Core--Laboratory

核心--实验室

• 批准号: 6866143
• 负责人: DAVID H. MARTIN
• 金额: $ 33.58万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2009-07-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6866143
• 关键词: Chlamydia trachomatis; Mycoplasma; Neisseria gonorrhoeae; Trichomonas vaginalis; biomedical facility; cooperative study; data management; denaturing gradient gel electrophoresis; drug screening /evaluation; human tissue; method development; microorganism culture; microorganism resource /registry; polymerase chain reaction; sexually transmitted diseases; tissue /cell culture

This laboratory core will provide high quality laboratory technology in support of all four projects proposed in this STI TM CRC application including: organism culture for Chlamydia trachomatis, Trichomonas vaginalis, aerobic and anaerobic bacteria and mycoplasmas; antibiotic susceptibility testing of bacteria and T. vaginalis; real time PCR quantification of T. vaginalis; genotyping of C. trachomatis; nucleic acid amplification assys for C. trachomatis, Neiserria gonorrhoeae and Mycoplasma genitalium; and epithelial cell cultures. Additionally the laboratory core will provide a central specimen processing and distribution resource that will coordinate distribution of specimens to the core laboratory and to the individual research laboratories and will coordinate shipping and receiving specimens to and from collaborating institutions. Computer data bases will be used to track specimen distribution and storage locations of remnant specimens, cultured organisms and PCR products. Finally the lab core will further development of denaturing gradient gel electrophoresis technology as a tool for the study of the vaginal ecosystem including analysis of the explanatory values of the approach, improving assay throughput, development of computerized systems to manage the complex data produced by this method and application of the technique to sequential vaginal swab specimens that will be obtained in the course of studies that will be carried out in several of the CRC projects. All of these activites are essential for meeting the objectives of the Gulf South STI TM CRC as stated in the Overview section of this proposal.

该实验室核心将提供高质量的实验室技术，支持 STI TM CRC 申请中提出的所有四个项目，包括：沙眼衣原体、阴道毛滴虫、需氧和厌氧细菌以及支原体的生物体培养；细菌和阴道毛滴虫的抗生素敏感性测试；阴道毛滴虫的实时 PCR 定量；沙眼衣原体的基因分型；沙眼衣原体、淋病奈瑟菌和生殖支原体核酸扩增分析系统；和上皮细胞培养物。此外，核心实验室将提供中央标本处理和分发资源，协调标本到核心实验室和各个研究实验室的分发，并协调合作机构之间的标本运输和接收。计算机数据库将用于追踪样本分布以及剩余样本、培养生物和PCR产物的存储位置。最后，实验室核心将进一步开发变性梯度凝胶电泳技术，作为研究阴道生态系统的工具，包括分析该方法的解释值、提高检测通量、开发计算机化系统来管理该方法产生的复杂数据，以及将该技术应用于将在几个 CRC 项目进行的研究过程中获得的连续阴道拭子样本。 所有这些活动对于实现本提案概述部分所述的海湾南部 STI TM CRC 目标至关重要。