LTQ Orbitrap XL Mass Spectrometer

说明（由申请人提供）：申请资金用于购买Thermo Scientific LTQ Orbitrap XL质谱仪。该仪器将成为明尼苏达大学癌症中心分析生物化学核心设施的一部分。它将为该大学提供分析能力，而这种能力在复杂生物基质中的低水平小分子分析中是常规无法获得的。已经确定了9名NIH资助的明尼苏达大学的主要研究人员，他们需要拟议仪器提供的分析能力。他们的主要需求是能够观察和获得准确的质量和结构信息，以识别代谢物，DNA加合物（检测为修饰的DNA碱基），以及复杂生物基质中低水平的其他痕量成分。第二个需求是高分辨率、准确的质量和MSn能力，以观察/定量和确认具有显著化学噪声的色谱痕量中低水平分析物的身份。本申请中概述的大部分研究将作为明尼苏达大学癌症中心致癌和化学预防计划的一部分进行。这项工作的目标是了解烟草产品和人类环境中存在的致癌物质导致人类癌症的机制。这些信息将用于制定预防癌症的实用战略。公共卫生相关性：拟议的仪器将用于研究了解烟草制品和人类环境中存在的致癌物质导致人类癌症的机制。这些信息将用于制定预防癌症的实用策略，并确定特别容易受到致癌物DNA损伤特性影响的个体。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Thermo Scientific LTQ Orbitrap XL mass spectrometer. This instrument would become part of the Analytical Biochemistry Core Facility of the University Of Minnesota Cancer Center. It would provide the university with analytical capabilities that are not routinely available for the analysis of low level small molecule analysis in complex biological matrices. Nine NIH funded principal investigators at the University of Minnesota in need of the analytical capabilities provided by the proposed instrumentation have been identified. Their primary need is the ability to observe and attain accurate mass and structural information to identify metabolites, DNA adducts (detected as modified DNA bases), and other trace constituents at low levels in complex biological matrices. The secondary need is for high resolution accurate mass and MSn capabilities to observe/quantify and confirm the identity of low levels of analyte in chromatographic traces with significant chemical noise. Much of the research outlined in this application will be done as part of the Carcinogenesis and Chemoprevention Program of the University of Minnesota Cancer Center. The goal of this effort is to understand mechanisms by which carcinogens present in tobacco products and the human environment cause cancer in humans. This information will be used to develop practical strategies for cancer prevention. PUBLIC HEALTH RELEVANCE: The proposed instrument will be used for research into understanding mechanisms by which carcinogens present in tobacco products and the human environment cause cancer in humans. This information will be used to develop practical strategies for cancer prevention and identification of individuals particularly susceptible to the DNA damaging properties of carcinogens.