Genome-Wide Association Studies of Adiposity in Samoans

描述（由申请人提供）：“萨摩亚人肥胖的全基因组关联研究”这项新申请的目标是定义成年萨摩亚人肥胖和肥胖相关表型的遗传变异。我们将使用全基因组关联方法来利用更新更强大的技术来识别与肥胖表型相关的单核苷酸多态性（SNP）变异。我们将利用萨摩亚农村地区2500名成年人的人口样本进行这些研究，萨摩亚是一个正在经历营养转型的独立国家。我们将通过在三个不同的研究样本中进一步分析，复制我们对重要snp的全基因组关联研究的发现：一个独立样本，包括来自早期在美属萨摩亚和萨摩亚进行研究的2500名萨摩亚人，以及来自克罗地亚的1500名成年人和来自中国的1000名成年人。然后，我们将确定重要基因的序列，并确定与这三个样本中肥胖相关的测序基因中的特定遗传变异。最后，我们将探索基因与环境的相互作用，利用已测序区域中已确定的遗传变异以及萨摩亚生活方式和营养行为的巨大环境异质性。从这项研究中获得的知识将有助于制定萨摩亚人（包括美国人）的肥胖干预措施，并可能推广到其他太平洋岛民群体。公共卫生相关性：本应用程序的目标是使用全基因组关联方法确定成年萨摩亚人肥胖和肥胖相关表型的遗传变异。将使用约90万个单核苷酸多态性进行基因分型，并收集居住在萨摩亚农村地区的2500名成年萨摩亚人的肥胖表型。

DESCRIPTION (provided by applicant): "Genome-wide association studies of adiposity in Samoans" The goal of this new application is to define the genetic variation that underlies obesity and obesity-related phenotypes among adult Samoans. We will use genome-wide association methods to take advantage of newer more powerful techniques to identify single nucleotide polymorphism (SNP) variants that are associated with adiposity phenotypes. We will conduct these studies using a population sample of 2,500 adults from rural areas of Samoa, an independent nation experiencing the nutritional transition. We will replicate the findings from our genome-wide association studies of important SNPs by further analysis in three different study samples: an independent sample of 2,500 Samoans from earlier studies in American Samoa and Samoa, and 1,500 adults from Croatia and 1,000 adults from China. We will then determine the sequences of the important genes and determine the specific genetic variants in the sequenced genes that are associated with obesity in these three samples. Finally, we will explore gene-environment interactions, taking advantage of identified genetic variants in the sequenced regions and the substantial environmental heterogeneity in lifestyle and nutritional behaviors within Samoa. The knowledge gained from this research will aid the development of interventions on obesity amon Samoans including those in the US and may be generalizable to other Pacific Islander groups. PUBLIC HEALTH RELEVANCE: The goal of this application is to define the genetic variation that underlies obesity and obesity-related phenotypes among adult Samoans using genome-wide association methods. Genotyping will be performed using a panel of approximately 900,000 SNPs, and adiposity phenotypes will be collected from 2,500 adult Samoans residing in rural regions of Samoa.