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Coherent AntiStokes Raman Scattering Microscope

相干反斯托克斯拉曼散射显微镜

基本信息

批准号：
7595285
负责人：
Tim C Lei
金额：
$ 50万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2010-09-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We propose to purchase a Coherent AntiStokes Raman Scattering Microscopy system. This instrument offers novel molecule specific label free imaging of biological samples and tissue. In particular this system will be compatible with intravital microscopy approaches and will be tunable such that it can detect multiple molecules in the same sample. A wide range of investigators involved in NIH clinical and basic research propose to use the system to enhance and develop their interests. These individual projects exploit the full range of benefits that CARS brings to imaging. These include label free non invasive imaging, compatibility with live cell and intravital microscopy. The NIR laser source used in this system is particularly suitable for tissue imaging. Depth resolved imaging is inherent in the CARS technique. This allows mapping of three dimensional tissue or sample volumes with submicron resolution. The CARS microscope has been specified by a diverse range of investigators working on a range of disease related problems. The strength of CARS in imaging lipid deposits and lipid dynamics has been fully exploited by this group. In addition we also propose its use in imaging calcifications such as in the vasculature. The CARS system adds new breadth to research capabilities in the basic sciences and medicine. Its location on a medical research campus ensures its effective and imaginative use. PUBLIC HEALTH RELEVANCE: CARS microscopy is a new method for imaging the three dimensional distribution of target molecules occurring in cells and tissue without the needing to apply an external marker to those molecules. Imaging of live cells and tissues is possible which in turn leads to new understanding of the role of these molecules in dynamic physiological processes and in disease.

描述(申请人提供)：我们建议购买一套相干反斯托克斯拉曼散射显微镜系统。该仪器提供了生物样品和组织的新型分子特定标记的无标记成像。特别是，该系统将与活体显微镜方法兼容，并将是可调的，以便它可以检测同一样本中的多个分子。参与NIH临床和基础研究的广泛研究人员建议使用该系统来增强和发展他们的兴趣。这些单独的项目充分利用了汽车给成像带来的各种好处。这些包括无标记的非侵入性成像、与活细胞和活体显微镜的兼容性。该系统中使用的近红外激光光源特别适合于组织成像。深度分辨成像是CARS技术所固有的。这允许以亚微米分辨率绘制三维组织或样本体积的地图。CARS显微镜是由致力于一系列疾病相关问题的各种研究人员指定的。这组人充分利用了CARS在成像脂质沉积和脂质动力学方面的优势。此外，我们还建议将其用于钙化成像，例如在血管系统中。CARS系统为基础科学和医学的研究能力增加了新的广度。它位于医学研究园区，确保了它的有效和富有想象力的使用。公共卫生相关性：CARS显微镜是一种新的方法，可以成像出现在细胞和组织中的目标分子的三维分布，而不需要对这些分子应用外部标记。对活细胞和组织进行成像是可能的，这反过来又导致对这些分子在动态生理过程和疾病中的作用的新的理解。