Purchase of two 400 MHz NMR Spectrometers

购买两台 400 MHz 核磁共振波谱仪

• 批准号: 7595662
• 负责人: Mary Fedarko Roberts
• 金额: $ 43.96万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595662
• 关键词: 14 year old; Automation; B-Lymphocytes; Biological Assay; Boston; Cell Nucleus; Cessation of life; Development; Diabetes Mellitus; Enzymes; Faculty; Funding Applicant; Glucose; Glycolysis; Goals; Growth; Mammalian Cell; Methods; Monitor; Natural Product Drug; Nucleic Acids; Pentosephosphate Pathway; Phosphatidylinositols; Phospholipids; Research; Research Personnel; Sampling; Screening procedure; Signal Transduction; System; Time; Tumor Cell Line; United States National Institutes of Health; Update; Variant; catalyst; college; data acquisition; drug development; glucose metabolism; human disease; instrument; instrumentation; member; novel; public health relevance; research study; response; tumor

DESCRIPTION (provided by applicant): Funds are requested to (i) purchase a Varian 400 MHz DirectDrive NMR system with SMS autosampler and (ii) replace a 14-year old Gemini 400 MHz NMR spectrometer with a second Varian 400 MHz DirectDrive console. The existing Oxford magnet on the Gemini 400 is still very serviceable and will be used with the new console. Tied into this upgrade of the NMR facilities is an upgrade of a Varian INOVA 500 to be compatible with the DirectDrive system. These new/upgraded spectrometers will support the analytical efforts of eight NIH-supported faculty members at Boston College. These instruments will have a very profound effect for researchers developing methods for the synthesis of natural products/drugs. Such projects routinely require the acquisition of many NMR spectra, both 1D and simple 2D experiments, to confirm intermediates as well as end products. The updated NMR facility will also allow B.C. chemists to more easily explore nuclei other than 1H and 13C. Of particular note, many more 31P and 19F spectra will be acquired and used in organometallic catalyst characterization, modified nucleic acid synthesis, synthetic phospholipids characterization, and diverse enzyme assays. The requested autosampler will enhance sample throughput since it will maximize the use of long blocks of time that are frequently underutilized on the current outdated spectrometers, and the new automation capabilities will dramatically reduce setup time between data acquisitions and sample changes. Other NIH- supported projects will also benefit from these instruments. 13C-selected 1H-NMR experiments will be used to monitor glucose metabolism in B lymphocytes as a way of exploring how glucose is split between glycolysis and pentose phosphates pathways, and to monitor the response of the tumor cell line U937 to synthetic variants of 3-deoxy-phosphatidylinositol molecules that are thought to target Akt1. PUBLIC HEALTH RELEVANCE: The research that will use this instrumentation has three basic goals: (1) development of novel synthetic methods to produce natural products and drugs applicable to a wide variety of human diseases, (2) screening and further development of drugs to target diabetes and tumors, and (3) exploring how specific mammalian cells respond to growth and death signals.

描述（由申请人提供）：要求资金用于(i)购买带有SMS自动采样器的瓦里安400 MHz DirectDrive核磁共振系统，（ii）用第二台瓦里安400 MHz DirectDrive控制台替换14年的Gemini 400 MHz核磁共振光谱仪。Gemini 400上现有的牛津磁铁仍然非常耐用，将与新的控制台一起使用。与此次核磁共振设施升级相关的是瓦里安INOVA 500的升级，以与DirectDrive系统兼容。这些新的/升级的光谱仪将支持美国国立卫生研究院支持的波士顿学院8名教员的分析工作。这些仪器将对开发天然产物/药物合成方法的研究人员产生非常深远的影响。此类项目通常需要获取许多核磁共振波谱，包括1D和简单的2D实验，以确认中间体和最终产品。更新后的核磁共振设备也将使bc省的化学家更容易探索除1H和13C以外的原子核。特别值得注意的是，将获得更多的31P和19F光谱，并将其用于有机金属催化剂表征、修饰核酸合成、合成磷脂表征和各种酶分析。所要求的自动进样器将提高样品吞吐量，因为它将最大限度地利用当前过时的光谱仪上经常未充分利用的长时间块，并且新的自动化功能将大大减少数据采集和样品更改之间的设置时间。其他NIH支持的项目也将受益于这些仪器。13c选择的1H-NMR实验将用于监测B淋巴细胞中的葡萄糖代谢，作为探索葡萄糖如何在糖酵解和戊糖磷酸途径之间分裂的一种方式，并监测肿瘤细胞系U937对被认为靶向Akt1的3-脱氧磷脂酰肌醇分子合成变体的反应。公共卫生相关性：将使用该仪器的研究有三个基本目标：(1)开发新的合成方法来生产适用于各种人类疾病的天然产物和药物，(2)筛选和进一步开发针对糖尿病和肿瘤的药物，以及(3)探索特定哺乳动物细胞如何对生长和死亡信号作出反应。