy; Endometritis; Enrollment; Environment; Fallopian Tube Diseases; Genes; Genetic; Genital system; Genitourinary system; Healthcare; High Risk Woman; Human; Immune response; Immune system; Individual; Infection; Infertility; Lipoproteins; Local Microbicides; Louisiana; Mammalian Oviducts; Medical center; Membrane Proteins; Methods; Mutation; Mycoplasma genitalium; Organism; Pan Genus; Pelvic Inflammatory Disease; Population; Prevention; Proteins; Randomized; Recombinants; Reporting; Research; Risk; Role; Screening procedure; Serological; Sexual Partners; Specimen; Surface; Time; Universities; Urethritis; Woman; cohort; effective therapy; high risk; men; pathogen; prevent; research study; sample collection; treatment trial

Mycoplasma genitalium has been found to be associated with nongonococcal urethritis in men and there is some evidence to suggest that it may be a significant pathogen in women as well. It has been associated with mucopurulent cervictis in two studies. The major complication of bacterial STIs in women is infection of the upper genital tract resulting in pelvic inflammatory disease (PID) and fallopian tube scarring, which in turn cause ectopic pregnancy and/or infertility. There are a few reports suggesting that M. genitalium also causes these complications but these studies have not yielded consistent results. One serologic study suggests that the organism is associated with "tubal factor" infertility. If this should in fact turn out to be true then M. genitalium will be added to the list of significant bacterial STIs that should be prevented through effective treatment of symptomatic individuals and their sexual partners and by screening and treatment of asymptomatic high risk women and men. We will address this important issue in the first two Aims of this study as follows: 1. We will study a group of infertile women with and without fallopian tube disease drawn from a high STI risk population in New Orleans using our well established MG PCR assay and a specific serologic assay using recombinant M. genitalium lipoprotein antigens that we will develop. 2. We will study women at high risk of becoming infertile enrolled in a randomized infertility prevention treatment trial. Finally, in the third Aim we will study genetic mechanisms that this organism may use to escape the host immune system using specimens obtained from chronically infected chimpanzees and humans

已发现生殖支原体与男性非淋菌性尿道炎有关，并且 一些证据表明它也可能是女性的重要病原体。已经关联了 两项研究显示粘液脓性宫颈炎。女性细菌性性传播感染的主要并发症是感染 上生殖道导致盆腔炎 (PID) 和输卵管疤痕，进而 导致宫外孕和/或不孕。有一些报告表明生殖支原体也会导致 但这些研究并未得出一致的结果。一项血清学研究表明 该生物体与“输卵管因素”不孕症有关。如果事实证明这是真的，那么 M. 生殖器感染将被添加到重要的细菌性性传播感染清单中，应通过有效的措施来预防 对有症状的个体及其性伴侣进行治疗，并通过筛查和治疗 无症状的高危女性和男性。我们将在本文的前两个目标中解决这个重要问题 研究如下： 1. 我们将抽取一组患有和不患有输卵管疾病的不孕女性进行研究 使用我们完善的 MG PCR 检测和特定的方法从新奥尔良的高 STI 风险人群中进行检测 使用我们将开发的重组生殖支原体脂蛋白抗原进行血清学测定。 2.我们会学习 不孕症高风险女性参加了一项随机不孕症预防治疗试验。最后， 在第三个目标中，我们将研究该生物体可能用来逃避宿主免疫的遗传机制 使用从慢性感染的黑猩猩和人类身上获得的样本的系统