Genome-Wide Association Studies of Adiposity in Samoans

描述（由申请人提供）：“萨摩亚人肥胖症的全基因组关联研究”这项新申请的目标是确定成年萨摩亚人肥胖症和肥胖症相关表型的遗传变异。我们将使用全基因组关联方法，利用更新更强大的技术来识别与肥胖表型相关的单核苷酸多态性（SNP）变异。我们将利用萨摩亚农村地区2 500名成年人的人口样本进行这些研究，萨摩亚是一个正在经历营养转型的独立国家。我们将通过进一步分析三个不同的研究样本来复制我们对重要SNP的全基因组关联研究的结果：来自美属萨摩亚和萨摩亚早期研究的2,500名萨摩亚人的独立样本，以及来自克罗地亚的1,500名成年人和来自中国的1,000名成年人。然后，我们将确定重要基因的序列，并确定这三个样本中与肥胖相关的测序基因中的特定遗传变异。最后，我们将探讨基因与环境的相互作用，利用已确定的基因变异的测序区域和萨摩亚的生活方式和营养行为的实质性环境异质性。从这项研究中获得的知识将有助于萨摩亚人（包括美国人）肥胖干预措施的制定，并可能推广到其他太平洋岛民群体。公共卫生相关性：本申请的目的是使用全基因组关联方法确定成年萨摩亚人肥胖和肥胖相关表型的遗传变异。将使用一组约900，000个SNP进行基因分型，并将从居住在萨摩亚农村地区的2，500名成年萨摩亚人中收集肥胖表型。

DESCRIPTION (provided by applicant): "Genome-wide association studies of adiposity in Samoans" The goal of this new application is to define the genetic variation that underlies obesity and obesity-related phenotypes among adult Samoans. We will use genome-wide association methods to take advantage of newer more powerful techniques to identify single nucleotide polymorphism (SNP) variants that are associated with adiposity phenotypes. We will conduct these studies using a population sample of 2,500 adults from rural areas of Samoa, an independent nation experiencing the nutritional transition. We will replicate the findings from our genome-wide association studies of important SNPs by further analysis in three different study samples: an independent sample of 2,500 Samoans from earlier studies in American Samoa and Samoa, and 1,500 adults from Croatia and 1,000 adults from China. We will then determine the sequences of the important genes and determine the specific genetic variants in the sequenced genes that are associated with obesity in these three samples. Finally, we will explore gene-environment interactions, taking advantage of identified genetic variants in the sequenced regions and the substantial environmental heterogeneity in lifestyle and nutritional behaviors within Samoa. The knowledge gained from this research will aid the development of interventions on obesity amon Samoans including those in the US and may be generalizable to other Pacific Islander groups. PUBLIC HEALTH RELEVANCE: The goal of this application is to define the genetic variation that underlies obesity and obesity-related phenotypes among adult Samoans using genome-wide association methods. Genotyping will be performed using a panel of approximately 900,000 SNPs, and adiposity phenotypes will be collected from 2,500 adult Samoans residing in rural regions of Samoa.