Triple Quadrupole Mass Spectrometer

说明（申请人提供）：申请资金用于购买Thermo Finnigan TSQ Quantum三重四极质谱仪和附带的Thermo Finnigan Surveyor液相色谱系统。该仪器将成为明尼苏达大学癌症中心分析生物化学核心设施的一部分，在该设施中对本提案中概述的致癌物代谢物和DNA加合物进行定量分析。三重四极杆质谱是定量分析的理想方法，因为它具有特异性、灵敏度和定量精度的最佳组合。最近发布的TSQ Quantum仪器在灵敏度和分辨率方面比Thermo Finnigan以前最先进的三重四极杆仪器TSQ 7000有了飞跃。核心设施目前使用TSQ 7000进行设施内完成的所有气相色谱和液相色谱定量分析。对于利血平（一种常用化合物，用于比较大气压电离灵敏度（API）），TSQ Quantum已证明灵敏度比TSQ 7000提高至少15倍。与TSQ 7000的0.7 amu FWHM分辨率相比，它被设计为在0.1 amu分辨率（半峰高全宽，FWHM）下工作。由高分辨率前体选择和/或高分辨率中性损失操作提供的额外选择性将消除与在标称质量分辨率下收集的MS/MS数据中仍然存在的复杂生物基质相关的大部分化学噪声。改进的灵敏度和分辨率将允许在阿托摩尔至飞摩尔水平下测量来自复杂基质（如尿液和血液）的样品中的痕量化合物。明尼苏达大学癌症中心的四名主要研究人员需要TSQ Quantum提供的低检测限和定量精度。这些研究人员研究的一个中心主题是阐明人们因吸烟而患癌症的机制。研究人员从互补的方向攻击这个问题，并为理解常见环境暴露与癌症之间最直接的联系之一构成了一个临界质量。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Thermo Finnigan TSQ Quantum triple quadrupole mass spectrometer and an accompanying Thermo Finnigan Surveyor liquid chromatography system. This instrumentation would become part of the University of Minnesota Cancer Center's Analytical Biochemistry Core facility where the quantitative analyses of carcinogen metabolites and DNA adducts outline in this proposal are performed. Triple quadrupole mass spectrometry is the ideal approach for quantitative analyses, as it presents the best combination of specificity, sensitivity, and quantitative precision. The recently released TSQ Quantum instrument represents a leap forward in sensitivity and resolution over Thermo Finnigan's previous state-of-the-art triple quadrupole instrument, the TSQ 7000. The Core facility currently operates a TSQ 7000 to perform all the gas chromatography and liquid chromatography quantitative analyses done in the facility. The TSQ Quantum has demonstrated a sensitivity improvement of a factor of at least 15 over the TSQ 7000 for reserpine, a commonly used compound for comparisons of atmospheric pressure ionization sensitivity (API). It has been designed to operate at 0.1 amu resolution (full width at half maximum peak height, FWHM) resolution as compared to 0.7 amu FWHM resolutions for the TSQ 7000. The additional selectivity afforded by high resolution precursor selection and/or high resolution neutral loss operation will eliminate much of the chemical noise associated with complex biological matrices still present in MS/MS data collected at nominal mass resolution. The improved sensitivity and resolution will allow for the measurement of trace compounds at attomole to femtomole levels in samples derived from complex matrices such as urine and blood. Four principal investigators at the University of Minnesota Cancer Center are in need of the low limits of detection and quantitative precision the TSQ Quantum provides. A central theme encompassing the research of these investigators is the elucidation of the mechanisms by which people develop cancer from tobacco smoke exposure. The researchers attack the problem from complementary directions and make up a critical mass for understanding what is one of the most direct links between common environmental exposure and cancer.